The invention discloses a culture medium used for improving flavonoid compounds and polysaccharides. The culture medium comprises raw materials including cantaloupe juice, muskmelon juice, prune juice, grape juice, tea polyphenol, potassium nitrate, ferric phosphate, ammonium nitrate, calcium nitrate, magnesium nitrate, potassium iodate, sodium borate, potassium molybdate, zinc chloride, manganous sulfate monohydrate, copper chloride, ferric nitrate, vitamins, amino acid, indolebutyric acid, gibberellin, 6-benzyl gland fat poison, isoamyl amino purine, naphthylacetic acid, agarophyte, kinetin, cosolvent, a dispenser, compatilizer, stabilizer, antiseptics and water. The culture medium is reasonable in matching, complete in nutrition and capable of rapidly promoting roots to grow stems, transplanting survival rate is increased, accumulation of the flavonoid compounds can be promoted, and economic benefit is improved. The preparation method of the culture medium is simple in operation, and industrialization, standardization and standardization production is easy to realize.