Fermenting culture process of produicng Cordyceps extract
A technology of fermentation culture and cordycepin, applied in the direction of plant cells, fungi, etc., to achieve the effect of increasing yield, shortening culture period and reducing cost
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[0029] A 2L airlift cell reactor (purchased from the Biochemical Center of the Chinese Academy of Sciences) was used as a seed tank, and the liquid seeds were cultured at 26°C±1 for 96 hours, at a temperature of 24°C, and scattered light. The liquid seed medium consists of: 200 grams of potatoes, 10 grams of glucose, KH 2 PO 4 2 g, MgSO 4 1 gram, 5 grams of peptone, 2 grams of yeast extract.
[0030] After 5 days of liquid seed cultivation, select seed liquid with clear culture color, uniform bacterial balls, and a diameter of about 3mm, and inoculate it into a 20L air-lift fermenter at a 10% inoculum size, and cultivate it under sterile conditions. The cultivation of fermentation culture The base composition is the same as that of the above-mentioned seed liquid culture medium, the culture temperature is 24° C., and the culture time is 5 days, and the ventilation rate is 200 ml / min.
[0031] After the above-mentioned fermentation culture, the culture product was dried belo...
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