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Anoectochilus roxburghii tissue culture seedling culture medium

A technology of culture medium and tissue culture seedlings, which is applied in the field of culture medium of clematis tissue culture seedlings, can solve the problems of reducing the survival rate of transplanting, root damage, and increasing production costs, so as to improve the survival rate of transplanting and quickly promote roots Stems, with reasonable effect

Inactive Publication Date: 2017-01-11
唐春艳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a culture medium for tissue cultured seedlings of A. aurantiaceae to solve the problem that most of the existing tissue culture mediums use solid medium, which not only increases the production cost, but also easily causes root damage when taking seedlings, which reduces the survival of transplanting. rate problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A culture medium for tissue culture seedlings of Clematis clematis, comprising the following raw materials in parts by weight: 250 parts of cantaloupe juice, 3 parts of citric acid, 0.1 part of o-benzoylsulfonimide, 2.5 parts of apple polyphenols, potassium salt 3.5 parts, 2.6 parts phosphorus salt, 2.2 parts nitrogen salt, 1 part calcium salt, 1 part magnesium salt, 0.5 part iodine salt, 0.3 part boron salt, 0.25 part molybdenum salt, 0.35 part zinc salt, 0.2 part manganese salt, copper salt 0.15 parts, 0.16 parts of iron salts, 0.2 parts of vitamins, 0.16 parts of amino acids, 0.1 parts of indole butyric acid, 0.1 parts of gibberellin, 0.08 parts of 6-benzyl adenine, 0.12 parts of naphthaleneacetic acid, 0.08 parts of kinetin, 02 parts of asparagine, 3000 parts of water.

[0025] The potassium salt is potassium chloride; the phosphorus salt is calcium phosphate; the nitrogen salt is ammonium bicarbonate; the calcium salt is calcium glycinate; the magnesium salt is mag...

Embodiment 2

[0028] A culture medium for tissue culture seedlings of Clematis clematis, comprising the following raw materials in parts by weight: 130 parts of cantaloupe juice, 2.3 parts of citric acid, 0.08 parts of o-benzoylsulfonimide, 1.6 parts of apple polyphenols, potassium salt 2.6 parts, 1.5 parts of phosphorus salt, 1.2 parts of nitrogen salt, 0.8 part of calcium salt, 0.7 part of magnesium salt, 0.4 part of iodine salt, 0.2 part of boron salt, 0.2 part of molybdenum salt, 0.3 part of zinc salt, 0.1 part of manganese salt, copper salt 0.1 part, 0.1 part of iron salt, 0.12 part of vitamin, 0.1 part of amino acid, 0.08 part of indole butyric acid, 0.08 part of gibberellin, 0.07 part of 6-benzyl adenine, 0.08 part of naphthaleneacetic acid, 0.05 part of kinetin, 0.14 parts of asparagine, 2500 parts of water.

[0029] The potassium salt is potassium chloride; the phosphorus salt is calcium phosphate; the nitrogen salt is ammonium bicarbonate; the calcium salt is calcium glycinate; th...

Embodiment 3

[0032] A culture medium for tissue culture seedlings of Clematis clematis, comprising the following raw materials in parts by weight: 350 parts of cantaloupe juice, 3.6 parts of citric acid, 0.12 parts of o-benzoylsulfonimide, 3 parts of apple polyphenols, potassium salt 4.2 parts, 3.8 parts of phosphorus salt, 2.6 parts of nitrogen salt, 1.3 parts of calcium salt, 1.2 parts of magnesium salt, 0.6 part of iodine salt, 0.4 part of boron salt, 0.3 part of molybdenum salt, 0.4 part of zinc salt, 0.3 part of manganese salt, copper salt 0.2 parts, 0.2 parts of iron salts, 0.28 parts of vitamins, 0.22 parts of amino acids, 0.14 parts of indole butyric acid, 0.12 parts of gibberellin, 0.09 parts of 6-benzyladenophyllin, 0.14 parts of naphthaleneacetic acid, 0.09 parts of kinetin, 0.26 parts of asparagine, 364000 parts of water.

[0033] The potassium salt is potassium chloride; the phosphorus salt is calcium phosphate; the nitrogen salt is ammonium bicarbonate; the calcium salt is ca...

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Abstract

The invention discloses an anoectochilus roxburghii tissue culture seedling culture medium which is prepared from the following raw materials: cantaloupe juice, citric acid, O-benzoylsulfonyl imide, apple polyphenol, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodate, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, ferric salt, vitamin, amino acid, indolebutyric acid, gibberellin, 6 - benzyl gland fat poison, naphthylacetic acid, kinetin, asparagine and water. The anoectochilus roxburghii tissue culture seedling culture medium is reasonable in matching and complete in nutrients, can rapidly promote roots and stems, improve the content of anoectochilus roxburghii polysaccharide, flavonoid compound and alkaloid, shorten the culture period of anoectochilus roxburghii, improve the survival rate of transplanted anoectochilus roxburghii, and lower the production cost.

Description

technical field [0001] The invention belongs to the technical field of clematis planting, and in particular relates to a culture medium for tissue culture seedlings of clematis. Background technique [0002] Clematis clematis, also known as golden clematis, jinbuhuan, bird ginseng, etc., is a rare and rare Chinese herbal medicine. It is a perennial herb of the family Orchidaceae. It is mainly distributed in Fujian, Taiwan, Zhejiang, Jiangxi, Guangdong and Guangxi in China. The whole herb is used as medicine, which has the functions of cooling and annealing, cooling the blood and strengthening the lungs, detoxifying and detoxifying, and nourishing and strengthening. It has extensive medicinal value among the people. ", Taiwan is called "the king of Chinese medicine"; It has broad prospects for development and utilization. [0003] Clematis is a shade plant, and its light saturation point is low in the natural state. Wild clematis is distributed between 300-1200m above sea l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 唐春艳
Owner 唐春艳
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