74 results about "Living sample" patented technology

A method is provided by which a reagent is provided to a living sample, including contacting a thin-film containing a porous body with a living sample containing cells or tissues, and discharging a solution containing at least one or more reagents to a non-contact side of the thin-film by ink-jet to provide the solution to the specific area of the living sample through pores on the thin-film, wherein the smaller diameter of the pores on the porous body relative to the diameter of cells in the living sample enables the reagent to be provided to each individual cell separately. According to this method, the reagent and the reaction product can be fixed while maintaining positioning information of the target substance in the living sample, as a pretreatment for analyzing with high positioning accuracy the target substance in each individual cell in the living sample.

A microscope includes a high humidity space that is maintained at a predetermined gas temperature and a predetermined relative humidity for culture of a living sample; an objective lens having a tip portion with a top lens which is disposed in the high humidity space, and serving to allow for an observation of the living cell incubated inside the high humidity space; a heater that heats the objective lens; and a temperature controller that controls the heater so that a surface temperature and an internal temperature of the objective lens are higher than a dew-point temperature and lower than a thermal death point of the living sample.

The invention discloses a rapid three-dimensional microscopic imaging system. The system includes a microscope, a field iris diaphragm, a narrow-band optical filter, a beam-splitting grating, a multiple focal-length lens array, a micro-lens array and an image sensor. The microscope amplifies a microscopic sample and images the microscopic sample to an image-plane extraction opening; the field iris diaphragm is used for restraining a post-imaging field range; the narrow-band optical filter is used for conducting narrow-band optical filtering on information from the sample; the beam-splitting grating is used for conducting beam splitting on microscopic output images at two-dimensional space, wherein the beam splitting is conducted after the microscopic output images go through a first-stage 4f system; the multiple focal-length lens array is used for adding different phase modulations into various light beams, so that images with different depths are obtained on a post-lens rear focal plane; the micro-lens array is used for modulating light rays at different angles to different special positions behind micro lenses, wherein the modulating is conducted after the light rays at the different angles go through the beam-splitting grating and the multiple focal-length lens array; the image sensor is used for recording sample images which are modulated by preceding stages. According to the system, the multi-depth synchronous imaging can be achieved at the single imaging speed of normal wide-field microscopes, the imaging is fast, the image resolution is high, and the system can be used for living samples; meanwhile, the system is simple in structure, and the imaging depth interval is convenient to adjust.

An automatic analyzer for performing qualitative and quantitative analyses of living samples, such as blood and urine, which effectively utilizes the sample and enables requested tests to be performed as many as possible, when sample deficiency is predicted as a result of measuring a sample volume in advance. The analyzer includes a unit for measuring a sample volume, and has a function of, when sample deficiency is predicted, automatically changing an analysis mode to a decrease sample assay for a part of tests, thereby reducing a sample volume required depending on the measured sample volume.

A fast fluorescence lifetime microscopic system images FRET between multiple labels in live cells and deep tissue, using a quantitative analysis method to reconstruct the molecular machinery behind the multiplexed FRET phenomenon. The system measures fluorescence lifetime, intensity and anisotropy as images of excitation-emission matrices (EEM) in real time and high speed within a single image scan, performs high-resolution deep-penetrating 3D FRET imaging in live samples, and fully analyzes all possible photon pathways of multiplexed FRET. The system provides a way for systematic and dynamic imaging of biochemical networks in cells, tissue and live animals, which will help to understand mechanisms of genetic disorders, cancers, and more.

In an automatic analyzer for performing qualitative and quantitative analyses of living samples, such as blood and urine, a capability of recognizing the analyzer state, e.g., the state under run of analysis or the stopped state, from a distance is provided without increasing the cost. To realize that capability, the screen saver function of a display is modified to have the function of recognizing the analyzer state and reflecting the recognized analyzer state on a design of a screen image displayed with the screen saver function.

PCT No. PCT / DE98 / 02954 Sec. 371 Date Aug. 4, 1999 Sec. 102(e) Date Aug. 4, 1999 PCT Filed Oct. 6, 1998 PCT Pub. No. WO99 / 22263 PCT Pub. Date May 6, 1999A microscope condenser with a condenser head, with the condenser being fastened to a microscope stand in a manner which allows it to move. A holding device for a glass container filled with a liquid is arranged above the condenser head. A living sample or cell for examination is arranged in the glass container. The microscope condenser in this case has a catch pan for liquid that has overflowed.

The invention discloses a transportation co-occurrence phenomenon visualized analysis method based on taxi trajectory data. The method comprises the steps that after basic data preprocessing is performed, region division and time division are performed on data, vehicles are mapped into different vehicle groups, then, the data is modeled in a graph structure, co-occurrence event data is extracted, analysis tasks are discussed on the basis of data characteristics and design principle, the visualized design flow is provided, various visualized methods are combined, a visualized design scheme containing three views is obtained, and the simple and clear expression mode is provided for co-occurrence analysis. The visualized design is analyzed and verified on the basis of Shanghai taxi trajectory data set living samples, so that intuitive analysis of the co-occurrence phenomenon is performed in a multi-angle and multi-level mode.

The invention provides a solution concentration detection method based on photothermal conversion nano-material temperature change. The method includes the steps of: 1) synthesizing a nano-material containing a photothermal conversion material to obtain a photothermal conversion nano-material; 2) using a series of standard concentration to-be-detected solutions to react with the photothermal conversion nano-material respectively so as to obtain a series of mixed solutions, and detecting the temperature change of the mixed solution at one time point under near-infrared light illumination respectively, and drawing a standard curve of temperature and concentration; and 3) completely according to the method of step 2), detecting the temperature change of an unknown to-be-detected solution at one time point, and performing comparison with the standard curve, thus obtaining the concentration of the to-be-detected solution. The method provided by the invention can realize fast, sensitive and portable quantitative detection on a variety of substances, like food, drugs, living samples and the like, and has the advantages of simple operation, good linear effect, low detection limit, low cost, portable instrument, and mobile detection, etc.

The invention relates to a field environment test system for small aquatic animals. The system comprises a support and culture boxes fixed on the support; the side faces of the culture boxes are made of a material with multiple holes, the tops and the bottoms of the culture boxes are made of a continuous material, and feeding guide pipes are arranged in the culture boxes. A method adopting the system for a test comprises the steps that the system is placed in a testing water area according to the test requirements, the height direction of the support is consistent with the water depth direction, and the small aquatic animals are cultured in the culture boxes; the small aquatic animals are fed through the feeding guide pipes, the behavior characteristics of the aquatic animals are recorded through photography devices, and the step is continuously performed for one test cycle; the survival number of the small aquatic animals is counted, living samples of the small aquatic animals are collected, quickly frozen by adopting liquid nitrogen and taken back, and the influence of a water body and sediment in the testing water area on the toxic effect of the small aquatic animals is detected. Compared with the prior art, the influence of the in-situ water body on the behavioral and physiological effects of the tested animals from the different time and space levels can be researched.

The invention discloses full-field optical blood flow velocity analysis equipment which comprises a vibration isolation optical table arranged in a darkroom and a customized container, a light source,an optical fiber coupler, an extension lens, diffusing glass, a support, a fixing frame, a high-speed complementary metal oxide semiconductor camera, a zoom double-center decentered lens and a computer which are arranged on the vibration isolation optical table. The customized container is used for storing biological samples; the light source is connected to the optical fiber coupler through optical fiber; according to the equipment, full-field optical angiography images of live samples are studied, and erythrocyte signals of background tissues are successfully highlighted; combined with image processing techniques such as fuzzy segmentation and skeleton extraction, blood vessels can be accurately segmented out from the full-field optical angiography images; the defect is overcome that laser speckles can only be used for measuring relative velocity of blood flow, and the defect is also overcome that the Doppler method can provide accurate transverse blood flow velocity measurement only in the direction of incident light.

A water body alga extinguishing purifier is realized through electrochemical reaction, the electrocatalysis and the electric field force action. When an electron flow passes through a living sample (alga), hydrogen bonds in the DNA molecules of the sample are broken so as to reach the alga extinguishing effect through gene mutation. Meanwhile, oxidation substances produced in the electrolyzed water such as HClO, H2O2, free hydroxy radicals, OH<-> and singlet oxygen O2, and active oxygen-derived free radicals have good effects on degrading organic pollutants in the water and extinguishing the alga in the water. The alga removal rate reaches 99.6 percent. Regarding the electrode structure, the purifier adopts a multilayer combined electrode. The purified is characterized in that the purifier can treat water through water without medicines; in addition, the purifier has high efficiency, long service life, convenient operating management, and the like. The purifier is suitable for the alga extinguishing purification of various water bodies.

The invention discloses a shore-based hermatypic coral spectral measurement method. A black adiaphanous cylinder is arranged, a water inlet is formed in the lower end of the cylinder, a water outlet is formed in the upper end of the cylinder, a black iron stand table is placed in the cylinder, sea water enters the cylinder through the water inlet and overflows through the water outlet, the sea water is kept flowing, then a hermatypic coral sample block is placed in the iron stand table in the cylinder containing the sea water, then a light source irradiates the hermatypic coral sample block, and a spectrograph is adopted for measuring the spectral reflectance of the hermatypic coral sample block; in the measuring process, a spectrograph probe is perpendicular to the surface of the hermatypic coral sample block, measurement is carried out many times, and averaging is carried out. Based on a shore-based laboratory, the wild measuring environment is simulated in the shore-based laboratory, and the spectral reflectance of hermatypic coral is measured. Therefore, a large amount of field observation time can be saved, measurement can be completed simply by taking back part of living samples in a hermatypic coral area, hard to reach, in a remote region, and the time and economic cost of field survey are greatly saved.

The present invention is based on the discovery that functional stratification and / or signaling profiles can be used for diagnosing disease status, determining drug resistance or sensitivity of cancer cells, monitoring a disease or responsiveness to a therapeutic agent, and / or predicting a therapeutic outcome for a subject. Provided herein are assays for diagnosis and / or prognosis of diseases in patients. Also provided are compositions and methods that evaluate the resistance or sensitivity of diseases to targeted therapeutic agents prior to initiation of the therapeutic regimen and to monitor the therapeutic effects of the therapeutic regimen. Also provided are methods for determining the difference between a basal level or state of a molecule in a sample and the level or state of the molecule after stimulation of a portion of the live sample with a modulator ex vivo, wherein the difference is expressed as a value which is indicative of the presence, absence or risk of having a disease. The methods of the invention may also be used for predicting the effect of an agent on the disease and monitoring the course of a subject's therapy.

The invention discloses a method for performing stepped detection on hydroxyl radical concentration of a solution. The method comprises the following steps: 1) modifying a nanometer material containing rare earth elements by using cyanine micromolecular compounds capable of reacting with hydroxyl radical to obtain a functionalized rare earth nanometer material; 2) performing reaction on a series of hydroxyl radical solutions with standard concentration and the functionalized rare earth nanometer material to obtain a series of mixed liquids, measuring the light intensity, the ultraviolet-visible light-absorbing intensity and the photo-thermal heating temperature of the mixed liquid separately, and drawing standard curves of the light intensity, the ultraviolet light-absorbing intensity, the heating temperature and the concentration separately; and 3) performing reaction on a to-be-detected radical solution with unknown concentration and the functionalized rare earth nanometer material to obtain a mixed liquid, determining the detection mode, measuring corresponding data and comparing with the standard curves to know the concentration. The detection limit is effectively reduced and the linear detection range is increased. The method is simple in material and low in instrument price, and can be applied to detection of samples such as food, medicines and living samples.

The invention discloses a cross-environment application deployment method, which comprises the following steps that: detecting whether a new material package used for releasing an application versionis in the presence in the first environment or not, and if the new material package used for releasing the application version is in the presence in the first environment, synchronizing the new material package to a second environment in the first environment; traversing the first subsystem of a started synchronous deployment switch in the first environment, and detecting whether the version of each application living example of the first subsystem is consistent or not; if the version of each application living example of the first subsystem is consistent, proving that the version is Vn, and calling the a first API (Application Program Interface) for detecting whether the version of each application living example of the second subsystem in the second environment is consistent or not; if parts of the version are not Vn, calling the second API in the second environment for the each application living example in the second system to release the version. The invention also discloses an operation and maintenance platform, an application deployment system and a computer readable storage medium. By use of the cross-environment application deployment method, the operation and maintenanceplatform, the application deployment system and the computer readable storage medium, the same system carries out the repeated operation of application living sample upgrade under different environments, and operation and maintenance efficiency is improved.

Described herein are micro-coil hyperpolarized NMR systems and methods for measuring metabolic flux in living and non-living samples. Such systems can perform high throughput measurements (with multiple coils) of metabolic flux without destroying the material, making it useful to analyze tumor biopsies, cancer stem cells, and the like. In certain embodiments, a hyperpolarized micromagnetic resonance spectrometer (HMRS), described herein, is used to achieve real-time, significantly more sensitive (e.g., 103-fold more sensitive) metabolic analyses of live cells or non-living samples. In this platform, a suspension mixed with hyperpolarized metabolites is loaded into a miniaturized detection coil (e.g., about 2 [mu]L), where the flux analysis can be completed within a minute without significant changes in viability. The sensitive and rapid analytical capability of the provided systems enables rapid assessment of metabolic changes by a given drug, which may direct therapeutic choices in patients.

The invention discloses a face living testing method, a medium and a related device, thereby improving accuracy of a face living testing result and safety of an identity identification system. The face living testing method comprises the steps of predicating a to-be-tested object by means of a pre-trained living predication model, wherein the living predication model is obtained through training sample data which are acquired by an infrared image acquisition device by means of at least one clustering algorithm, the sample data comprise living sample data and false sample data; and determiningwhether the to-be-tested object is a living object according to a predication result output from each living predication model.

The invention belongs to the technical field of luminescent probes, and discloses a luminescent probe suitable for time resolution and a time resolution fluorescence detection system matched with theluminescent probe. The luminescent probe is a nanoparticle with a particle size no more than 200 nm. The nanoparticle comprises a rare earth matrix, major doping elements and other optional doping elements. The major doping elements are one or more selected from Yb, Er, Tm, Nd and Ho, and the other doping elements are one or more selected from Sc, Y, La, Ce, Pr, Sm, Eu, Gd, Dy and Lu. According toa unique energy level structure of a rare earth material, after exciting of a certain energy level of the probe with suitable bands of short pulse lasers, and luminescence with the bands same as thebands of exciting light of the certain energy level is collected to achieve high signal-to-noise ratio and high sensitivity detection and imaging of biomolecules, cells, tissues or living samples.

The invention discloses a multifunctional living body sampling and analyzing device for a gastroenterology department. The sampling and analyzing device comprises a living body analysis box, a solid detection chamber and a liquid detection chamber are formed in the living body analysis box, a living body sampling device and a solid funnel are installed on the top of the living body analysis box, acentrifugal device and a solid analyzer are arranged at the bottom end of the solid funnel, the centrifugal device comprises a rotating disc fixed to the solid detection chamber through a transverseplate, a solid sample placing bottle is fixedly mounted on the top surface of the rotating disc, a liquid pump is arranged at the top of the liquid detection chamber, the bottom end of the liquid pumpis connected with a liquid sample transfer tube, a solution processing cylinder and a liquid analyzer are arranged below the liquid sample transfer tube, and a stirring device is inserted in the solution processing cylinder. The living body detection speed is fast, the digestive juice can be extracted, food or cell debris in the inner wall of a digestive tract can be scraped off, pathological cells or tissues can be clamped off, living samples are extracted from bodies of patients in multiple ways, and the sampling device is small and exquisite to alleviate the pain of the patients in the process of extracting the living samples from the patients.

The present invention relates to a non-thermal plasma jet device as spatial ionization source for ambient mass spectrometry of the type which allows a free adjustment of the geometry of the plasma beam, wherein the device comprises a double dielectric barrier probe of non-thermal plasma or NTP probe, which generates a non-thermal plasma jet; a high voltage and high frequency transformer circuit that connects an outer electrode through which it is possible to perform a discharge for the ionization of a gas which produces plasma, and wherein said plasma generator circuit is in turn connected to a source of AC power; an inner electrode which is grounded and allows to perform the discharge for the production of plasma; a storage tank of a gas serving as discharge gas; a test sample on which the non-thermal plasma jet is applied; and a ion transfer adapter to direct the ions produced by the device from the vacuum-free sample into a mass analyzer. The device described allows to directly analyzing live samples (plants, for example) without causing any damage to them.

The invention relates to the technical field of optical imaging. The invention provides a micro optical probe for solving technical problems such as that the existing micro-probe is overweight, oversized and cannot be used in clinical. The micro optical probe comprises a focusing lens for collecting nonlinear optics signals; a collimating lens for collimating laser light output by a laser input fiber and reducing chromatic aberration between lasers of different frequencies and outputting laser signals; a dichroic mirror scanner for separating the laser and the nonlinear optical signals and outputting the nonlinear optics signals, and for changing the angle of incidence of the laser to cause the laser to perform two-dimensional scanning on the plane of the internal tissue of living samples;an objective lens for concentrating laser light from the dichroic mirror scanner into a living body sample to excite the living sample to produce a nonlinear optical signal and for outputting a nonlinear optical signal.

The invention discloses application of a molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial in glutathione assay. The method comprises the following steps: a series of glutathione solutions with normal concentrations respectively react with the molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial, so that a series of mixed liquors are obtained. Under the irradiation of near-infrared light, the changes of fluorescence intensities are detected; after a standard curve of fluorescence and glutathione concentrations is drawn, glutathione solution with an unknown concentration reacts with the molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial, so that a mixed liquor is obtained; under the irradiation of near-infrared light, the change of fluorescence intensity is detected and compared with the standard curve, and thereby the concentration of the glutathione solution is obtained. The method can quickly, sensitively and portably carry out quantitative assay on the glutathione, such as the quantitative assay of food, medicines, living samples and the like, moreover, the method is easy to operate, the linear effect is good, the limit of detection is low, the cost is low, instruments are convenient to carry, and movable assay can be implemented.

The invention discloses a miniaturized self-adaptive optical two-photon fluorescent imaging system and a miniaturized self-adaptive optical two-photon fluorescent imaging method. The imaging system comprises a laser light source device, a miniature probe device, a wavefront detecting device, a de-scanning component and a fluorescent imaging device, wherein the laser light source device is used for outputting exciting light; the miniature probe device is used for receiving the exciting light output by the laser light source device, generating a fluorescent signal, and carrying out wavefront correction on various isoplanatic regions according to the average wavefront distortion distribution of various isoplanatic regions of a tissue plane imaging view field inside a living sample; the wavefront detecting device is connected with the miniature probe device for detecting the average wavefront distortion distribution; the de-scanning component is used for compensating the isoplanatic region off-axis effect generated by the miniature probe device in real time before the wavefront detecting device detects the average wavefront distortion distribution; the fluorescent imaging device is used for collecting the fluorescent signal output by the miniature probe device, and thus the imaging of the tissue plane inside the living sample is completed. With the system and the method, the large-visual-field, high-temporal-spatial-resolution and deep biological tissue imaging can be realized on an animal moving freely.

The invention provides a computer system used in determination of recurrence risk of colorectal cancer. The computer system is provided with a computer including a processor and a storage. The storage is recorded with a computer program which is executed in the computer. The method comprises receiving expression quantity of a plurality of genes selected by a first gene group existed in a region of 18q21 to 18q23 on an eighteenth chromosome long chain in a live sample acquired from a colorectal cancer patient, receiving expression quantity of a plurality of genes selected by a second gene group existed in a region of 20q11 to 20q13 on an twentieth chromosome long chain in a live sample acquired from a colorectal cancer patient, and receiving expression quantity of a plurality of genes selected by a third gene group including ANGPTL2, AXL, C1R, C1S, CALHM2, CTSK, DCN, EMP3, GREM1, ITGAV, KLHL5, MMP2, RAB34, SELM, SRGAP2P1, and VIM in a live sample acquired from a colorectal cancer patient, and based on the received expression quantity, determining recurrence risk of colorectal cancer for the patient.

An automatic analyzer for performing qualitative and quantitative analyses of living samples, such as blood and urine, which effectively utilizes the sample and enables requested tests to be performed as many as possible, when sample deficiency is predicted as a result of measuring a sample volume in advance. The analyzer includes a unit for measuring a sample volume, and has a function of, when sample deficiency is predicted, automatically changing an analysis mode to a decrease sample assay for a part of tests, thereby reducing a sample volume required depending on the measured sample volume.

The invention discloses a fish catcher which comprises an induction hole (1), a fish catcher attached cavity (2), a fish inlet (3), a fish catcher main body (4), a bait bottle (5), a fish catcher cover (6) and a lifting rope (7), wherein the induction hole (1) is in a cone structure and made of a transparent material, holes having the diameter being 2mm are distributed on the induction hole (1), and seven holes are arranged per square centimetre; the induction hole (1) is combined with the fish catcher main body (4) to form the fish catcher attached cavity (2); and the diameter D1 of the induction hole (1) is 96 millimetres, and the diameter D2 of the fish inlet (3) is 36 millimetres. The fish catcher disclosed by the invention aims at small juvenile fishes (having a length of 15-70 millimetres), and the purpose of catch is to observe and research the juvenile fishes as living samples. The fish catcher is small in size, convenient in use, immediate in catch while being used, suitable for various water bodies, and especially suitable for catch and research for fishes in forest mountain streams.

The invention relates to a surface-enhanced Raman scattering optical fiber probe, comprising an optical fiber which can be simultaneously used for transmitting exciting light and receiving Raman scattering spectrum, a tapered optical fiber and one quartz microballoon, wherein the tapered optical fiber is formed on one end of the optical fiber, and then one quartz microballoon is thermally welded on the tip of the tapered optical fiber; when the exciting light passes through the tapered optical fiber and the quartz microballoon, evanescent waves transmitted by the tapered optical fiber and thequartz microballoon can excite the Raman spectrum of a solution or a gas molecule to be tested; meanwhile, metal nano particles are coated on the optical fiber and the surface of the quartz microballoon, so that the Raman scattering strength can be enhanced. In addition, a sensing structure of the combination of the tapered optical fiber and the quartz microballoon also has the functions of reflecting, focusing and collecting the surface-enhanced Raman scattering spectrum. The optical fiber probe is combined with a monochromatic source and a high-sensitivity Raman spectrometer so that a Ramandetection sensing device can be formed. The invention has simple structure, strong capacity of resisting disturbance and high sensitivity, thereby being suitable for information acquisition and transmission of on-line analysis occasions, real-time detection occasions, live sample analysis occasions, trace hazardous and noxious substance measurement occasions and the like.

The present invention relates to a method for repairing subcutaneous tissue or dermal defects of patients. The method includes cultivating dermal fibroblasts from the patient living samples, cultivating umbilical cord mesenchymal stem cells of the same animal, mixing the two cells proportionally, and using the preparation essentially without immunogenic protein to repair subcutaneous or dermal tissues. The method can be used to repair wrinkles, skin expansion lines, pitting dense scar, and non-traumatic skin pit.