50 results about "Thelenota ananas" patented technology

The invention belongs to the technical field of food processing and in particular relates to a fast-puffed holothurian and a preparation method thereof. The method comprises the following steps: snipping a fresh holothurian at a position with the distance from the belly to one-third part of the anus of the fresh holothurian, heating the fresh holothurian for 15-25 minutes in hot water being 96-100 DEG after taking out the viscera, taking out and cleaning the treated holothurian for standby; placing the obtained holothurian in a high-pressure steam pot, heating for 3-8 minutes under the temperature being 105-115 DEG C, and drying under low temperature to obtain products. The holothurian is one selected from Dalian holothurian, cucumaria frondosa gunnerus and thelenota ananas; the low-temperature drying refers to vacuum freeze drying or low-temperature forced air drying. Before being eaten, the fast-puffed holothurian prepared by using the method disclosed by the invention is placed in a vacuum cup for puffing, and then can be eaten. The puffing of the puffed holothurian is soft, after rehydration, various sense organ qualities of the puffed holothurian are good, the carrying and the eating are convenient, and the function of nourishing in real time can be realized. The fast puffed holothurian is a novel holothurian product and makes up the blank in the market.

The invention discloses a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method for identifying seven sea cucumber species. The PCR-RFLP method includes the steps of firstly extracting DNA (deoxyribonucleic acid) modules of sea cucumbers; subjecting specificity fragment of a CO I (coenzyme I) gene to PCR amplification to obtain a fragment about 690bp; secondarily subjecting amplified product to restriction enzyme digestion; and finally subjecting the enzyme-digested product to electrophoresis detection. Different sea cucumbers obtain stripes in different numbers and sizes, and selected specificity incision enzymes respectively include: Californian paracanthurus cucumber: SSP I, North Atlantic Ocean cucumaria: EcoR V, apostichopus japonicas: SSP I, thelenota ananas: EcoR V, acaudina molpadioidea: Sca I, isostichopusbadionotus: Pvu II, and actinopyga mauritiana: EcoR V and SSP I. The PCR-RFLP method overcomes the defects that a conventional morphological identification method is prone to affecting in accuracy, is heavy in workload and long in consumption time and the like. The PCR-RFLP method can be completed by only one-time PCR reaction, one-time enzyme digestion reaction and one-time polypropylene gel electrophoresis, and is simple to operate, short in parting time and good in specificity.

The invention relates to a method for identifying apostichopus japonicus by means of a specific peptide fragment group, and a group of marker peptides capable of being used for identifying apostichopus japonicus when used alone or otherwise. The marker peptides are low-homology polypeptide fragments of apostichopus japonicus protein, wherein the low-homology polypeptide fragments are exclusive to apostichopus japonicus with a length of 5-30 amino acids. Even though it is found that there are plants or bacteria having the same protein, the marker peptides are exclusive to apostichopus japonicus by comparison with thelenota ananas, acaudina molpadioidea, isostichopus badionotus and holothuria Mexicana.

The invention discloses a Chinese medicinal composition for treating insomnia due to blood stasis. The Chinese medicinal composition comprises the following Chinese medicinal herbs: American ginseng, concha margaritifera, tuber fleeceflower stems, glossy privet fruits, black lotus seeds, heterophylly faalsestarwort roots, dongkey-hide galatin, tortoise plastron, common anemarrhena rhizome, deerhorn glue, largehead atractylodes rhizome, poria, rhizoma dioscoreae, dried longan pulp, ladybell roots, swimming crabs, axillary choerospondias fruits, tree peony root bark, cape jasmine fruits, common burreed rhizome, zedoary rhizome, spina date seeds, white paeony roots, herb of nutgrass galingale, largeflower jasmine flowers, epiphyllum oxypetalumca, szechuan lovage rhizome, fructus aurantii, thelenota ananas, platycodon roots, peach seeds, unprepared rehmannia roots, red paeony roots, medicinal cyathula roots, stigma croci, radix ophiopogonis, dendrobium, milkvetch roots, largehead atractylodes rhizome, largetrifoliolious bugbane rhizmome, radix codonopsis, Chinese angelica, radix bupleuri and radix glycyrrhizae preparata. Clinical experiments show that the Chinese medicinal composition can be used for safely and effectively treating insomnia due to blood stasis.

The present invention discloses a method for determining oligomerization thelenota ananas glycosaminoglycan content. The method is characterized by comprising: (1) respectively preparing an oligomerization thelenota ananas glycosaminoglycan sample solution and a fengshensu control sample solution; (2) adopting high performance liquid chromatography to analyze the oligomerization thelenota ananas glycosaminoglycan sample solution, wherein the chromatography conditions comprise that: the Shodex OhpakSB-804HQ type gel chromatography column is adopted, the mobile phase is 0.08-0.12 mol/L NaCl, the column temperature is 34-36 DEG C, the flow rate is 0.3-0.7 ml/min, the detection wavelength is 206+/-2 nm, and the injection volume is 20-30 mul; and (3) calculating the oligomerization thelenota ananas glycosaminoglycan content according to the high performance liquid chromatography analysis result.

A general saponin of Thelenota ananas, its preparing process, and its application in medicine field are disclosed.

The invention discloses an exclusive characteristic peptide segment and a method for identifying sea cucumber species by using the same. The exclusive characteristic peptide segment, namely a polypeptide marker, comprises particular characteristic peptide segments of sea cucumbers, apostichopus japonicus, California apostichopus japonicus, thelenota ananas, Atlantic cucumaria, holothuria atra andMexican ginsengs, and their sequences are shown in SEQ ID NO.1-13. According to the invention, the technology for identifying different sea cucumber species from at the polypeptide level is established, and the blank of sea cucumber species identification at home and abroad is filled.

The invention discloses a method for producing an instant asparagus product long in guarantee period. The method comprises the steps of cleaning asparagus, placing the asparagus in a blender to perform tumbling, soaking the asparagus in hot water at 85-95 DEG C, placing the asparagus into cold water to soak the asparagus, filtering the asparagus, drying the asparagus to cause the water content to be 35-45 wt%, and obtaining a first prefabricated material; adding water to a mixture of hawthorn, ficus carica, thelenota ananas and milk to perform decoction for 2-5 h, performing filtration, concentrating filtrate to cause the water content to be 15-35 wt%, and obtaining a second prefabricated material; placing the first prefabricated material in a boiled pot to perform braising for 20-35 min, adding salt, chicken essence and taro meal to perform uniform mixing and continuing to perform braising for 20-38 min, and obtaining a third prefabricated material; evenly mixing the second prefabricated material, the third prefabricated material, microcrystalline cellulose, calculus bovis extract, lotus leaf extract, yam flour, kiwi powder and ginkgo powder, performing bagging, performing vacuumizing and high temperature sterilization, and obtaining the instant asparagus product long in guarantee period.

The invention belongs to the field of extracting natural active substances by a biological technology and particularly relates to a method for extracting glycosaminoglycan from thelenota ananas. The method comprises the following steps of Step (1) pretreating the thelenota ananas, Step (2) conducting alkaline hydrolysis on a body wall of the thelenota ananas, Step (3) conducting double-enzymolysis on the body wall of the thelenota ananas, Step (4) using trichloroacetic acid to remove protein in a double-enzymolysis solution after finishing the enzymolysis, Step (5) using ethanol to precipitate glycosaminoglycan of the thelenota ananas, Step (6) drying raw glycosaminoglycan of the thelenota ananas, Step (7) using hydrogen peroxide to decolorize, Step (8) using a cellulose ion-exchange column chromatographic method to purify glycosaminoglycan of the thelenota ananas, and Step (9) drying refined glycosaminoglycan of the thelenota ananas. The method can balance a relation between yield and purity of glycosaminoglycan of the thelenota ananas, and improves the purity of glycosaminoglycan of the thelenota ananas.

The invention belongs to the field of extraction of biotechnology natural active materials and particularly relates to a method for extracting and purifying GAGs from thelenota ananas. The method includes: step one, pre-processing thelenota ananas; step two, performing alkaline hydrolysis on the body wall of the thelenota ananas; step three, performing two-enzyme enzymolysis on the body wall of the thelenota ananas; step four, removing proteins in two-enzyme enzymatic hydrolysate through trichloroacetic acid after the enzymolysis; step five, precipitating the GAGs of the thelenota ananas through ethanol; step six, drying GAGs raw sugar of the thelenota ananas; step seven, purifying the GAGs of the thelenota ananas by using a cellulose ion exchange column chromatography and a gel column sequentially; and step eight, drying GAGs refined sugar of the thelenota ananas. According to the method, the relation between the yield and the purity of the GAGs of the thelenota ananas can be balanced, and the purity of the GAGs of the thelenota ananas can be improved.

The invention relates to the pharmaceutical technical field, in particular to a compound of thelenota ananas total saponins extracted from thelenota ananas. The invention further provides a preparation method and a use of the thelenota ananas total saponins. The compound has low cost of extract of the thelenota ananas, strong controllability and stable quality.

The invention relates to the pharmaceutical technical field, particularly to an anti-cancer compound Ananaside C having a molecular formula of C41H63O17SNa and separated from thelenota ananas. The chemical structure and the spatial configuration of the compound are determined by various modern spectral analytic methods, particularly the comprehensive resolution of the advanced two-dimensional nuclear magnetic resonance spectroscopy and the high-resolution mass spectroscopy. A vitro anti-tumor experiment shows that the compound has the remarkable effect of inhibiting eight kinds of tumor cell strains such as A-549 lung caner, IA-9 ovary cancer and the like. The invention provides a precursor compound for developing a new anti-tumor pharmaceutical, and has the important value on the development and the utilization of the national marine medicinal organism resource.

The invention discloses a pipe fish kidney warming and yang invigorating sour soup base and a preparation method thereof. The soup base is prepared from the following raw materials in parts by weight: 45 to 55 parts of pipe fish, 20 to 30 parts of black-bone chicken, 20 to 30 parts of thelenota ananas, 15 to 20 parts of illicium verum, 15 to 20 parts of Chinese tuliptree bark, 15 to 20 parts of kidney of fur seal, 15 to 20 parts of cordyceps sinensis, 10 to 15 parts of deer's sinew, 10 to 15 parts of American ginseng, 10 to 15 parts of lucid ganoderma, 10 to 15 parts of dragon bone, 10 to 15 parts of cassia bark, 25 to 35 parts of wild tomato, 15 to 20 parts of spine date seed, 15 to 20 parts of raspberry, 15 to 20 parts of shine skin papaya, 15 to 20 parts of cornus officinalis, 10 to 15 parts of toosendan fruit, and 10 to 15 parts of rice wine. The preparation method comprises the following steps: preparing raw materials, extracting raw materials, carrying out centrifugation, mixing, preparing tomato paste, mixing, grinding, mixing all raw materials, concentrating, and drying. The provided soup base has a sour and fresh taste, is palatable, is rich in nutrients, and has the functions of warming the kidney, invigorating yang, dissipating binds, eliminating swelling, astringing, and inducing astringency, if people eat the soup base for a long term.