47results about How to "Ensure healthy and sustainable development" patented technology

The invention relates to a method for artificially inducing resistance to jujube witches broom, and the method comprises the following steps: longitudinally cutting a cut at the lower end of the stem section of a cultured plantlet in a healthy group of a susceptible variety, wherein the stem section is utilized as a scion; removing leaves and axillary bus from the stem section of the cultured plantlet in a group infected with the jujube witches broom, and cutting one surface of the upper end of the stem section into a diagonal plane, wherein the stem section is utilized as a rootstock; then inserting the scion, aligning the rootstock with a scion formation layer, and using an aluminum foil to bind a grafting port, thus acquiring a micro-grafted plantlet; inserting into an MS (Murashige and Skoog) culture medium without any plant growth regulator for culture; adding 1mg / ml of tetracycline to kill pathogens in the micro-grafted plantlet after graft survival and afference of the pathogens into the scion, cutting the scion, transferring into the MS culture medium which is additionally added with 1.0mg / mL of BA (6-benzyl adenine) and 0.5mg / mL of IBA (indole-3-butyric acid) for rejuvenation, and further grafting the scion after the rejuvenation onto the rootstock of the cultured plantlet in the group with the jujube witches broom; and continuously performing the induction containingmicro-grafting inoculation, processing with the tetracycline, rejuvenation and micro-grafting inoculation above three times, thus obtaining a plant with resistance to the jujube witches broom. By utilizing the method, the plant with the resistance to the jujube witches broom can be rapidly induced and obtained. By adopting the method, the resistance to the jujube witches broom of jujube witches broom-sensitive good jujube tree varieties can be rapidly improved in an orientation way, thus the method provided by the invention has very broad application prospects.

The invention belongs to the technical field of avian virus detection, and discloses a GeXP primer group and a kit capable of identifying an H5 subtype avian influenza virus and four different NA subtypes thereof at the same time. The inventor researches and designs six pairs of specific primers and one pair of universal primers based on a GeXP system, and builds the GeXP kit capable of identifying the H5 subtype avian influenza virus and four different NA subtypes thereof at the same time accordingly. Through adoption of the GeXP rapid detection kit, the H5 subtype avian influenza virus and the four different NA subtypes thereof can be identified at the same time, and the sensitivity is 102 copies per microliter. The GeXP rapid detection kit has the advantages of high flux, high specificity, high sensitivity, rapidness and the like, and has important significance to epidemiological investigation and differential diagnosis of the H5 subtype avian influenza virus.

The invention discloses a strain of Ralstonia peterii EZ‑50, its metabolites and applications thereof, aiming to solve the technical problem of poor rice sheath blight control effect in the prior art. Ralstonia pickettii (Ralstonia pickettii) CGMCC NO.20622 of the present invention can strongly inhibit Rhizoctonia solani, and can be used to prevent and treat plant diseases caused by Rhizoctonia solani, thereby achieving the goal of reducing pesticide application Purpose, to ensure the sustainable development of agriculture, has broad application prospects; the present invention can metabolize and secrete multiple antibacterial active substances ethyl benzoate, 6-methyl-5-heptene-2 -ketone, 2-ethyl-1-hexanol, dimethyl disulfide, and these volatile substances have a good inhibitory effect on Rhizoctonia solani.

The invention discloses a cluster software system and method based on a data bus, including: a server layer, through a bus mechanism, using a virtualization technology for a hardware server cluster, allocating set resources according to the requirements of distributed components, and establishing its corresponding container, through The container realizes the isolation and decoupling of each component; the hardware server cluster includes: a cluster composed of hardware servers composed of web servers, data servers, and communication servers; each hardware server is used as a single node, and the nodes in the cluster share communication and memory Space and data processing functions; a single node failure will not affect the normal operation of the entire system. The present invention realizes the isolation and decoupling of each component through the container, and each performs its own duties to jointly form a distributed functional system of the system. Each server is used as a single node of the redundant backup cluster. When a machine fails, it is only equivalent to the loss of a part of the hardware capabilities of the platform, and will not affect the normal operation of the entire system.

The invention belongs to the technical field of poultry virus detection and discloses a GeXP rapid detection primer group and kit for identifying combination of three types of infectious H5 subtype avian influenza viruses synchronously and application of the primer group and kit. According to the GeXP rapid detection primer group and kit and application of the primer group, five pairs of specific primers and one pair of universal primer are researched and designed based on a GeXP system, and accordingly the GeXP rapid detection kit for identifying combination of three types of infectious H5 subtype avian influenza viruses synchronously is established. By means of the GeXP rapid detection primer group and kit and application of the primer group and kit, three types of infectious H5 subtype H5N1, H5N2 and H5N6 avian influenza viruses can be identified synchronously, and the sensitivity is 102 copy / micron L. The GeXP rapid detection primer group and kit and application of the primer group have the advantages of being high in flux, high in specificity, high in sensitivity, high in speed and the like and have significance to epidemiological investigation and differential diagnosis of three types of H5 subtype avian influenza viruses.

The invention belongs to the technical field of detection of avian influenza viruses and discloses a GeXP quick detection primer set and kit for identifying simultaneously major epidemic subtypes of avian influenza and their application. Seven pairs of specific primers and one pair of universal primers are designed based on the study on a GeXP system, and the GeXP kit for identifying simultaneously the major subtypes of avian influenza is established accordingly. The primer set and kit can be used to identify the subtypes H5, H7 and H9 and different subtypes of combined avian influenza, with a sensitivity of 102 copies per MuL. The primer set and kit have the advantages of high throughput, high specificity, high sensitivity, high speed and the like and are significant to the epidemiological investigation of major epidemic subtypes of avian influenza and their prevention and control, as well as the guarantee on the healthy substantial development of aviculture.

The invention belongs to the technical field of agriculture, and particularly relates to a polypeptide multi-element organic fertilizer for raising chickens. The polypeptide multi-element organic fertilizer is prepared from polypeptide, ferrous sulfate, zinc sulfate, ammonium molybdate, calcium nitrate, boric acid and water. The polypeptide multi-element organic fertilizer uses a chemical bondingfunction of the polypeptide to realize the chelation of all elements, and promotes the absorption of mineral nutrition for the chickens and improves the chicken immunity and the quality of chicken meat and eggs while reducing the used amount of antibiotics and hormones; besides, the polypeptide multi-element organic fertilizer is favorable for the non-pollution production of the chicken meat and the eggs, has the characteristics of low price, convenient use, obvious effect and the like, and provides a guarantee for the healthy and sustainable development of the poultry industry.