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Method for reproducing paraffin sections from kidney biopsy immunofluorescence frozen residual tissues

A paraffin section and immunofluorescence technique, applied in the field of renal pathological examination, can solve problems such as affecting the work and accuracy of pathological diagnosis of renal biopsy, lack of glomeruli or a small number of glomeruli, and inability to formulate treatment plans for patients. The effect of secondary kidney biopsy, reducing pain and economic burden, and reducing cell damage

Pending Publication Date: 2020-11-03
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For light microscopy, kidney tissue needs to be made into paraffin sections. In actual work, the paraffin sections used for light microscopy may have no glomeruli or too few glomeruli, which directly affects the diagnostic work and accuracy of renal biopsy pathology , which in turn affects the progress of clinical work, and it is impossible to formulate an accurate treatment plan according to the exact pathological condition of the patient.
If a diagnosis is required, the patient must undergo a second kidney biopsy, which puts enormous pressure on the patient both mentally and financially

Method used

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  • Method for reproducing paraffin sections from kidney biopsy immunofluorescence frozen residual tissues
  • Method for reproducing paraffin sections from kidney biopsy immunofluorescence frozen residual tissues
  • Method for reproducing paraffin sections from kidney biopsy immunofluorescence frozen residual tissues

Examples

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Embodiment 1

[0060] In this example, paraffin sections were remade from immunofluorescent frozen remaining tissues of renal biopsies of patients with membranous nephropathy as an example. The glomerulus of membranous nephropathy was diffusely distributed, and antigen-antibody immune complexes were mainly deposited on the glomerular capillary wall. The changes under the light microscope were mainly lesions of glomerular basement membrane thickening. The specific operation steps are as follows:

[0061] A method for recreating a paraffin section of a renal biopsy immunofluorescent frozen remaining tissue, which includes the following steps:

[0062] Step 1: Kidney biopsy immunofluorescence frozen remaining tissue storage

[0063] Part of the renal tissue taken out by renal puncture, after being embedded in OCT gel, was promptly and quickly sliced ​​into frozen renal tissue with a frozen microtome for immunofluorescent staining, and the remaining unsectioned frozen renal tissue was quickly s...

Embodiment 2

[0098] Repeat Example 1, with the following differences:

[0099] In this example, taking the immunofluorescence frozen remaining tissues of patients with lupus nephritis to remake paraffin sections as an example, repeat the operation steps of Example 1, use the frozen remaining tissues of patients with lupus nephritis to remake paraffin sections, and seal the slides The paraffin sections were observed and photographed under an optical microscope. The lesions of lupus nephritis are more complex, and the light microscopic findings can be divided into types Ⅰ-Ⅴ based on pathological changes.

[0100] Such as figure 2 As shown, the four kinds of staining of the optical microscope photographs of the paraffin sections of frozen tissue produced in this embodiment fully meet the above-mentioned various staining requirements, and are consistent with the pathological changes of lupus nephritis shown by the contrast staining of conventional paraffin sections ( figure 2 -A with fig...

Embodiment 3

[0102] Repeat Example 1, with the following differences:

[0103] In this example, taking the immunofluorescence frozen remaining tissues of renal biopsies of patients with diabetic nephropathy to remake paraffin sections as an example, repeat the operation steps of Example 1, use the frozen remaining tissues of diabetic nephropathy patients to remake paraffin sections, and then seal the paraffin sections Observe and photograph under a light microscope. The lesions of each stage of diabetic nephropathy include hypertrophy of capillary loops, thickening of basement membrane, hyperplasia of mesangial matrix, K-W nodules, glomerular vitreous droplet lesions and glomerular fibrinous cap lesions.

[0104] Such as image 3 As shown, the four kinds of staining of the optical microscope photographs of the paraffin sections of frozen tissue produced in this example fully meet the above-mentioned various staining requirements, and are consistent with the pathological changes of diabeti...

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Abstract

The invention relates to a method for reproducing paraffin sections from kidney biopsy immunofluorescence frozen residual tissues, which comprises the following steps: quickly unfreezing the frozen residual kidney tissues by using a PBS buffer solution, dissolving OCT (Optical Coherence Tomography) gel, reproducing the paraffin sections, carrying out special dyeing by using the paraffin sections,and carrying out light microscope examination to obtain the paraffin sections toooooooo make up the condition that no glomerulus exists or the glomerulus is too little in the light microscope examination. The method has the advantages that the steps are simple, the operation is fast and convenient, the dyeing effect is good, the secondary kidney biopsy of the patient can be effectively avoided, and the pain and the economic burden of the patient are reduced.

Description

technical field [0001] The invention relates to the technical field of renal pathological examination, in particular to a method for reproducing paraffin sections by embedding frozen remaining tissues in renal biopsy immunofluorescent OCT glue. Background technique [0002] Kidney biopsy is the gold standard for the diagnosis of kidney disease. The full name is "renal biopsy", and it is usually called renal biopsy. Due to the wide variety of kidney diseases and the complex etiology and pathogenesis, the clinical manifestations of many kidney diseases are not completely consistent with the histological changes of the kidneys. In order to clarify the pathology of the disease and further diagnose the specific disease that the patient is suffering from, a renal biopsy is required at this time! The method of renal biopsy is to use a renal puncture needle to remove one or several small tissues from the kidney, and pass pathological examination to make a correct diagnosis of kidne...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N1/36
CPCG01N1/28G01N1/286G01N1/30G01N1/36G01N2001/2873
Inventor 周雅丽邢国兰
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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