The invention discloses a method for making a bamboo fiber no-ironing shirt. The method comprises the steps that first bamboo fiber fabric is placed into souring and bleaching liquid and is soured and bleached for 30-60min, wherein souring and bleaching temperature is 90-100 DEG C, a liquor ratio is 1: 30-35, the souring and bleaching liquid contains 30% of hydrogen peroxide with concentration being 6-10g / L, sodium silicate with the concentration being 5-10g / L, penetrating agents with the concentration being 5-8g / L, scouring agents with the concentration being 3-5g / L and oxygen bleaching stabilizer with the concentration being 5-8g / L; the bamboo fiber fabric is cleaned and dried after the scouring and bleaching, then the processed bamboo fiber fabric is tailored and sewed and a ready-made garment is obtained; then a no-ironing treatment is carried out on the manufactured garment, wherein according to the no-ironing treatment, bidirectional stereoscopic type atomization resin absorption, pre-drying, medium or high temperature micro-pressure stereoscopic shaping and finishing, washing, drying, cooling and re-shaping are conducted on the garment. The method for making the bamboo fiber no-ironing shirt can effectively improve the crease-resistant property of bamboo fiber and have a better shrink-proof and no-ironing effect, a manufactured shirt has the advantages of being not deformed easily, and not easy to shrink and the shirt is not easily deformed, not easy to shrink and yellowing, stable in size, soft in handle, uniform in color and lustre, and cool and comfortable to wear.