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Method for preparing otolith sample for early life history of fish

A sample preparation and life history technology, applied in the field of biological specimen preparation, can solve problems such as difficult long-term preservation, unclean specimens, difficulty in removing otoliths, etc., and achieve the effect of reducing difficulty

Inactive Publication Date: 2016-07-06
中国水产科学研究院北戴河中心实验站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a method for preparing otolith samples in the early life history of fish. The preparation method is easy to operate and can solve the difficulties in picking otoliths from fish in the early life history, the obtained specimens are not clean, The problem of not being easy to store for a long time

Method used

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  • Method for preparing otolith sample for early life history of fish
  • Method for preparing otolith sample for early life history of fish
  • Method for preparing otolith sample for early life history of fish

Examples

Experimental program
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Effect test

Embodiment 1

[0065] A method for preparing fish early life history otolith samples, comprising the following steps:

[0066] S11. Place a fish sample in an early life history under a dissecting mirror or a stereomicroscope, and drop a corrosive solution onto the fish sample to expose otoliths; the corrosive solution is weak acid, weak base or strong base salt solution;

[0067] S12. Clean the exposed otoliths with purified water for several times, and at the same time absorb the cleaned liquid and / or tissue residue;

[0068] S13. After the otoliths are dried at room temperature, embed the otoliths with a mounting agent, and dry the otoliths after they are flattened.

Embodiment 2

[0070] A method for preparing a transparent embryonic or larval otolith sample, comprising the following steps:

[0071] S21. Put the fresh yellow-covered plaice in the center of the glass slide, place it under a dissecting mirror or a stereo microscope, and adjust the magnification to an appropriate field of view.

[0072] S22. Acquisition and cleaning of otoliths

[0073] Add 5.5% sodium hypochlorite solution dropwise to the fish body to immerse the fish body in the solution. If the tissue dissolves too quickly, add purified water to dilute it appropriately, so as to dissolve the fish body tissue at an appropriate and controllable dissolution rate;

[0074] When the otoliths are clearly visible, wash them with purified water 3 to 4 times, and use filter paper to absorb the surrounding liquid each time. When the solution dissolves the otoliths and the area around them is clean and transparent, wash them with purified water, leaving the remaining fish body as a reference for ...

Embodiment 3

[0078] A method for preparing an opaque embryonic or larval otolith sample, comprising the following steps:

[0079] S31. Put the fresh sample of pufferfish larvae in the center of the glass slide, place it under a dissecting mirror or a stereo microscope, and adjust the magnification to an appropriate field of view.

[0080] S32. Acquisition and cleaning of otoliths

[0081] Add a sodium hypochlorite solution with a concentration of 6.5% to the fish body to immerse the fish body in the solution. If the tissue dissolves too quickly, add purified water to dilute it appropriately so as to dissolve the fish body tissue at an appropriate and controllable dissolution rate; Redfin pufferfish larvae in process such as figure 2 shown;

[0082] When the otoliths are clearly visible, wash them with purified water 3 to 4 times, and use filter paper to absorb the surrounding liquid each time. When the solution dissolves the otoliths and the area around them is clean and transparent, w...

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Abstract

The invention relates to the field of preparation of biological specimens and in particular to a method for preparing an otolith sample for early life history of fish. The method comprises the following steps: dripping a corrosive solution under a microscope to expose otolith; cleaning the exposed otolith for many times by using purified water, and removing the liquid and / or residual tissues obtained after cleaning; drying the otolith at room temperature, embedding the otolith by using a mounting medium, and drying after flatly laying the mounting agent; and performing grinding treatment before later otolith mounting in the early life history of fish. According to the method provided by the invention, the otolith obtaining difficulty in the stage of the early life history of fish is reduced, even if the sample tissues are not transparent, the method disclosed by the invention can also be used, and the prepared specimen is cleaner and fewer in impurities and contributes to long-term storage of specimens.

Description

technical field [0001] The invention relates to the field of biological specimen preparation, in particular to a method for preparing fish early life history otolith samples. Background technique [0002] Fish ecology research mainly includes age, growth, feeding habits, death and population changes. Since the change of fish population largely depends on the survival rate of early life history stages (embryo stage, larvae stage and juvenile stage), the ecological research of early life history is very important for the supplementation and population structure of adult populations of biological species. and population size are of great importance. [0003] Fish skeletal structures with solid anatomy such as scales, operculum, fin rays, vertebrae, etc., with chronological information and continuous growth throughout life can usually be used as a reference for fish age and growth status determination. The first calcified structures in bony fishes are usually otoliths, which a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/32G01N1/34G01N1/36
CPCG01N1/28G01N1/32G01N1/34G01N1/36
Inventor 任玉芹周勤王玉芬王青林司飞姜秀凤于清海张红涛
Owner 中国水产科学研究院北戴河中心实验站
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