Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composite Tbeta4 collagen-chitosan tissue engineering scaffold as well as preparation method and application thereof

A technology of tissue engineering scaffold and chitosan, which is applied in medical science, prosthesis, etc., can solve the problems of low efficiency, unfavorable skin hard-healing wound repair process, skin appendage regeneration, etc., so as to improve transplantation efficiency, promote rapid angiogenesis and Excellent effect on re-epithelialization, porosity and three-dimensional porous structure

Inactive Publication Date: 2015-09-02
GENERAL HOSPITAL OF PLA
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its efficiency in treating diabetic skin refractory wounds is low, which is not conducive to promoting the repair process of refractory skin wounds and the regeneration of skin appendages

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite Tbeta4 collagen-chitosan tissue engineering scaffold as well as preparation method and application thereof
  • Composite Tbeta4 collagen-chitosan tissue engineering scaffold as well as preparation method and application thereof
  • Composite Tbeta4 collagen-chitosan tissue engineering scaffold as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] In the first aspect, the present invention provides a preparation method of a collagen-chitosan tissue engineering scaffold compounded with Tβ4, the method comprising: preparing a collagen-chitosan porous scaffold, and soaking the collagen-chitosan porous scaffold in Tβ4 In the solution, a collagen-chitosan porous scaffold complexed with Tβ4 is obtained; then the collagen-chitosan porous scaffold complexed with Tβ4 is sequentially subjected to chemical cross-linking treatment and freeze-drying treatment.

[0021] In the preparation method of the present invention, the method for preparing the collagen-chitosan porous scaffold is not particularly limited, and may be various preparation methods commonly used in the art. But, the inventor of the present invention finds in research, when preparing collagen-chitosan porous support, control the specific concentration of collagen and chitosan and the pH value of collagen-chitosan solution in collagen-chitosan solution The cumu...

Embodiment

[0038] The following examples will further illustrate the present invention, but do not limit the present invention thereby.

[0039] The experimental methods in the following examples and comparative examples, unless otherwise specified, are conventional methods in the art. The experimental materials used in the following examples and comparative examples are commercially available unless otherwise specified.

[0040] Chitosan powder was purchased from Sigma Company.

[0041] Tβ4 was purchased from ProSpec Corporation.

[0042] The scanning electron microscope was purchased from Hitachi, Japan, model TM100.

[0043] The preparation method of EDC / NHS cross-linking agent is: dissolve EDC, NHS respectively in 95% ethanol solution to concentration to be 33nmol / L and 8nmol / L, obtain EDC solution and NHS solution, then equal volume of EDC solution and NHS solution mix.

preparation example

[0045] This preparation example is used to illustrate the extraction method of collagen powder.

[0046] (1) Take 10 g of fresh bovine tendon, remove the surrounding connective tissue, and cut the bovine tendon into 1-2mm sized tissue pieces.

[0047] (2) Put the tissue block into 0.1% Na 2 CO 3 Soak in the solution for 2h, rinse with distilled water 3 times, 10min each time.

[0048] (3) Soak the tissue block in 0.5% Tris-HCl (pH=7.5) solution containing 1 mol / L NaCl for 12 hours, rinse with distilled water for 3 times, 10 minutes each time.

[0049] (4) Put the tissue pieces into 250 mL of 0.05 mol / L acetic acid solution containing 500 mg of pepsin, soak at 4°C for 72 hours, and stir repeatedly until fully digested into a transparent paste.

[0050] (5) The mixture was centrifuged at 5000 g for 20 min at 4° C., and the supernatant was taken.

[0051] (6) Perform the operations of steps (4) and (5) on the centrifuged sediment in step (5), and repeat the operation 2 times....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Concentrationaaaaaaaaaa
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of biological products, and discloses a composite Tbeta4 collagen-chitosan tissue engineering scaffold as well as a preparation method and application thereof in preparation of skin substitutes. The preparation method disclosed by the invention comprises the following steps: preparing a collagen-chitosan porous scaffold, soaking the collagen-chitosan porous scaffold into a Tbeta4 solution, so as to obtain the composite Tbeta4 collagen-chitosan porous scaffold; and sequentially carrying out chemical crosslinking treatment and freeze drying treatment on the composite Tbeta4 collagen-chitosan tissue engineering scaffold. According to the composite Tbeta4 collagen-chitosan tissue engineering scaffold disclosed by the invention, regeneration, reconstruction, repairing and healing of hard-to-heal wounds of skin caused by diabetes mellitus and the like can be obviously and effectively promoted.

Description

technical field [0001] The invention relates to the field of biological products, in particular to a Tβ4-composite collagen-chitosan tissue engineering scaffold and its preparation method and application. Background technique [0002] Because the tissue cells of diabetic patients are in a high-glucose environment for a long time, the local skin tissue cells and proteins are glycosylated, causing abnormal vascular endothelial function and impaired response; the function of macrophages is weakened, and the inflammatory response is prolonged and unbalanced; growth The lack or damage of factors, the reduction of collagen tissue, the disorder of epidermal barrier function, and the reduction of granulation tissue make the ulcer difficult to repair. And the research on the treatment of refractory skin wounds caused by diabetes has not yet achieved a major breakthrough. [0003] The collagen-chitosan tissue engineering scaffold based on collagen and chitosan is a relatively complet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/24A61L27/20A61L27/54A61L27/60
Inventor 韩为东付小兵郝好杰遆冬冬佟川郑敬曦
Owner GENERAL HOSPITAL OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com