The invention relates to the technical field of
livestock and poultry breeding, in particular to a muscovy duck stress
rapid detection method and a muscovy duck
stress prevention and control method. The invention aims to solve the problems that when the Muscovy ducks are in stress reaction, the health of the Muscovy ducks is harmed, meanwhile, the meat quality of the Muscovy ducks is reduced, and a method for effectively, quickly and accurately detecting the stress reaction of the Muscovy ducks is lacked in the prior art. The
rapid detection method for the stress of the muscovy duck comprises the following specific steps: acquiring blood of the muscovy duck, extracting
RNA (Ribonucleic Acid), then carrying out reverse transcription on cDNA (
Complementary Deoxyribonucleic Acid), and detecting the expression quantity of Hsp70 through a fluorescent quantitative PCR (
Polymerase Chain Reaction) method. According to the present invention, the rapid and accurate detection of the Muscovy duck stress reaction is achieved, the
fluorescence quantitative PCR is adopted to detect the expression quantity of the Hsp70, the rapid and accurate Muscovy duck stress detection is achieved, the detection result can be obtained in the fastest 1 h, and the Muscovy duck can be accurately known through the method when the Muscovy duck is in the early stage of the body stress Muscovy duck, that is, the Muscovy duck is in the stress reaction when the expression quantity of the Hsp70 is higher than 0.3 ng / ml.