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Composition for skin regeneration, containing a secretion in the culture of an embryonic stem cell-derived endothelial progenitor cell or fractions thereof, and use thereof

A technology of embryonic stem cells and endothelial progenitor cells, which is applied to the skin regeneration components and the fields of use thereof comprising the cultured secretions of embryonic stem cell-derived endothelial progenitor cells or their isolates, so as to achieve effective cure, reduce limitations, and promote re-epithelialization Effect

Inactive Publication Date: 2012-01-25
车比奥及戴奥斯泰有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In addition, the combination of the cultured secretion or its constituent factors having such a skin regeneration function makes it possible to use new cosmetics for wrinkle improvement or skin aging prevention.

Method used

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  • Composition for skin regeneration, containing a secretion in the culture of an embryonic stem cell-derived endothelial progenitor cell or fractions thereof, and use thereof
  • Composition for skin regeneration, containing a secretion in the culture of an embryonic stem cell-derived endothelial progenitor cell or fractions thereof, and use thereof
  • Composition for skin regeneration, containing a secretion in the culture of an embryonic stem cell-derived endothelial progenitor cell or fractions thereof, and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Acquisition and concentration of human embryonic stem cell-derived endothelial progenitor cell culture secretion

[0049] On a culture dish coated with collagen, cultivate the above-mentioned human embryonic stem cell-derived endothelial progenitor cells in EGM-2 / MV (Cambrex) medium, and pass through 48 cells when the cell concentration distribution on the culture dish is about 70-80%. Hour culture to obtain human embryonic stem cell-derived endothelial progenitor cell culture secretion concentrate. The obtained secretions were concentrated 50-fold using the TFT membrane concentration system to obtain high-concentration cultured secretion concentrates.

[0050] (2) Production of trauma and burn animal models

[0051] figure 2 It is a photo and a simulation diagram showing the process of making and applying a trauma animal model for the verification of the effect of trauma treatment.

[0052] The aforementioned wound animal model used to verify the effect of ...

experiment example 1

[0061] The active ingredients of the human embryonic stem cell-derived endothelial progenitor cell culture secretion concentrate (50-fold concentrated high-concentration concentrate) cultured under the conditions specified in (1) of Example 1 were determined using a multiplex cytokine array. analysis.

[0062] figure 1 It is an analysis table showing the composition and effective substance content of the human embryonic stem cell-derived endothelial progenitor cell culture secretion concentrate according to the present invention.

[0063] Such asfigure 1 As shown, it can be seen that the 50-fold concentrated human embryonic stem cell-derived endothelial progenitor cell culture secretion concentrate contains a large amount of EGF, FGF-2, Fractalkine, GM-CSF, IL-6, IL-8, IL-9, IP-10 , MCP-1, PDGF-AA, PDGF-BB, VEGF and other active ingredients for promoting angiogenesis and wound healing.

[0064] From the above results, it can be seen that the human embryonic stem cell-derived...

experiment example 2

[0066] After the trauma animal model was made by the method shown in (2) of Example 1, the human embryonic stem cell-derived endothelial progenitor cell culture secretion concentrate and other comparison groups were applied to the inside of the silicon ring on the back of the trauma animal model (refer to figure 2 ).

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Abstract

The present invention relates to a composition for skin regeneration, using a culture medium or a secretion in the culture of an embryonic stem cell-derived endothelial progenitor cell, and to the use thereof. As the present invention uses the secretion in the culture as a medicine and not the embryonic stem cell-derived endothelial progenitor cell itself, the risk of teratoma formation is prevented, and angiogenic activity is promoted to achieve improved effectiveness in healing wounds and burn wounds. The composition of the present invention, when used as a material in cosmetics, increases collagen synthesis and thus prevents skin aging. Particularly, the composition of the present invention in which the secretion in the culture is concentrated into a high concentration provides superior wound-healing effects as compared to a conventional human growth hormone (hGH).

Description

technical field [0001] The present invention relates to a composition for skin regeneration comprising embryonic stem cell derived endothelial progenitor cells cultured secretions or fractions thereof and its use. The bioprotein isolated from the culture medium of endothelial progenitor cells is suitable for wounds, burns, or skin shaping, and it is a technology for functional cosmetics for wrinkle improvement or skin aging prevention while performing treatment through skin regeneration. Background technique [0002] Most of the current wound healing and burn wound healing use therapeutic agents, dressing gauze or artificial skin grafting. In the case of gauze used for therapeutic agents, although there is an advantage in the transmission of effective factors and reduction of the risk of infection in the gauze composed of synthetic and natural polymers to absorb effective factors for wound healing, continuous treatment has not been carried out at the same time. In the case ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61Q19/08A61K8/98A61K9/20A61P17/02
CPCA61Q19/08A61K8/982A61P17/00A61P17/02
Inventor 郑炯敏金珠美李旻智洪起成成钟赫
Owner 车比奥及戴奥斯泰有限公司
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