43results about How to "Accurate introduction" patented technology

The invention discloses a method for site-specific mutagenesis of medicago sativa genes by employing a CRISPR / Cas9 system. First of all, a binary expression vector MsCRISPR / Cas9 is constructed; the vector is designed and constructed for a target gene; then, agrobacterium tumefaciens-mediated transformation is used to achieve site-specific mutagenesis of a specific gene, and a mutant transformed plant with the target gene knocked out is obtained by screening; the method uses the CRISPR / Cas9 technology for cross-pollination autotetraploid plant medicago sativa for the first time, and obtains a mutant plant; a MtU6 promoter is used for the first time to initiate sgRNA transcription in the medicago sativa, thereby improving the transcription efficiency; the mutation rate can reach 52%; the method realizes direct mutation on the coding sequence of the gene, and opens up a new field for gene editing in the medicago sativa, laying a technical foundation for simultaneous silencing of a plurality of genes, deletion of large chromosome fragments, precise insertion of foreign genes into targets, gene regulation and the like.

The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules (“binders”), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance site-specific integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders.

A dispensing appliance allowing the loading of a container together with a dispensing tube is disclosed. After loading the container into the holding portion of the appliance, a dispensing cartridge is engaged into a receiving system provided in the dispensing portion of the appliance, which is movable with respect to the holding portion from a first loading position to a second dispensing position. The cartridge partially encases a dispensing tube with both inlet and outlet ends protruding out of first and second sides of the cartridge, such that the outlet portion of the dispensing tube is flexible. Upon engaging the cartridge into the receiving system, the outlet portion is engaged into a pinch valve while the inlet points towards the dispensing opening of the container. The dispensing tube inlet is introduced into the dispensing opening upon moving the dispensing portion of the appliance to its second dispensing position.

The invention discloses a 3-D positioning apparatus and a method. The 3-D positioning apparatus comprises the components as following: an electric map storage portion, which stores 2-D position information and height information with multiple interpolating points; a map matching portion, which chooses at least one alternate path; a height detection portion, which calculates vehicle heights relative to multiple interpolating points; an error correction portion, which calculates the height of at least one alternate path; and a 3-D matching portion, which determines whether the vehicle height difference is less than a critical error, and the alternate path is chosen from the at least one alternate path with error less than the critical error for map match to the present position. The map matching precision on viaducts, underground roads, car parks and other roads having height difference with a flat road, the road is more accurately introduced, and much convenience is provided for a driver.

An apparatus and method for mixing and dispensing a powder material in an aqueous solution wherein the powder material is premixed into solution by a spreader. The premix solution is subsequently dispensed in a diluted aqueous stream by a venturi proportioner. In a preferred manner a hopper is provided for the powder material which is partially positioned in a mixing chamber. A valve and spreader are positioned at the base of the hopper.

The invention discloses a magnetic suspension rotor harmonic current inhibition method based on parallel FORC and phase lag-lead compensation, and the method comprises the steps: firstly building a magnetic suspension rotor dynamical model comprising quality imbalance and sensor harmonic; secondly employing a magnetic suspension rotor harmonic current inhibition method based on a parallel FORC structure. The structure enables internal models of a harmonic at all frequencies to be converted into a plurality of parallel units with the similar structures, and quickly improves the dynamic performances of a system. The method also employs the phase lag-lead compensation to improve the stability and dynamic performances of the system. The invention proposes a method for quickly and adaptively adjusting the controller gain for a specific harmonic signal. The method achieves the dynamic optimization of the dynamic performances and stability. The method achieves the inhibition of a harmonic component of a current of a magnetic bearing coil in a magnetic suspension rotor, and is suitable for the harmonic current inhibition of the magnetic suspension rotor system with the quality imbalance and sensor harmonic.

The invention provides improved systems, devices, and methods for analyzing a large number of sample compounds contained in standard multi-well microtiter plates or other array structures. The multi-well plates travel along a conveyor system to a test station having a microfluidic device. At the test station, each plate is removed from the conveyor and the wells of the multi-well plate are sequentially aligned with an input port of the microfluidic device. After at least a portion of each sample has been input into the microfluidic channel system, the plate is returned to the conveyor system. Pre and / or post testing stations may be disposed along the conveyor system, and the use of an X-Y-Z robotic arm and novel plate support bracket allows each of the samples in the wells to be input into the microfluidic network through a probe affixed to a microfluidic chip. A clamshell structure having a hinged lid can releasably support the chip while providing and / or accommodating the electrical, optical, structural, and other interface connections between the microfluidic device and the surrounding system.

The invention relates to a multifunctional tourist attraction service system with a team organization function and a realization method. Based on a communication function, a GPS (Global Position System) positioning function, a network connection function, a voice input and playing function, an information display and operation function and the like on an intelligent mobile terminal, the intelligent mobile terminal is connected with a network server and a processor to form the multifunctional tourist attraction service system with the team organization function; the service system is used for carrying out real-time dynamic monitoring on users and providing a series of service supports including supports on positioning and navigation of the users, tourist attraction information display and broadcasting and salvation services, so that a leader or a tour guide can conveniently manage and lead, and furthermore, the management efficiency and the communication efficiency are improved; and the service system has abundant tourism functions, can provide functions and services of accurate navigation, introduction, route recommending, reminding of lagging behind, warning of dangerous zones, tourist attraction congestion reminding and the like, and can bring relatively great convenience for the users in tourist attractions.

The invention discloses a magnetic suspension rotor odd harmonic vibration inhibition method based on a hybrid odd repetition controller. The magnetic suspension rotor odd harmonic vibration inhibition method comprises the following steps: firstly, establishing a magnetic suspension rotor kinetic model containing rotor mass unbalance and sensor harmonic; secondly, adopting the magnetic suspensionrotor odd harmonic vibration inhibition method based on the hybrid odd repetition controller (hereinafter referred to as HORC). The HORC is composed of an odd harmonic frequency inhibited second-orderinner film structure and a modified resonant controller (hereinafter referred to as MRSC), which are connected in parallel. The HORC can be used for improving a harmonic inhibition capability of a magnetic suspension rotor system when the harmonic frequency is perturbed, and the control robustness of a system under the harmonic frequency perturbation of the system is improved. According to the magnetic suspension rotor odd harmonic vibration inhibition method based on the hybrid odd repetition controller, an odd harmonic current component generated by a magnetic bearing coil in a magnetic suspension rotor is inhibited, so that odd harmonic vibration of the magnetic suspension rotor system with the rotor mass unbalance and the sensor harmonic is inhibited.

The invention relates to a dual-mode independent high-efficiency intelligent heat-dissipation timing switch panel. The dual-mode independent high-efficiency intelligent heat-dissipation timing switch panel comprises a circuit switch (1), a panel body (2), a pressing switch (3), a fan (9), a control module (4), and a time length input module (5), an electric control circuit breaker (6), a miniature electric control telescopic rod (7), a temperature sensor (8) and a fan speed regulating module (11) which are respectively connected with the control module (4). Based on the technical scheme provided by the invention, all the above structures are connected, and the dual-mode independent high-efficiency intelligent heat-dissipation timing switch panel is formed. According to the invention, a dual-mode independent control solution scheme is realized, any control process of people is intelligently and accurately responded, intelligent heat dissipation is realized for the heat generated in the working process of the designed control module (4), and the working efficiency of the dual-mode independent high-efficiency intelligent heat-dissipation timing switch panel is effectively ensured.

A conventional large-scale parallel pyrosequencing apparatus has the disadvantage that throughput decreases because much time and a large number of procedures are required for introduction of measurement beads, and analysis accuracy is deteriorated due to a reduction in accuracy of reagent replacement. There is provided an apparatus, wherein the apparatus includes a flow cell having a plurality of microfabricated reactors, and a camera opposed thereto, and DNA to be measured is fixed on the surfaces of beads having a specific gravity of 4 or greater, preferably zirconia beads. The flow cell is made horizontal when introducing the beads into the flow cell, and opposed to an optical axis of the camera when measuring an elongation reaction, the optical axis of the camera having a gradient with respect to the horizontal direction.

The invention discloses a sagger automatic continuous charging device for an electronic kiln, and belongs to the technical field of electronic kiln auxiliary facilities. The sagger automatic continuous charging device comprises a rack, a spiral material conveying tube, a material storage box and a power transmission mechanism. The rack is supported on a terrace. The spiral material conveying tube is fixed on the top of the rack, a screw rod shaft is matched in a tube cavity of the spiral material conveying tube, and a spiral sheet is formed on the screw rod shaft. A closed end is formed at the left end of the spiral material conveying tube, and an opened end is formed at the right end of the spiral material conveying tube. The material storage box is fixedly connected with the top of the rack, and the bottom of a material storage box cavity is communicated with the tube cavity of the spiral material conveying tube. The power transmission mechanism is connected with the screw rod shaft. The sagger automatic continuous charging device for the electronic kiln is characterized in that the opened end of the spiral material conveying tube is connected with a bend connector in a matched mode, the bend connector is communicated with the tube cavity, a bend connector discharging opening of the bend connector faces downwards, the left end of the screw rod shaft stretches out of the tube cavity to form a screw rod shaft extension shaft section, the screw rod shaft extension shaft section is supported on the top of the rack, and the power transmission mechanism is connected with the screw rod shaft extension shaft section. Discharging smoothness is guaranteed, the spiral sheet can be prevented from scraping the inner wall of the tube cavity, and accordingly materials can be accurately led into a sagger.

The invention discloses the technical field of liquid crystal display, in particular to a liquid crystal display panel. The liquid crystal display panel comprises a color filter substrate, an active switch array substrate, a liquid crystal layer and sealant; the color filter substrate comprises a display area and a non-display area around the display area, and the non-display area is provided witha second limiting part used for sealant limiting; the active switch array substrate is opposite to the color filter substrate; the liquid crystal layer is arranged between the active switch array substrate and the color filter substrate and located in the display area; the sealant is arranged between the active switch array substrate and the color filter substrate by surrounding the liquid crystal layer, and the sealant is located in the non-display area and arranged on the second limiting part. Accordingly, by arranging a sealant limiting part on a first substrate, the accuracy of sealant application can be improved.

A liquid injecting jig is provided. The liquid injecting jig includes a jig configuration including a plurality of parts adapted to be in cooperative engagement so as to position a channel within the jig configuration, wherein the jig configuration is adapted to fit an opening through which the channel is adapted to be received so as to expose the channel from the jig configuration. A jig set, a microchip case, and a microchip set are also provided.

The invention discloses a sagger automatic continuous charging device for an electronic kiln, and belongs to the technical field of electronic kiln auxiliary facilities. The sagger automatic continuous charging device comprises a rack, a spiral material conveying tube, a material storage box and a power transmission mechanism. The rack is supported on a terrace. The spiral material conveying tube is fixed on the top of the rack, a screw rod shaft is matched in a tube cavity of the spiral material conveying tube, and a spiral sheet is formed on the screw rod shaft. A closed end is formed at the left end of the spiral material conveying tube, and an opened end is formed at the right end of the spiral material conveying tube. The material storage box is fixedly connected with the top of the rack, and the bottom of a material storage box cavity is communicated with the tube cavity of the spiral material conveying tube. The power transmission mechanism is connected with the screw rod shaft. The sagger automatic continuous charging device for the electronic kiln is characterized in that the opened end of the spiral material conveying tube is connected with a bend connector in a matched mode, the bend connector is communicated with the tube cavity, a bend connector discharging opening of the bend connector faces downwards, the left end of the screw rod shaft stretches out of the tube cavity to form a screw rod shaft extension shaft section, the screw rod shaft extension shaft section is supported on the top of the rack, and the power transmission mechanism is connected with the screw rod shaft extension shaft section. Discharging smoothness is guaranteed, the spiral sheet can be prevented from scraping the inner wall of the tube cavity, and accordingly materials can be accurately led into a sagger.

The invention relates to the field of liquid crystal display technologies, in particular to a liquid crystal display panel which comprises a driving switch array substrate, a color optical filter substrate, a liquid crystal layer and frame adhesive. The driving switch array substrate comprises a display area and a non-display area, and the non-display area is arranged on the periphery of the display area and is further provided with a first spacing portion for spacing the frame adhesive; the color optical filter substrate is opposite to the driving switch array substrate; the liquid crystal layer is arranged between the driving switch array substrate and the color optical filter substrate and is positioned in the display area; the frame adhesive encircles the liquid crystal layer and is arranged between the driving switch array substrate and the color optical filter substrate; the frame adhesive is positioned in the non-display area and is arranged on the first spacing portion. The liquid crystal display panel has the advantages that frame adhesive spacing components are arranged on the first substrate, and accordingly the frame adhesive coating accuracy can be improved.

The invention discloses a lumen stent. The lumen stent comprises a tubular main body, an inner branch and an outer branch, wherein the inner branch and the outer branch communicate with the tubular main body respectively; the tubular main body comprises a first main body section, a taper section and a second main body section which are connected in sequence; the far end of the inner branch is connected to the taper section; the near end of the outer branch is connected to the taper section; and the far end of the inner branch is positioned above the near end of the outer branch. The lumen stent has the beneficial effects that the far end of the inner branch is positioned above the near end of the outer branch, so that when the far end of the inner branch is just released from a conveying sheath, the outer branch is still positioned in the conveying sheath, and blood flow can pass through the inner branch quickly, so that the impact of the blood flow on a stent system is reduced, positioning is facilitated, and a subsequent release process can be more stable.

The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules (“binders”), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance site-specific integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders.

A dispensing appliance allowing the loading of a container together with a dispensing tube is disclosed. After loading the container into the holding portion of the appliance, a dispensing cartridge is engaged into a receiving system provided in the dispensing portion of the appliance, which is movable with respect to the holding portion from a first loading position to a second dispensing position. The cartridge partially encases a dispensing tube with both inlet and outlet ends protruding out of first and second sides of the cartridge, such that the outlet portion of the dispensing tube is flexible. Upon engaging the cartridge into the receiving system, the outlet portion is engaged into a pinch valve while the inlet points towards the dispensing opening of the container. The dispensing tube inlet is introduced into the dispensing opening upon moving the dispensing portion of the appliance to its second dispensing position.

The invention relates to the technical field of liquid crystal displaying, in particular to a liquid crystal display panel. The liquid crystal display panel comprises a first substrate, a second substrate, a liquid crystal layer, spacers and a sealant, wherein the first substrate comprises a displaying area and a non-displaying area around the displaying area, and the non-displaying area is further provided with a first limiting part used for limiting the sealant; the second substrate is opposite to the first substrate; the liquid crystal layer is arranged between the first substrate and the second substrate, and is located in the displaying area; the spacers are arranged between the first substrate and the second substrate in a dispersing mode; the sealant surrounds the liquid crystal layer and is arranged between the first substrate and the second substrate; the sealant is located in the non-displaying area and is arranged on the first limiting part. By arranging a sealant limiting part on the first substrate, the accuracy of sealant coating can be improved.

The invention discloses a magnetic suspension rotor harmonic current inhibition method based on parallel FORC and phase lag-lead compensation, and the method comprises the steps: firstly building a magnetic suspension rotor dynamical model comprising quality imbalance and sensor harmonic; secondly employing a magnetic suspension rotor harmonic current inhibition method based on a parallel FORC structure. The structure enables internal models of a harmonic at all frequencies to be converted into a plurality of parallel units with the similar structures, and quickly improves the dynamic performances of a system. The method also employs the phase lag-lead compensation to improve the stability and dynamic performances of the system. The invention proposes a method for quickly and adaptively adjusting the controller gain for a specific harmonic signal. The method achieves the dynamic optimization of the dynamic performances and stability. The method achieves the inhibition of a harmonic component of a current of a magnetic bearing coil in a magnetic suspension rotor, and is suitable for the harmonic current inhibition of the magnetic suspension rotor system with the quality imbalance and sensor harmonic.

The invention discloses a three-dimensional locator and a method. The three-dimensional locator includes: an electronic map storage unit, which stores an electronic map including two-dimensional position information and height information of a plurality of interpolation points; a map matching unit, which selects at least one candidate road; The height detection unit calculates the relative height of the vehicle corresponding to the plurality of interpolation point units; the error correction unit calculates the at least one candidate road corresponding to the current position of the point between the previous interpolation point and the current position. The three-dimensional matching part confirms whether the difference between the relative heights of the vehicle is within the set critical error, and selects a candidate road within the critical error from the at least one candidate road to implement a map for the current position match. The present invention improves map matching accuracy on elevated roads, underground roads, construction parking lots and other roads with a height difference from flat roads, thereby introducing routes more accurately and bringing more convenience to drivers.

The invention discloses a method for preparing an accurate polymer network. The number of azide functional groups at the tail end of a polymer is controlled accurately by using a method combining reversible addition-fragmentation chain transfer (RAFT) and click chemistry, so that the number of crosslinking points is controlled and a regular polymer cross-linked network structure is prepared. In the method disclosed by the invention, gel in the polymer network is generated by performing a click reaction on two or more accurately-controlled functional end group azide or alkyne functional groups (E) at the tail end of a polymer chain and two or more end alkynyl or azide functional groups (E) via multiple steps of RAFT. The method has the advantages of accurate controllability of the number of crosslinking points and density of a polymer cross-linked network structure and suitability for various polymer network structures containing different functional groups.

The invention is suitable for the field of computers, and provides a commodity information management method. The method comprises the steps that position information of commodities on a shelf and parameter information of the commodities are input into a system in advance, when a consumer requires to display some commodities in a live broadcast process, the system actively or an anchor selects and calls the position information and the parameter information of the commodities and sends the information to a prompt end, and the anchor can see the parameter information of the commodities from the prompt end, so that the anchor can introduce the commodities more accurately; the anchor can accurately position the desired commodities on the shelf according to the position information, so that the time for searching a target commodity from a plurality of commodities is shortened, the anchor has more time for effective sales output in one live broadcast, the live broadcast effect is better, and the satisfaction degree of a commodity supplier or a consumer is improved.

The present invention relates to a tourist attraction multifunctional service system with grouping function and its realization method. It is based on the communication function, GPS positioning function, network connection function, voice input and playback function, information display operation function, etc. based on the intelligent mobile terminal. It is connected with the network server and the processor to form a multifunctional service system of tourist attractions with teaming function. The service system monitors the users dynamically in real time and provides a series of service supports, including positioning navigation and scenic spot information for users. Display and broadcast, support for rescue services, facilitate the management and leadership of team leaders or tour guides, improve management efficiency and communication efficiency, and have rich travel functions, including accurate navigation, introduction, route recommendation, straggler reminder, and warning of dangerous areas Functions and services such as scenic spot congestion reminders can bring greater convenience to users' scenic spot travel.