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Method for preparing congenital microtia animal model

A technology for animal models and ear deformities, applied in medical preparations containing active ingredients, drug delivery, pharmaceutical formulations, etc., can solve the problems of difficulty in establishing animal models, affecting experimental research work, and low success rate, so as to achieve easy repetition and The effect of measurement and source is convenient and easy to obtain, and the cost is low

Inactive Publication Date: 2016-06-01
THE SECOND HOSPITAL OF SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The establishment of animal models of congenital microtia has become the key to the study of the pathogenesis, early prevention and early diagnosis of microtia, but the existing animal models of congenital microtia are complicated to operate and have a very low success rate, which brings great challenges to the establishment of animal models. Many difficulties have seriously affected the follow-up experimental research work

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  • Method for preparing congenital microtia animal model
  • Method for preparing congenital microtia animal model
  • Method for preparing congenital microtia animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for preparing an animal model of congenital microtia, using the following steps:

[0019] (1) Rat feeding and co-caging: 3-month-old SD rats were fed with pelleted feed, free to drink water during the feeding period, and the SD rats were caged overnight at a male-to-female ratio of 2:1. The vaginal suppository is defined as the 0th day of pregnancy, and at noon of the next day, it is determined as the 0.5 day of pregnancy;

[0020] (2) Selection step (1) Among the pregnant female mice, the female mice with smooth fur, good mental state and no signs of abortion were randomly divided into cages and raised;

[0021] (3) Retinoic acid intervention: Dissolve retinoic acid in olive oil and prepare a 40 mg / ml retinoic acid solution. On the 9th day of pregnancy, inject subcutaneously into pregnant female mice at a dose of 40 mg / kg body weight of retinoic acid;

[0022] (4) Continue routine feeding until the 20th day of pregnancy, kill the pregnant mice by dislocation,...

Embodiment 2

[0028] A method for preparing an animal model of congenital microtia, using the following steps:

[0029] (1) Rat feeding and co-caging: 2-month-old SD rats were fed with pelleted feed, free to drink water during the feeding period, and the SD rats were caged overnight at a male-to-female ratio of 2:1. The vaginal suppository is defined as the 0th day of pregnancy, and at noon of the next day, it is determined as the 0.5 day of pregnancy;

[0030] (2) Selection step (1) Among the pregnant female mice, the female mice with smooth fur, good mental state and no signs of abortion were randomly divided into cages and raised;

[0031] (3) Retinoic acid intervention: Dissolve retinoic acid in olive oil and prepare a 30 mg / ml retinoic acid solution. On the 8th day of pregnancy, inject subcutaneously into pregnant female mice at a dose of 30 mg / kg body weight of retinoic acid;

[0032] (4) Continue routine feeding until the 20th day of pregnancy, kill the pregnant mice by dislocation,...

Embodiment 3

[0038] A method for preparing an animal model of congenital microtia, using the following steps:

[0039] (1) Rat feeding and co-caging: 5-month-old SD rats were fed with pelleted feed, free to drink water during the feeding period, and the SD rats were caged overnight at a male-to-female ratio of 2:1. The vaginal suppository is defined as the 0th day of pregnancy, and at noon of the next day, it is determined as the 0.5 day of pregnancy;

[0040] (2) Selection step (1) Among the pregnant female mice, the female mice with smooth fur, good mental state and no signs of abortion were randomly divided into cages and raised;

[0041] (3) Retinoic acid intervention: Dissolve retinoic acid in olive oil and prepare a 40 mg / ml retinoic acid solution. On the 12th day of pregnancy, inject subcutaneously into pregnant female mice at a dose of 40 mg / kg body weight of retinoic acid;

[0042] (4) Continue routine feeding until the 20th day of pregnancy, kill the pregnant mice by dislocation...

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Abstract

The invention belongs to the technical field of animal model preparation, and in particular relates to a method for preparing a congenital microtia animal model. The method for preparing the congenital microtia animal model comprises the following operation steps: (1) feeding rats enabling to mate; (2) selecting pregnant female rats, and feeding in different cages randomly; (3) performing hypodermic injection of 30-60 mg / kg of retinoic acid to the female rats after 8-13.5 days of pregnancy; (4) normally feeding till 20 days of pregnancy, taking off the necks of the pregnant rats, and taking off embryos from deciduae, thereby obtaining the ongenital microtia animal model. The preparation method of the ongenital microtia animal model is simple to operate, low in cost, convenient to copy, good in repeatability and high in success rate, and compared with a transgenic congenital microtia animal model or a congenital microtia animal model prepared through gene knockout, the congenital microtia animal model is relatively close to the natural process of disease occurrence.

Description

(1) Technical field [0001] The invention belongs to the technical field of animal model preparation, in particular to a method for preparing an animal model of congenital microtia. (2) Background technology [0002] Congenital microtia is a deformity of the outer and middle ear caused by the abnormal development of the first and second branchial arches and the first branchial sulcus during the embryonic period. Many patients are also accompanied by dysplasia of the mandible and facial soft tissues on the same side. Congenital microtia is the most common facial deformity after lip and palate, and it is also the most common congenital deformity that causes facial asymmetry. [0003] Animal models are an important means to study the pathogenesis of teratogens. Reliable, stable, and easy-to-obtain animal models are helpful for the study of the pathogenesis, histological changes and molecular level of congenital malformations, and provide experiments for clinical prevention, diag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/203
CPCA61K9/0019A61K31/203
Inventor 刘磊
Owner THE SECOND HOSPITAL OF SHANDONG UNIV
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