Preparation method of special tissue culture solution for dendrobium candidum

Abstract

The invention discloses a preparation method of a special tissue culture solution for dendrobium candidum. The preparation method comprises the following steps: dissolving potassium iodide, manganese sulfate, zinc sulfate, boric acid, sodium molybdate, copper sulfate and cobalt chloride to obtain a first component; dissolving ferrous sulfate and disodium edetate dehydrate to obtain a second component; dissolving inositol, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine to obtain a third component; weighing peeled potatoes, adding the potatoes in a food processer, stirring to obtain mashed potato, adding coconut milk in the mashed potato, and uniformly mixing to obtain a material A; adding ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate and monopotassium phosphate in water to dissolve to obtain a material B; heating the material A, and adding the material B when the material A is heated, uniformly stirring, sequentially adding the first component, the second component and the third component, continuously adding brown sugar and agar, uniformly stirring, and regulating a pH value to obtain a mixed material; packaging the mixed material to sterilize at a high temperature and under high pressure, cooling the sterilized material to a room temperature to obtain the special tissue culture solution for the dendrobium candidum.

Description

Technical field [0001] The invention relates to the technical field of plant culture, in particular to a method for preparing a special tissue culture solution for Dendrobium candidum Background technique [0002] Dendrobium is a perennial epiphytic herb of the genus Dendrobium of Orchidaceae, and it is a precious traditional Chinese medicinal material commonly used in my country. As early as the "Shen Nong's Materia Medica" and "Compendium of Materia Medica", it was listed as top-grade medicinal materials. Dendrobium is used as medicine with stems and contains many alkaloids such as dendrobine, dendrobium, dendrobine, dendrobium, etc; Cardiovascular and ophthalmological diseases have obvious therapeutic effects. Modern medicine has proved that Dendrobium can promote blood circulation and enhance heart function; promote gastrointestinal motility and enhance digestion; it can significantly increase the level of SOP (superoxide dismutase) in the human body and reduce LPO (lipid p...

AI Technical Summary

Problems solved by technology

The tender stems of a few varieties such as Dendrobium candidum are twisted into spiral or spring-shaped and dried in the sun. The commercial product is called Dendrobium earrings. However, due to the slow growth cycle, it is extremely difficult to survive. The wild Dendrobium candidum is on the verge of extinction, and the survival rate of artificial seedlings is low.