The invention relates to an isolation fence and a mounting method thereof, and belongs to the technical field of road facilities. The isolation fence comprises a plurality of vertical columns arrangedmutually at intervals, a frame body is arranged between every two adjacent vertical columns, isolation nets are arranged in the frame bodies in a sealing mode, fixing plates are arranged on the positions, on the upper parts and the lower parts of the vertical columns, of the two sides of each vertical column correspondingly, first springs are arranged on the positions, on the upper parts and thelower parts of the frame bodies, of the two sides of the frame bodies correspondingly, the ends, away from the frame bodies, of the first springs are provided with abutting plates in a mode of being perpendicular to the first springs, through holes used for allowing the first springs to penetrate are formed in the fixing plates, and the edges, away from the vertical columns, of the fixing plates are provided with channels in a mode of facing the fixing plates, wherein the channels are used for allowing the first springs to move into the through holes. The first springs move into the through holes, thus the isolation nets driven by the frame bodies can be fixed, the fixing mode is convenient and simple, and the mounting efficiency of mounting personnel is improved.