The invention relates to a DNA library capable of diagnosing nystagmus infective genes through a targeted high-flux sequencing technique and an application of the DNA library. The library comprises 674 nystagmus infective genes. According to the DNA library disclosed by the invention, 674 nystagmus infective genes are preferably selected, a probe pond is designed, a target area library in accordance with 674 nystagmus infective genes is established, a high-flux sequencing technique is used by the library for sequencing, infective mutation is sought, and genetics and molecular biology basises are provided for clinical diagnosis. The DNA library disclosed by the invention has the characteristics of being accurate, quick, flexible and low in cost. The 674 gene related in the DNA library disclosed by the invention comprise infective genes of heredity diseases which nearly use nystagmus as clinical manifestation, including X-linked congenital nystagmus, oculocutaneous albinism, ataxia diseases, congenital amaurosis and cataract, and have important significance and clinical value on diagnosis, identification and diagnosis as well as pinpoint treatment of the nystagmus.