50results about How to "Effective research" patented technology

Preferred systems for assisting clearance of an acutely thrombosed artery substantially surrounded by boney external body surfaces which are resistant to deformative displacement relative to the thrombosed artery by the application of external percussive force are described. The method consists of applying targeted, localized, non-invasive, high infrasonic to low sonic frequency vibratory percussion with a serial impact frequency much greater than the pulse rate of a patient being treated, the percussion directed towards a remote, preferably superficial “target vessel” residing palpably close to the skin surface. Marked vessel deformations with resultant blood pressure and flow fluctuations are thereby induced by the percussion within the target vessel which propagate to the acutely thrombosed artery to provide localized agitation and turbulence to assist thrombolytic and / or IV microbubble delivery and effectiveness in facilitating reperfusion. Preferred apparatus for treatment of ST elevation myocardial infarction, acute ischemic stroke and acute pulmonary embolus are presented.

The invention discloses an experimental apparatus for a non-constant debris flow. The experimental apparatus comprises a water storage tank, a hopper placed with debris, a frame for installing the hopper, a tank which is obliquely arranged and has an adjustable oblique angle, and an aggregate pool for accommodating the materials from the tank, wherein the inlet of the tank is higher than the outlet of the tank, the water outlet of the water storage tank is connected with the inlet of the tank, the water in the water storage tank and the debris in the hopper converge at the inlet of the tank to form the experimental debris flow fluid; then the debris flow fluid flows into the aggregate pool along the tank; and the bottom plate of the tank is provided with a pressure sensor, and the side plates of the tank are transparent. The invention has the advantages that: the experimental apparatus is simple in operation and can be used for effectively studying the movement characteristics of the non-constant debris flow.

A tracing method for the intermedullary filling stem cells includes such steps as using transfectant to modify superparamagnetic iron oxide to make it carry positive charges, using it to mark the cultured intermedullary filling stem cells, inoculating said cells onto scaffold, transplanting it in the body of animal, and using NMR imaging instrument for tracing the cells in animal body.

The invention relates to a fluorescent probe for ratio detection of hypochlorous acid and application thereof in biological systems. Specifically, in the presence of hypochlorous acid, fluorescence wavelength of the probe may change. The invention provides the fluorescent probe for selective ratio detection of the hypochlorous acid in solution systems, cells, animal tissues or individual animals. The fluorescent probe adopts a cyanine dyes as a fluorescent matrix, a secondary amine is introduced onto the middle position of the cyanine matrix, selective detection of the hypochlorous acid can be realized by using the properties that the cyanine matrix is high in electron cloud density and easy to be oxidized by the hypochlorous acid; after the cyanine matrix is oxidized by the hypochlorous acid, blue shift of the fluorescence wavelength of the probe is occurred, the intensity of long wavelength fluorescent light is decreased gradually along with the increase of the hypochlorous acid content, the intensity of newly-occurred short wavelength fluorescent light is gradually increased, and the fluorescent probe is used for ratio detection of the hypochlorous acid.

The invention discloses an SON (Silicon on Nothing) deformation mechanism study method based on a phase field model at a high temperature. The method comprises the following steps of 1, putting a sample, i.e., an SON material at a certain temperature so that the sample becomes soft, and then making the sample into a U-shaped cylindrical hole shape; 2, putting the SON material into a high-temperature environment to be processed for several minutes for obtaining the SON form change state in different time periods; and 3, building a system model of the SON at the high temperature. According to the method, the SON deformation mechanism at the high temperature is studied by the method of integrating the experiment study and the simulation model for the first time. The SON forming process is controlled by using the high-temperature atomic diffusion motion; through the simulation model, the SON forming change can be more directly observed; and thus, a novel idea for processing the SON is provided.

The invention discloses a phase-field-model-based study method of using laser to control silicon substrate surface morphology. The method comprises the first step of adopting a silicon clamp to clamp a silicon substrate with a micro-nano structure under an atomic force microscope; the second step of using the laser to irradiate a suspending end of the silicon substrate for seconds, and observing the silicon substrate surface morphology of the silicon substrate and measuring the corresponding dimension; the third step of simulating a silicon atom diffusion behavior based on irradiation of the laser and the evolution process of the silicon substrate surface morphology of a nano structure under a phase-field model.

The invention relates to the modeling technology, and aims to provide a modeling method of a virtual synchronous generator taking an excitation circuit into consideration. Based on a three-phase bridge inverter topological structure, a power loop of the virtual synchronous generator is analyzed by neglecting an inner ring voltage and a current loop in a control system; by referring to a flux linkage equation, a voltage equation, a torque equation, a power equation and a motion equation of a traditional synchronous machine, a simplified motor model is proposed by further neglecting a derivativeterm and a zero sequence term in the model; in the model, the excitation current is calculated in a virtual mode by utilizing a reactive power deviation value and an excitation coefficient, and an excitation circuit is constructed; and the obtained motor model is expressed in a small signal disturbance mode, so that a low-order small signal model of the virtual synchronous generator taking the excitation circuit into consideration is obtained. According to the method, the grid-connected topological structure of the common inverter does not need to be changed, and a common grid-connected inverter which is mature and wide in application can be used for carrying out model research, so that the model test is completed under the condition that additional hardware cost is not increased.

The present disclosure provides methods for genetically modifying lymphocytes and methods for performing adoptive cellular therapy that include transducing T cells and / or NK cells. The methods can include inhibitory RNA molecule(s) and / or engineered signaling polypeptides that can include a lymphoproliferative element, and / or a chimeric antigen receptor (CAR), for example a microenvironment restricted biologic CAR (MRB-CAR). Additional elements of such engineered signaling polypeptides are provided herein, such as those that drive proliferation and regulatory elements therefor, as well as replication incompetent recombinant retroviral particles and packaging cell lines and methods of making the same. Numerous elements and methods for regulating transduced and / or genetically modified T cells and / or NK cells are provided, such as, for example, those including riboswitches, MRB-CARs, recognition domains, and / or pH-modulating agents.

The invention discloses a multi-parking-lot multi-vehicle-model vehicle path planning method. The method comprises the following steps: 1) establishing an objective function by taking the lowest totalcost of vehicle distribution as an objective; 2) encoding and redefining an operation mode; 3) initializing a population; 4) calculating the fitness of the artificial fish, and recording the optimalindividual; 5) evaluating each fish, selecting the behavior of each fish at the same time, and selecting the behavior with the highest fitness improvement to execute; 6) calculating the fitness of theobtained new population, and updating the optimal solution in the population; 7) performing annealing search on the optimal solution of the population; 8) ending the primary iteration; 9) judging whether a termination condition is met or not, and if so, executing the step 10; otherwise, returning to execute the step 5, and carrying out a new round of iteration; and 10) decoding the finally recorded optimal individual, calculating the reciprocal of the fitness of the optimal individual, i.e., the value of the target function, and after decoding, taking the client between two same vehicle numbers in the obtained decoding sequence as the distribution route of the vehicle, and outputting an optimal scheme when the unused vehicle does not appear in the decoding sequence.

The invention discloses a haze particle formation simulator which comprises a combustion furnace, a control console, a growth simulation chamber, a light shield, a sucking pump, a flow meter, a three-way valve with a stop valve, a haze particle collector and an air purifier, wherein the growth simulation chamber is fixed on the control console; the combustion furnace, the growth simulation chamberand the air purifier are connected into a system by the sucking pump; the three-way valve with the stop valve realizes independent operation of a smoke dust source supply channel and a haze particlecollection channel; multiple feeding holes and an exhaust valve are formed in the growth simulation chamber, and the growth simulation chamber is equipped with temperature, humidity, airspeed and light intensity detection sensors as well as a light source, a fan and a supercharging balloon; and a temperature control device, a humidifier and a pressure gage are arranged in the control console. By detecting and regulating the temperature, pressure, humidity, airspeed and light intensity in the growth simulation chamber, the outdoor environment formed by haze is simulated, a research is conductedon formation of single haze particles or a multi-haze particle mixing system, and the haze particle formation simulator has obvious value on treatment of the atmospheric environment.

The invention discloses an artificial intelligence stock investment analysis system which comprises a data receiving accelerator, a data storage unit, an investment strategy unit, an investment risk control unit, a quantitative transaction unit, a staring-in unit and an investment report unit. The data receiving accelerator is used for quickly acquiring the price and transaction data of the securities; the data storage unit is used for storing relevant data of securities; the investment strategy unit is used for setting an investment strategy and a strategy implementation period by a user; theinvestment risk control unit is used for analyzing historical market conditions and financial information of investment products and financial information of issuers to obtain investment risks of theissuers or the investment products; the quantitative transaction unit is used for automatically carrying out quantitative transaction according to an investment strategy of a user; the staring-in unit is used for acquiring and monitoring market change of securities in real time and giving an alarm according to a set threshold value; and the investment report unit generates an investment report according to the current stock and the transaction condition of the investment product.

The invention relates to the technical field of cell culture and particularly relates to a three-dimensional scaffold applied to cell culture and a preparation method thereof. Through the three-dimensional scaffold, a three-dimensional scaffold structure which meets the production requirement can be supplied to cell culture, the in-vivo culture environment can be simulated and the in-vitro culture effect of the cells can be improved. The three-dimensional scaffold applied to cell culture comprises a porous scaffold material; the content of water in the porous scaffold material is smaller than or equal to 1%; the porous scaffold material comprises collagen and chitosan.

The invention relates to a method for implementing strong vivo transplantation of human induced pluripotent stem cell-derived hemopoietic stem progenitor cells. The method is induced by an MLL-AF4 single factor and has the advantages that the method is simple, convenient and efficient, human iPS (induced pluripotent stem) cells can be induced into HSC (hemopoietic stem cells) with strong vivo transplantation effects and multi-strain hemopoietic reconstruction, and many difficulties in the field are broken through. The method mainly has the advantages that the number of factors needing to be added is greatly decreased by induction with the single factor, experimental operation can be remarkably simplified, experimental efficiency is improved, the risk of gene mutation possibly induced by slow virus mediated genome insertion is avoided by a plasmid-mediated inducing method, vivo transplantation efficiency reaches up to 20% or more, medullary system bias of iPSC-HSPC in vivo is corrected,and a medullary system, a lymphoid system and an erythroid can be comprehensively reconstructed in vivo.

The invention belongs to the technical field of animal model preparation, and in particular relates to a method for preparing a congenital microtia animal model. The method for preparing the congenital microtia animal model comprises the following operation steps: (1) feeding rats enabling to mate; (2) selecting pregnant female rats, and feeding in different cages randomly; (3) performing hypodermic injection of 30-60 mg / kg of retinoic acid to the female rats after 8-13.5 days of pregnancy; (4) normally feeding till 20 days of pregnancy, taking off the necks of the pregnant rats, and taking off embryos from deciduae, thereby obtaining the ongenital microtia animal model. The preparation method of the ongenital microtia animal model is simple to operate, low in cost, convenient to copy, good in repeatability and high in success rate, and compared with a transgenic congenital microtia animal model or a congenital microtia animal model prepared through gene knockout, the congenital microtia animal model is relatively close to the natural process of disease occurrence.

The invention relates to a biological specimen tomography cutting and imaging device and the working method thereof. The device comprises a material feeding module, a cutting module, a camera module, a controller and a computer wherein the cutting module and the camera module are both connected with the controller, the material feeding module is connected with the cutting module, and the control module is connected with the computer. The material feeding module is used to transport the biological specimen in need of cutting and controls the transportation speed; the cutting module is used to cut the biological specimen aided by infrared rays; the camera module is used to photograph the cut tomography of the biological specimen; the controller is used to control the size of the cut biological specimen, to control the camera module to photograph the cut specimen and to save the photographed images in a storage device; and the computer conducts three-dimensional reconstruction to the cut biological specimen. According to the method of the invention, through the technology of using the thinnest possible cutters, the biological specimen could be reconstructed in three dimensions by a computer so that a three-dimensional model for the biological specimen could be obtained accurately.

The invention discloses a preparation method of a high-quality bismuth selenide single crystal. According to the preparation method of the high-quality bismuth selenide single crystal, the molar ratioof Bi to Se is appropriately adjusted, a small excessive amount of Se is introduced as a transport agent, the problems that Se has high volatility and it is not easy to prepare a bismuth selenide compound with an ideal ratio are solved at the same time without introducing additional transport agents and fluxing agents, and the p-type or n-type high-quality bismuth selenide single crystal is prepared through an auto-doping effect. A bismuth selenide single crystal sample synthesized by the preparation method has high purity, an ideal stoichiometric ratio, a simple, environment-friendly and advanced synthesis process and high repeatability; and the synthesis product has a significant scientific value for studying the topological non-triviality of topological insulator materials.

The invention provides a random structural plane network contained rock mass model 3D printing and mechanical parameter acquisition method. The method comprises the steps of sampling a natural rock mass containing a random structural plane network, obtaining a three-dimensional space scanning image or a three-dimensional space distribution characteristic of the structural plane network, and obtaining mechanical parameter values of the structural plane network; and taking rock sampled at the same place of the natural rock mass for 3D printing to obtain a copy model, comparing mechanical parameter values of the copy model and the natural rock mass sample, adjusting a printing scheme until the copy model and the natural rock mass sample have similar mechanical properties, printing a plurality of copy models according to the adjusted printing scheme, carrying out various physical and mechanical tests, and obtaining mechanical parameters. According to the method, the natural rock mass structure can be accurately simulated, the mechanical properties of the rock mass containing the random structural plane network can be effectively researched, meanwhile, the random structural plane network of the rock mass can be repeatedly reproduced, multiple groups of tests can be conveniently carried out to obtain different mechanical parameters of the model, and the method has important significance on research on the mechanical property of the rock mass containing the random structural plane network.

The invention discloses a method for displaying and collecting distribution images of motor end plates inside levator ani muscles. The method comprises the following steps: dyeing a motor end plate inside a levator ani muscle sample by a cholinesterase mass dyeing process; preparing a hollow transparent silica gel model, and placing a background light source inside the silica gel model; placing the levator ani muscle sample with the dyed motor end plate on an outer surface of the silica gel model; acquiring the three-dimensional shape images distributed by the motor end plates inside the levator ani muscles; and placing the sample under a dissecting microscope so as to carry out microdissection and image acquisition. With the adoption of the method for displaying and collecting distribution images of motor end plates inside the levator ani muscles, the distribution images of the motor end plates inside levator ani muscles can be dyed, the purpose of completely displaying the distribution state of the motor end plates inside the levator ani muscles can be achieved under mapping of the background light source, and the effective research images for moving distribution rules of the distribution images of the motor end plates inside levator ani muscles are provided.

The invention discloses preparation and application methods of a mesoporous TiO2 surface enhanced raman scattering active substrate, relates to preparation and application methods of a surface enhanced raman scattering active substrate, and aims to solve the technical problem of relatively low activity caused by the existing technology of preparing the surface enhanced raman scattering active substrate from TiO2 nano particles. The method comprises the following steps: adding P123 into an ethyl alcohol, water and concentrated nitric acid mixed solution, thus obtaining a solution P123; dropping a tetrabutyl titanate solution into the solution P123 for stirring, thus obtaining sol; performing hydrothermal treatment and sintering on the sol, thus obtaining the mesoporous TiO2 surface enhanced raman scattering active substrate. The application is as follows: modifying the mesoporous TiO2 surface enhanced raman scattering active substrate with the surface of a detected object, and performing surface enhanced raman scattering test. The lowest detection concentration of the active substrate disclosed by the invention reaches 10<-8>M, so that the active substrate can be used for detecting medicine molecules.

The present invention provides a path planning method for an electric vehicle with a time window. The method comprises the steps of (1) setting relevant parameter variables, and establishing a model;2) designing a coding mode and redefining a related operation mode; 3) initializing a population, and initializing a central position and a vaccine; 4) grouping the population, executing a search modefor one part, turning to the step 5, executing a tracking mode for the other part, and turning to the step 6; 5) executing a search mode; 6) executing a tracking mode; 7) updating fitness of population individuals; 8) updating the central position of the population; 9) performing simulated annealing search on the population optimal solution, 10) finishing one iteration and updating the iterationfrequency g=g+1; 11) judging whether a termination condition is met or not, if so, executing the step 12), otherwise, returning to execute the step 4) and performing a new round of iteration, and 12)decoding the recorded optimal solution and outputting an optimal scheme.

The invention discloses a real-time fluorescence PCR identification method, primer and probe of a tiger derived component. The method comprises the following steps: 1, extraction of DNA from a sample; 2, real-time fluorescence PCR amplification detection; and 3, result determination. The primer comprises an upstream primer and a downstream primer, the sequence of the upstream primer is 5'-CTTTATCTGTCTATACATGCAT-3', and the sequence of the downstream primer is 5'-TGTAGGAGCCGTAGTATATT-3'; and the sequence of the probe is 5'-FAM-ACGAGGAATATACTACGGCT-Eclipse-3'. Compared with common PCR methods, the method disclosed in the invention has the advantages of simplicity in operation, and high efficiency and accuracy in identification, can effectively identify tiger night soil, tiger hair and tiger bones, and can be used for field tracing investigation and protection of tigers. The primer and the probe have very high specificity and sensitivity, and can be used for protecting and researching types of tigers.

The invention relates to a flexible layered mud sampler. The flexible layered mud sampler comprises a mud sampling tube which mainly comprises a first mud sampling tube, a second mud sampling tube and a third mud sampling tube, wherein the first mud sampling tube is hermetically arranged in the second mud sampling tube, the second mud sampling tube is hermetically arranged in the third mud sampling tube, the first mud sampling tube is provided with a piston, a first mud sampling opening is formed on the end part of the first mud sampling tube, a second mud sampling opening is formed on the end part of the second mud sampling tube, and a third mud sampling opening is formed on the end part of the third mud sampling tube. The mud sampler is simple in structure and can retract together in the carrying process, so that the mud sampler is convenient to carry. Moreover, when the mud sampler is used for sampling mud, the layering accuracy of a mud sample can be ensured, and composition and origin of the mud sample and biocenosis in the mud sample can be effectively researched.

The invention discloses an information sharing-based dangerous environment escape and rescue system. The system comprises a plurality of mobile terminals, a safety monitoring terminal and a cloud server, wherein the mobile terminals and the safety monitoring terminal are in two-way communication through a local area network, and the safety monitoring terminal and the cloud server are in two-way communication through the Internet; the mobile terminal comprises a voice talkback module, a gas detection module, a communication module, a positioning module and an anti-falling sensor module; and the safety monitoring terminal comprises a wind direction monitoring module and a wind speed monitoring module. According to the system, when the gas detection module detects dangerous gas, the voice talkback module in the mobile terminal is matched with the communication module and the positioning module to display alarm information sent by other mobile terminals and the safety monitoring terminal in the same area; the safety monitoring terminal transmits real-time wind direction and the wind speed to the mobile terminal through the wind direction monitoring module and the wind speed monitoring module via the local area network; and a purpose of timely escaping and rescuing the staff is achieved.

The invention provides a motor bus active rollover-prevention control test device and belongs to the field of motor bus active safety coefficient tests. The device is characterized in that digital signals of variables such as a bus speed, an acceleration, a suspension displacement are generated by a computer with a virtual motor bus system, and real sensor physical signals are simulated by a D / A conversion circuit through the digital signals, an active rollover-prevention control system collects the real sensor physical signals and makes an active rollover-prevention algorithm decision, the active rollover-prevention control system predicts whether the motor bus has a rollover danger at the next moment according to the driving state of the motor bus, if yes, a control signal of a suspension displacement adjusting device is output, the control signal is converted into a digital signal by an A / D conversion unit, and the digital signal is transmitted to the computer with the virtual motor bus system, so that the virtual execution mechanism is adjusted. By adopting the simulation platform, the active rollover-prevention technology is effectively researched, the risk of directly using an active rollover-prevention control system in a road test is avoided, and the test cost is lowered.

The invention provides a method for predicting an air entry value of a deformed soil based on a VG model, and can quickly and effectively predict the air entry value of the deformed soil. The method is characterized by comprising the following steps: step1, measuring a series of actually measured data of a measured matrix suction [psi]0 and a measured mass water content w0 under a deformation condition based on a pressure plate test; step2, substituting the series of actually measured data of the measured matrix suction [psi]0 and the measured mass water content w0 into the VG model, fitting asoil-water characteristic curve to obtain measured fitting parameters a0, m0, n0 based on the soil-water characteristic curve; and step3, substituting the measured fitting parameters a0, m0 and n0 into a prediction model for the air entry value of the deformation soil, and calculating the air entry value of the soil under the deformation condition, that is the predicted value.

The invention discloses a method for applying iTRAQ technology to study differentially expressed proteins of triple-negative breast cancer exosomes, comprising the following steps: (1) extracting and processing exosomes and exosome proteins; (2) performing step (1) The obtained exosomal protein sample is subjected to enzymatic hydrolysis and desalting to obtain a polypeptide; (3) iTRAQ labeling and mixing are performed on the polypeptide obtained in step (2); (4) HPLC separation and LC are performed on the mixture of polypeptide peptides labeled in step (3) ‑MS analysis; (5) Bioinformatics analysis and quantitative analysis were performed on the above-obtained mass spectrometry identification results to obtain differentially expressed proteins of triple-negative breast cancer exosomes. The iTRAQ technology provided by the invention combined with the MS / MS technology can quickly and effectively conduct the research on the exosome proteome of triple-negative breast cancer, and provide a reference for studying the disease mechanism of triple-negative breast cancer and discovering biomarkers related to triple-negative breast cancer.

The invention relates to a method for extracting high-purity lithium salt from lithium-containing brine, which belongs to the technical field of liquid-liquid extraction. The method comprises the following steps: after the extraction step and before the reverse extraction step, an exchanging step is introduced to exchange lithium and an impurity element, wherein an exchanging solution used in theexchanging step is a lithium salt solution. By virtue of the method of the invention, the high-purity lithium salt with the purity greater than 99.9 percent can be obtained, the reflux extraction theory is further applied to the lithium extraction of salt lake, an appropriate theoretical stage can be designed on purpose by virtue of calculation, the problems that the process is instable, the product purity is not high, the normal continuous production cannot be realized and the like can be solved, the scientific research and production for extracting the lithium from the brine can be effectively instructed, and the application prospect is wide.

The invention discloses a hospital follow-up visit method and device based on multi-source data and terminal equipment, and aims to perform life state analysis and judgment on internal and external data of a hospital and obtain follow-up visit data according to the data obtained through judgment, so that human intervention is avoided, life states of a large number of patients can be automatically judged, and the follow-up visit efficiency is improved. The consumption of manpower in manual follow-up visit is greatly reduced, the follow-up visit efficiency is improved, and the output of follow-up visit results is greatly accelerated. Compared with the prior art, by integrating medical data from multiple sources for automatic analysis, the precision and accuracy of follow-up visit data can be improved, scientific researchers can conduct effective research according to the obtained data, the medical level is further improved, meanwhile, direct contact with patients can be reduced, and the patient experience is improved. Therefore, the situation that the patient is disturbed due to follow-up visit is reduced, and the experience feeling of the patient is greatly improved.