Method for performing stepped detection on hydroxyl radical concentration of solution

Abstract

The invention discloses a method for performing stepped detection on hydroxyl radical concentration of a solution. The method comprises the following steps: 1) modifying a nanometer material containing rare earth elements by using cyanine micromolecular compounds capable of reacting with hydroxyl radical to obtain a functionalized rare earth nanometer material; 2) performing reaction on a series of hydroxyl radical solutions with standard concentration and the functionalized rare earth nanometer material to obtain a series of mixed liquids, measuring the light intensity, the ultraviolet-visible light-absorbing intensity and the photo-thermal heating temperature of the mixed liquid separately, and drawing standard curves of the light intensity, the ultraviolet light-absorbing intensity, the heating temperature and the concentration separately; and 3) performing reaction on a to-be-detected radical solution with unknown concentration and the functionalized rare earth nanometer material to obtain a mixed liquid, determining the detection mode, measuring corresponding data and comparing with the standard curves to know the concentration. The detection limit is effectively reduced and the linear detection range is increased. The method is simple in material and low in instrument price, and can be applied to detection of samples such as food, medicines and living samples.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a method for detecting the concentration of hydroxyl radicals in a solution in a stepwise manner based on functionalized rare earth nanometer materials. Background technique [0002] Hydroxyl free radicals are small molecules with strong oxidative properties produced by the natural metabolism of organisms. Their oxidation-reduction potential is second only to fluorine, and they can affect a variety of biological processes. The abnormal content of hydroxyl radicals in organisms will increase the level of oxidative stress in organisms, and cause damage to single-stranded DNA, denaturation of proteins, etc. Therefore, the detection of hydroxyl radicals has practical significance. At present, common analytical methods include optical detection and electrochemical detection. However, the current common analysis methods have many defects, such as: slow dete...

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Problems solved by technology

However, there are many defects in the current common analysis methods, such as: slow detection speed, poor sensitivity, or incapable of accurate and effective quantitative detection, etc.

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