35 results about "Residual Tissues" patented technology

A fibrin injection mechanism for use in combination with an anastomosing and stapling attachment for an electromechanical device driver comprises a cylindrical dispensing chamber adjacent the cylindrical blade of the attachment and containing a sac filled with fibrin, a plurality of channels extending within the blade communicating between the dispensing chamber and the cutting edge of the blade, such that when the turning drive shaft is activated, the staple driver of the attachment moves forward to push a plurality of staples through corresponding staple ports and against the anvil of the attachment to pass through and staple together the target tissue, while the blade driver of the attachment moves forward to cut the unwanted residual tissue (post stapling), while a plunger (delivery) driver moves forward through the dispensing chamber to compress the sac to its break point, releasing the fluid medication into the dispensing chamber, and eventually pushing the medication though the channels to deliver the fibrin to the cutting edge of the blade and thus to the site of the newly stapled target tissue to accelerate the healing process.

The phacoemulsification needle according to the invention comprises a shaft, a tip disposed at a distal end of said shaft, the tip having a larger outer diameter than said shaft and an aspiration lumen extending through said shaft and said tip. The tip has an opening communicating with said aspiration lumen, a ball-shaped surface and a flat distal end comprising said opening. This needle can be used for removal of the cataract nucleus as well as for removal of the residual tissue.

The present invention provides systems and methods for selectively applying electrical energy to a target location within a patient's body, particularly including tissue in the spine. The present invention applies high frequency (RF) electrical energy to one or more electrode terminals in the presence of electrically conductive fluid to contract collagen fibers within the tissue structures. In one aspect of the invention, a system and method is provided for removing a vertebral disc in preparation for implanting a prosthetic disc or removing a portion of the vertebral disc such as the nucleus pulposus in preparation for placing a prosthetic nucleus within the annulus of the disc. The present invention also teaches shrinking residual tissue in preparation for placing the implants.

A device for removing undesired tissue such as residual tissue, epithelial cells and / or other undesired material(s) from an inner surface of a lens capsule of an eye, includes an elongated body [2] having a proximal and a distal end and at least one central lumen extending between the ends. A flexible shaving filament (4) is movably provided in the lumen so as to be insertable into the lens capsule. The filament has an overall stiffness such that it will be able to conform to an inner surface of a lens capsule when inserted into the capsule, and also to enable shaving off of material from the inner surface. The invention also relates to a method for removing the undesired tissue is also disclosed.

This invention relates to a method and apparatus for obtaining and registering the Electrical Cochlear Response (“ECR”). Particularly, the ECR refers to the electrical activity of the auditory nerve and the intracochlear residual tissue located in the neighborhood of an intracochlear stimulation electrode, when an external sound is presented to a Cochlear Implant user, human or animal. Applicants recognize that an ECR can be thought of as a far-field electrical potential registered on the scalp of an implanted patient. Applicants, through their method and apparatus, can extract that potential from the patient's spontaneous electroencephalographic activity (EEG) and measure it by using an averaging algorithm. The Electrical Cochlear Response can be applied for adaptation, calibration, performance evaluation and failure detection of the Cochlear Implant of the implanted patient. Also Applicants' method can be applied to estimate the audiometric thresholds of the Cochlear Implant even without the implanted patient's knowledge.

The present invention relates to a method for treating biological organic tissue, particularly plant tissue, by applying a direct current preferably of low voltage electrical field for short duration, with minimal detrimental influence on the treated tissue and to further beneficial uses of the residual tissue mass. The system and methods are useful for extraction and separation of substances of interest from the biological tissue, with optional concomitant reduction in enzyme activity, resulting in delayed or arrested browning of the tissue, and for production of imprint marks on the treated tissue. The present invention further relates to substances including pigments, minerals, oils, fragrances, vitamins, amino acids, alkaloids, extracted using the direct current treatment system, and to apparatus for performing the treatment.

The invention relates to amethod for efficientlyobtaining mesenchymal stem cells from umbilical cord tissues and aims at solving the problems of the long obtaining time and low efficiency of an existing method. The method comprises the steps that primary isolation of umbilical cord mesenchymal stem cells is performed: cleaned and cut umbilical cord tissue blocks are predigested by using trypsin and then digestive juice is removed, repeated flushing is performed with DPBS, then digestion is performed on a water bath shaker of 37 DEG C by using collagenase I digestive juice, a complete culture solution is added to the filtrate filtered by a cell strainer to terminate digestive centrifugal separation so as to obtain primary mesenchymal stem cell; residual tissue blocks are collected after filtration, and re-digestion is performed to obtain primary mesenchymal stem cells; the mesenchymal stem cells are inoculated in the complete culture solution, and standing and culture is performed in 5% CO2 culture box at the temperature of 37 DEG C; a culture solution is replaced for one time at intervals of several days; culture multiplication is performed: when the primary cells grow to be 80% and converge, TrypLE heavy suspension cells are subjected to culture multiplication in a serum-free culture solution. The method has the advantages of efficiently obtaining mesenchymal stem cells, and the obtained mesenchymal stem cells have highcell activity and differentiation potential.

The invention provides a multifunctional cutting-up bench for pathological diagnosis, comprising a support frame, a cutting-up bench surface, a functional frame, and a top plate, wherein the support frame is of a rectangle shape; the cutting-up bench surface is arranged on the upper surface of the support frame; the cutting-up bench surface is provided with the functional frame at one side with a long edge; the upper end of the functional frame is provided with the top plate; the lower end of the support frame is divided into a plurality of spaces by utilizing clapboards; a left space is of an apparatus room; apparatuses for sampling are placed in the apparatus room; a right space is of a storage room; the middle part of the functional frame is provided with a storage rack; the width of the storage rack is smaller than that of the cutting-up bench surface. The multifunctional cutting-up bench for pathological diagnosis provided by the invention is simple in structure, convenient to use, is capable of meeting multiple functions of cutting up, cleaning and disinfection of a work area, waste gas treatment, residual tissue smashing, cleaning and drying of hands, fixation and cutting up of medical bone tissues, and the like at the same time, greatly improves the work environment of pathologic cutting works, and improves the work efficiency of workers.

The invention provides a moldable specimen collection bag for a laparoscopic surgery, and belongs to the field of medical auxiliary instruments. The moldable specimen collection bag comprises a specimen bag and a steel wire, wherein a bag port in which the steel wire is arranged is formed in the top of the specimen bag. According to the moldable specimen collection bag, the flexible support of the steel wire brings a certain tensile force and relatively high moldability for the bag port of the specimen collection bag, and an operator operating a laparoscope can easily control the specimen collection bag, thus the time for moving out a separated tissue and organ specimen can be greatly reduced, and the residual tissue and organ can be effectively prevented from leaking. The moldable specimen collection bag is convenient for material selection, simple to manufacture, high in practicability, and worthy of popularization and application.

The invention relates to a bipolar radiofrequency intestinal tract anastomat and a working method. The anastomat comprises a near-end structure, a far-end structure and a radiofrequency energy generator, wherein the near-end structure is placed at the near end of an intestinal tract; the far-end structure is divided into a head portion and a handle portion, is located at the far end of the intestinal tract, and connects a positive pole with a negative pole of the radiofrequency energy generator through a guide wire inside the handle portion; and after the near-end structure is in butt joint with the far-end structure, the far-end structure becomes the negative pole, and a metal circular ring of the near-end structure is the positive pole. The intestinal tract is respectively sleeved on the near-end structure and the far-end structure, a portion needing anastomosis is collected to be attached to the metal circular ring of the near-end structure and a metal circular ring of the far-end structure, after sleeving, the metal circular ring of the near-end structure and the metal circular ring of the far-end structure form a circuit with the positive pole and the negative pole, intestinal tract anastomosis is completed after energization, after electricity is cut off, an internal push-type cutter is triggered through a switch of the handle portion, and residual tissue in the intestinal tract is cut off to guarantee smoothness of the intestinal tract. The anastomosis portion is continuous, operation time is short, no foreign matters are left in the intestinal tract after an operation, and safety of the anastomosis process is guaranteed.

The invention discloses a nose epithelial stem cell culture method which comprises the following steps: 1) laying nourishing layer cells into a culture dish; 2) separating primary cells of nose epithelial stem cells from nasal mucosa; 3) laying the primary cells of the nose epithelial stem cells on the nourishing layer cells, replacing a medium by a nose epithelial stem cell proliferation medium,and removing off residual tissue cells; 4) when the nose epithelial stem cells are proliferated to a density of 80%, carrying out trypsinization, and carrying out continuous passage amplification; 5)transferring the nose epithelial stem cells into a gas-liquid phase culture system and a PneumaCult-ALI differentiation medium, and carrying out differentiation culture. The invention further discloses a nose epithelial stem cell proliferation medium which comprises the following components: DMEM / F12, fetal calf serum, penicillin, streptomycin, insulin, an epidermal growth factor, hydrocortisone,3,3',5-triiodo-L-sodium adenoglycosine and an ROCK inhibitor. By adopting the method, a key application base is provided for transformation application of clinical transplanting of the nose epithelialstem cells.

The invention relates to a method for reproducing paraffin sections from kidney biopsy immunofluorescence frozen residual tissues, which comprises the following steps: quickly unfreezing the frozen residual kidney tissues by using a PBS buffer solution, dissolving OCT (Optical Coherence Tomography) gel, reproducing the paraffin sections, carrying out special dyeing by using the paraffin sections,and carrying out light microscope examination to obtain the paraffin sections toooooooo make up the condition that no glomerulus exists or the glomerulus is too little in the light microscope examination. The method has the advantages that the steps are simple, the operation is fast and convenient, the dyeing effect is good, the secondary kidney biopsy of the patient can be effectively avoided, and the pain and the economic burden of the patient are reduced.

The invention relates to the field of preparation of biological specimens and in particular to a method for preparing an otolith sample for early life history of fish. The method comprises the following steps: dripping a corrosive solution under a microscope to expose otolith; cleaning the exposed otolith for many times by using purified water, and removing the liquid and / or residual tissues obtained after cleaning; drying the otolith at room temperature, embedding the otolith by using a mounting medium, and drying after flatly laying the mounting agent; and performing grinding treatment before later otolith mounting in the early life history of fish. According to the method provided by the invention, the otolith obtaining difficulty in the stage of the early life history of fish is reduced, even if the sample tissues are not transparent, the method disclosed by the invention can also be used, and the prepared specimen is cleaner and fewer in impurities and contributes to long-term storage of specimens.

The invention relates to a cell culture medium change method, in particular to a neural stem cell medium change system and a medium change method for neural stem cell culture. The neural stem cell medium change system comprises a cell interceptor, a waste storage bottle, a sterile air bottle and an air pump in sequential connection, the cell interceptor is formed by connection of a titanium rod type filter head and a stainless steel pipe, and aperture of the titanium rod type filter is 4.0-5.0 micrometers. Alternatively, the neural stem cell medium change system structurally comprises the cell interceptor, a syringe and a connection pipe, the cell interceptor is made of the stainless steel pipe with a stainless steel filter screen inside, and mesh diameter of the stainless steel filter screen is 4.0-5.0 micrometers. The neural stem cell medium change system and the medium change method for neural stem cell culture have the advantages that medium change time in a neural stem cell culture process can be shortened, loss of precious neural stem cells in a medium change process is prevented, residual tissue fragments in a culture medium can be cleared away, lives of cells cultured in vitro are prolonged, and labor saving, productivity improvement and convenience for industrial production are realized.

The invention relates to a preparation method of an induced abortion pudendum cleaning antibacterial spray and a combined preparation. The invention aims at providing the preparation method of the induced abortion pudendum cleaning antibacterial spray with simple and convenient preparation, good stability, convenient use and good use effect and the combined preparation. According to the technicalscheme, the preparation method of the induced abortion pudendum cleaning antibacterial spray is characterized in that the induced abortion pudendum cleaning antibacterial spray comprises benzalkoniumchloride or benzalkonium bromide, chlorhexidine or chlorhexidine acetate or chlorhexidine dihydrochloride or chlorhexidine citrate or 20% of chlorhexidine digluconate solution, polysorbate 20, motherwort extract, shrubby sophora extract and collagen. The combined preparation provided by the invention is suitable for sterilization and antisepsis of vulva, vagina and residual tissues and repairing and antisepsis of uterus.

The invention discloses a method capable of enhancing the immunoregulation capacity of umbilical cord mesenchymal stem cells. The method comprises the following steps: (1) taking umbilical cord, carrying out washing, cutting the umbilical cord to be short, removing the amniotic membrane tissue on the peripheries of the blood vessel and the umbilical cord, cutting the residual tissue into small blocks, and carrying out culturing by adopting the StemPro MSC SFM CTS complete culture medium; and (2) when the cell merging rate achieves 25-30 %, carrying out digestion by adopting the pancreatin substitute CTS TrypLE Select Enzyme, carrying out washing, and collecting a cell suspension. For the mesenchymal stem cells treated by adopting the method provided by the invention, the secretion level ofthe immunoregulation related factors is high, the inhibiting effect for single karyocyte is great and is obviously higher than the effect of the conventional serum-containing culture medium, meanwhile, no influences are caused to the other properties of the mesenchymal stem cells, such as the differentiation capability, and thus the application prospect is good.

The invention relates to a traditional Chinese medicine pill for treating residual tissues in the uterine cavity after miscarriage, and belongs to the technical field of Chinese herbal medicine. The traditional Chinese medicine pill is prepared by the following steps: crushing peach kernel, safflower, angelica sinensis, ligusticum wallichii, radix paeoniae alba, radices trichosanthis, Chinese honey locust, spina gleditsiae, curcuma zedoary, rhizoma sparganii, leech, dianthus superbus, trogopterus dung, quispualis indica, seaweed, raw oyster, raw chlorite schist, leonurus, folium artemisiae argyi, radix aconiti lateralis preparata, medicinal cyathula root, achyranthes root and other traditional Chinese medicinal materials, and sieving powder by using a 100-mesh sieve. The traditional Chinese medicine pill has the functions of resolving hard lumps, breaking blood and eliminating aggregation, removing stasis and relieving pain, removing the fetus to benefit the mother and the like, can prompt continuous uterine contraction to remove blood clots, adhesions and the like in the uterine cavity, is good in curative effect, quick to become effective and convenient to take, is safe and reliable, has an effect of treating both symptoms and root causes, overcomes the shortcomings of existing medicine or technology for treating residual tissues in uterine cavity after miscarriage, is noninvasive, greatly relieves the pain of patients and has positive significance for treating residual tissues in uterine cavity after miscarriage.

The invention discloses a skin-grafting paste, which includes the following raw materials in parts by weight: 77-30 parts of pseudo-ginseng, 10-20 parts of pangolin, 6-20 parts of bitter galangal, 8-30 parts of dioscorea rhizome, and 10-20 parts of borneol, The preparation method includes: S1 weighing raw materials: weighing the raw materials of the skin-grafting paste in proportion by weight; S2obtaining medicine fine powder; S3 sieving; S4 mixing; S5 putting the finished skin-grafting paste into a sterilized bottle and performing sealing treatment . The skin-grafting paste prepared by the present invention is in close contact with the necrotic skin, penetrates the skin, removes rot, and decomposes the necrotic skin into a honeycomb shape, so that the leather-like necrotic tissue is automatically separated, the pain of necrotic tissue can be dissipated without a surgical excision operation, a necrotic skin layer can be eliminated without damage by liquefied pus-expelling, and the viable residual tissue and potential regenerative cells are preserved.

The split type anti-blocking tube drainage ball comprises a drainage ball body, an upper drainage tube, a drainage ball opening and a tee joint, the upper drainage tube and the drainage ball upper opening are arranged at the upper end of the drainage ball body, the tee joint is arranged at one end of the upper drainage tube, a movable clamp is arranged on one side of the upper drainage tube, and a lower drainage tube is arranged at the bottom end of the drainage ball body. And a filter screen is arranged in the drainage ball main body. The flow-out and the flow-out direction of drainage liquid can be controlled by adjusting the movable clamp and the valve on the tee joint, the drainage liquid can be extracted from the lower end of a wound of a patient, the lower drainage tube or the drainage ball main body for bacterial culture by adjusting the movable clamp and the valve, whether the wound of the patient is infected or not is identified, and the purpose of precise treatment is achieved. After the filter screen is additionally arranged, the filter screen can filter residual tissues and blood clots flowing out of the wound, the drainage tube opening is prevented from being blocked, operation of medical staff is facilitated, and the hospitalization cost of a patient is reduced.

The present invention is directed to an adult retinal cell line isolated from extra-retinal ocular tissue, and methods of isolating adult retinal cells from extra-retinal ocular tissue. The present invention is further directed to adult retinal stem cells isolated from vestigial tissue dissected from the eye of a donor mammal suffering from persistent fetal vasculature. The present invention is further directed to a culture medium for growing or maintaining retinal stem cells, and methods of maintaining adult retinal cells in culture. The present invention is further directed to methods of treating a treating an eye with retinal dystrophy using retinal stem cells, and an eye with glaucomatous injury with retinal stem cells. The present invention is further directed to kits for harvesting extra-retinal ocular tissue comprising a sterile container and a harvesting solution, wherein the kit allows the survival of the tissue until later dissociation of cells from the tissue.

The invention discloses a preparation method of autologous skin fibroblasts. The preparation method comprises the following steps: S1, cutting a piece of human autologous cheek or retroauricular skin tissue, completely soaking the tissue in a tissue treatment solution for 10-20 min in a sterile environment, removing adipose tissue and connective tissue from the soaked tissue, and cutting the residual tissue into pieces in a sterile culture dish; S2, adding the cut tissue into a mixed enzyme digestive juice preheated at 37 DEG C for digestion, and adding a digestion termination juice after digestion is ended to terminate digestion; S3, merging the obtained tissue fluid into a centrifugal tube, centrifuging, removing supernatant, and adding a cell culture fluid into the cell precipitate to obtain P0 fibroblasts; and S4, inoculating the P0-generation cells into a culture bottle, collecting the cells after culture is finished to obtain P1-generation fibroblasts, sampling, and counting the number and motility rate of the cells. The mixed digestive juice of multiple enzymes is adopted, digestion can be directly carried out, the operation process is simple, and consumed time is short.

The subject invention pertains to embodiments of a device for implantation into a residual amputated limb. More specifically, the subject invention provides one or more embodiments of an implantable force-distribution support that can be installed onto the terminal end of a bone or bone stump in a residual limb. The force-distribution support can restore the natural hydrodynamic system of the skeleton and can re-engage the axial skeleton as a factor in absorbing forces of ambulation. Embodiments can include a support bracket that can be attached to the terminal bone end to which a resilient-support can be attached to protect residual tissue from compression forces.

The invention relates to pesticide capable of preventing and treating seedling-period damping-off of chilies. The pesticide is prepared from the following components: mimoselike rosewood leaves, borax, ailanthus altissima leaves, sceptrocnide macrostachya maxim leaves, sodium chloride, washing powder, chicken bile, sodium hypochlorite and clean water. The pesticide disclosed by the invention is green and free from pollution, and pollution to the environment, and damages to human bodies, caused by the fact that a lot of chemical agents are used, are avoided; the half-year-cold mimoselike rosewood leaves, three-year-old to four-year-old ailanthus altissima leaves and two-year-old sceptrocnide macrostachya maxim leaves are treated by an enzymatic hydrolysis solution, mixed extract has an extremely great effect on inhibition of germination of pathogenic bacterium oospores, and is matched with a chicken bile, borax and washing powder mixture treated by sodium hypochlorite, so that the pesticide effect can be obviously improved, and sporangia and planoconidia, generated by diseased parts of young seedling, are killed; and the pesticide has inhibition and killing effects on mycelia which have saprogenesis in diseased residual tissues or humic matters, and can prevent a condition that the young seedlings are infected by sporangia and planoconidia, which are generated in the next spring. With the adoption of the pesticide, the prevention effect on the seedling-period damping-off of the chilies is up to 96.2% and the treatment effect is up to 96.1%.

The present invention provides a method for contrast-enhanced ultrasound imaging and a region detection and imaging method for contrast-enhanced images. The region detection method for contrast-enhanced images includes: acquiring tissue signals and contrast signals in the process of contrast-enhanced ultrasound imaging; Perform preprocessing respectively; compare the preprocessed tissue signal and contrast signal; segment the image formed after comparison processing into contrast agent action area, tissue residual action area and noise action area by means of threshold segmentation; contrast contrast image The corresponding positions on , are marked as contrast agent area, tissue residual area and noise area, respectively. On the one hand, the invention greatly improves the CTR of contrast-enhanced ultrasound images, which is beneficial to clinical observation; on the other hand, it enhances the signal of the contrast agent, so that the contrast dose injected during contrast-enhanced ultrasound can be greatly reduced, and the inspection cost is effectively reduced.

The invention relates to the field of preparation of biological specimens and in particular to a method for preparing an otolith sample for early life history of fish. The method comprises the following steps: dripping a corrosive solution under a microscope to expose otolith; cleaning the exposed otolith for many times by using purified water, and removing the liquid and / or residual tissues obtained after cleaning; drying the otolith at room temperature, embedding the otolith by using a mounting medium, and drying after flatly laying the mounting agent; and performing grinding treatment before later otolith mounting in the early life history of fish. According to the method provided by the invention, the otolith obtaining difficulty in the stage of the early life history of fish is reduced, even if the sample tissues are not transparent, the method disclosed by the invention can also be used, and the prepared specimen is cleaner and fewer in impurities and contributes to long-term storage of specimens.

The invention discloses a method for efficiently preparing an immature stem protoplast of asparagus officinalis related species asparagus filicinus. The method particularly comprises the steps that (1) 10-15cm long immature asparagus filicinus is selected and scaled; a top end part coated with scales is cut out; the immature asparagus filicinus is cut into 2-3cm small sections; skin is chipped by a blade; (2) the immature asparagus filicinus is lightly scrubbed for 10min in tap water, disinfected for 5min by 75% alcohol in a sterile operation platform, flushed for two times by sterile distilled water, disinfected for 10min by 10% sodium hypochlorite, flushed for four times by the sterile distilled water, and cut into 0.1-0.2cm sheets; 1.0g is weighed under the sterile condition, and placed in a 50mL sterile test tube; (3) an appropriate amount of enzymatic hydrolysate is added to the test tube; oscillation enzymolysis is performed at 100rpm on a light-proof shaking table at 25-28 DEG C; and (4) after the enzymolysis, the enzymatic hydrolysate is filtered; a residual tissue sample not enzymolyzed is removed; a filtrate containing the protoplast is obtained; and the purified protoplast is obtained by centrifugation, washing and the like.

The invention discloses a mandible inferior border posterior border elevator. The elevator comprises a strip connecting part, as well as a handheld part and an operating head which are fixedly connected with the two ends of the connecting part, wherein the operating head adopts a bent structure; a knife-edge structure with an arc transition surface on the outer side is arranged at the tip part of the operating head. The elevator adopts an anatomical shape which can be perfectly adhered to mandible, ensures that decollement operation is convenient, is suitable for the operation of an orthopedist, can properly peel mandible inferior border and posterior border, and greatly reduce residual tissue damage of a patient during operation.

The invention relates to a bipolar radiofrequency intestinal tract anastomat and a working method. The anastomat comprises a near-end structure, a far-end structure and a radiofrequency energy generator, wherein the near-end structure is placed at the near end of an intestinal tract; the far-end structure is divided into a head portion and a handle portion, is located at the far end of the intestinal tract, and connects a positive pole with a negative pole of the radiofrequency energy generator through a guide wire inside the handle portion; and after the near-end structure is in butt joint with the far-end structure, the far-end structure becomes the negative pole, and a metal circular ring of the near-end structure is the positive pole. The intestinal tract is respectively sleeved on the near-end structure and the far-end structure, a portion needing anastomosis is collected to be attached to the metal circular ring of the near-end structure and a metal circular ring of the far-end structure, after sleeving, the metal circular ring of the near-end structure and the metal circular ring of the far-end structure form a circuit with the positive pole and the negative pole, intestinal tract anastomosis is completed after energization, after electricity is cut off, an internal push-type cutter is triggered through a switch of the handle portion, and residual tissue in the intestinal tract is cut off to guarantee smoothness of the intestinal tract. The anastomosis portion is continuous, operation time is short, no foreign matters are left in the intestinal tract after an operation, and safety of the anastomosis process is guaranteed.