41 results about "Genus Escherichia" patented technology

There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has been modified to enhance an activity of aspartate-β-semialdehyde dehydrogenase.

L-Cysteine is produced by culturing a bacterium belonging to the genus Escherichia having an L-cysteine producing ability and modified so that cystathionine-β-lyase activity or cystathionine-β-lyase activity and tryptophanase activity should be reduced or eliminated in a medium to produce and accumulate L-cysteine in the medium and collecting the L-cysteine from the medium.

Amino acids such as threonine, homoserine, isoleucine, lysine, valine and tryptophan are produced using a bacterium belonging to the genus Escherichia which has been constructed from a sucrose non-assimilative strain belonging to the genus Escherichia and which harbors sucrose non-PTS or PTS genes and has an ability to produce the amino acid.

The invention discloses use of Escherichia coli S17-3 for the production of 2'-fucosyllactose. The preservation number of the Escherichia coli S17-3 is CCTCC 2018200. The Escherichia coli S17-3 with high yield of colanicacid is used for denovo synthesizing of 2'-fucosyllactose by using lactose as a sole substrate, and no synthetic route for exogenously expressing GDP-L-fucose exists. According toa fermentation method of recombinant escherichia coli for producing 2'-fucosyllactose, the fermentation output is increased by more than 10 times, the highest output reaches 617.0 mg / L, and the outputis greatly increased.

The invention provides methods for producing purine base analogues, purine nucleosides, and purine nucleotides, such as inosine and 5'-inosinic acid, which include using a bacterium belonging to the genus Bacillus or to the genus Escherichia wherein the purine productivity of said bacterium is enhanced by increasing an activity of the YdhL protein. The invention also provides the amino acid sequence of the YdhL protein from Bacillus amyloliquefaciens and the gene encoding it.

The present invention describes a method for producing L-threonine, which comprises the steps of: culturing a microorganism belonging to the genus Escherichia having the ability to produce L-threonine in an environment containing a carbon source, a nitrogen source, and a sulfur source fermentation medium, and collecting L-threonine, wherein the sulfur concentration in the medium is adjusted so as to be at a predetermined level or lower.

A method for comparing the variable reactivity of multiple, differentially mutated copies of 16S subsequences found in a number of ribosomal RNA operons of a single bacterial cell is described. The application of this method for distinguishing between closely related organisms, such as the genera Escherichia and Shigella, and between species of Shigella including S. boydii, S. dysenteriae, S. flexneri, and S. sonnei using nucleic acid probes is also presented.

The present invention relates to microorganisms of Escherichia coli having enhanced L-tryptophan productivity and to a method for producing L-tryptophan using the same. More particularly, the present invention relates to an Escherichia coli variant in which repression and attenuation control of the tryptophan operon is released and accumulation of anthranilate is reduced and thereby enhancing L-tryptophan productivity. The present invention also relates to a method for producing L-tryptophan using the Escherichia coli variant.

L-arginine, citrulline and pyrimidine derivatives including orotic acid, uridine, uridine 5'-monophosphate (UMP), cytidine and cytidine 5'-monophosphate (CMP) are produced using a bacterium belonging to the genus Escherichia harboring a mutant carbamoylphosphate synthetase in which the amino acid sequence corresponding to positions from 947 to 951 in a wild type carbamoylphosphate synthetase is replaced with any one of amino acid sequences of SEQ ID NOS: 1 to 9, and feedback inhibition by uridine 5'-monophosphate in the bacterium is desensitized.

There is provided a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, having the glycogen biosynthesis pathway disrupted.

The present invention relates to an Escherichia coli mutant strain having improved L-threonine productivity by the introduction of permease derived coryneform bacteria, and a method for producing L-threonine using the same.

The invention discloses healthy human intestinal escherichia coli and application thereof and relates to the field of microbial technology. The escherichia coli JL-3 belongs to Escherichia, and the preservation number is CGMCC No. 14074. The escherichia coli can remarkably prolong average lifetime of caenorhabditis elegans, can remarkably enhance heat-stress resistance of caenorhabditis elegans, can be settled in caenorhabditis elegans, has good gastrointestinal operation endurance ability, can generate nitric oxide signal molecule, and has a wide prospect of being developed into health food or medicines.

There is provided a method for producing L-threonine, L-valine, L-proline, L-leucine, L-methionine and L-arginine using bacterium belonging to the genus Escherichia wherein L-amino acid productivity of the bacterium is enhanced by enhancing an activity of proteins coded by b2682 and b2683 genes, or protein coded by b1242 or b3434 gene.

The invention represents a method for preparing amino acid L-threonine by using bacterium belonging to Escherichia genus. This bacterium shows ability for production of L-threonine and modified by so manner that expression of gene chosen from the group glk, pgi, pfkA, tpiA, gapA, pgk, eno and pykA encoding glycogenolysis enzyme is enhanced.

The present invention provides a method for producing L-amino acids or salts thereof by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to overexpress the yajL gene.

The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.

There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has L- theonine productivity and has been modified to enhance an activity of aspartate aminotransferase.

The present invention describes novel bacterial L-amino acids α-ligases, which catalyzing reaction of dipeptide formation having an acidic L-amino acid such as L-Asp or L-Glu at the N-terminus. The method for producing dipeptides using said L-amino acids α-ligases and a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain the DNA encoding said L-amino acids α-ligases, is described.

The invention discloses a modified Escherichia coli engineering bacterium and a method for producing citramalic acid by using the modified Escherichia coli engineering bacterium. According to the method, Escherichia coli S17-3 is taken as an original strain, and a T7 RNA polymerase gene for identifying a T7 promoter is inserted into a genome of the original strain, so that the modified escherichia coli engineering strain is obtained. The modified escherichia coli engineering bacteria further highly express citramalic acid synthase genes and one or more functional proteins of an inactivation auxiliary response factor RcsA, a response regulation factor RcsB, transmembrane sensing kinase RcsC, phosphate transporter RcsD and outer membrane lipoprotein RcsF. The invention provides novel industrial escherichia coli and a fermentation culture method, the strain is easy to culture, citramalic acid can be efficiently biosynthesized by using a cheap carbon source, and the production efficiency is higher than that of the existing biosynthesis method.

The present application discloses Zymomonas mobilis endogenous promoter mutants which guide the expression of heterologous nucleic acids that can be chimeric-linked, promote the expression of heterologous nucleic acids, and at the same time, these promoter mutants are high-expression promoters having a significantly enhanced function relative to wild-type promoters, the present invention relates to chimeric genes for expressing chimeric genes in cells of Zymomonas and / or Escherichia.

The invention provides methods for producing purine base analogues, purine nucleosides, and purine nucleotides, such as inosine and 5'-inosinic acid, which include using a bacterium belonging to the genus Bacillus or to the genus Escherichia wherein the purine productivity of said bacterium is enhanced by increasing an activity of the YdhL protein. The invention also provides the amino acid sequence of the YdhL protein from Bacillus amyloliquefaciens and the gene encoding it.

The present invention provides a method for producing L-amino acids by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the yjjK gene.

The present invention relates to an Escherichia coli mutant strain in which L-threonine productivity is improved by introducing a permease derived from Corynebacterium and a method for producing L-threonine using the Escherichia coli mutant strain.

There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has L- theonine productivity and has been modified to enhance an activity of aspartate aminotransferase.

A bacterium belonging to the genus Escherichia, which is transformed by introducing, into its cells, a DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine and a DNA coding for an aspartokinase III originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine; preferably a bacterium belonging to the genus Escherichia in which a dihydrodipicolinate reductase gene and a diaminopimelate dehydrogenase gene originating from Brevibacterium lactofermentum (or a succinyldiaminopimelate transaminase gene and a succinyldiaminopimelate deacylase gene) are further enhanced, is cultivated in an appropriate medium, L-lysine is produced and accumulated in a culture thereof, and L-lysine is collected from the culture.

The invention discloses an ammonium ion resistant colibacillus for producing succinic acid and application thereof and belongs to genus Escherichia. The colibacillus is named as Escherichia coli BER528, and the accession number CCTCC NO is of M2015160. On the anaerobic condition, the strain is capable of resisting high-density ammonium ions and accumulating succinic acid. During anaerobic shake flask fermentation with 0.53mol / LNH4 + added externally, 72h strain DCW is of 1.82g / L, the succinic acid yield is of 11.72g / L, but an original parent strain grows extremely slow, the 72h strain DCW is of 1.14g / L only, and the succinic acid yield is of 2.56g / L. Compared with that of the parent strain, the succinic acid yield is increased by 4.57 times. In the process of anaerobic fermentation with pH regulation through ammonia water by the aid of a 5L fermentation tank for 90h, the mutant strain DCW and final succinic acid yield are of 1.22g / L and 27.32g / L and is of 1.16 times and 1.88 times of those of the parent strain, and the mutant strain BER528 has important social benefit and economic value accordingly.

The invention discloses a method for producing colaric acid by acid-resistant high-density Escherichia coli. The E. coli species Escherichia coli S17-3 can be used to produce colaric acid, and the specific method is: adopt Escherichia coli species Escherichia coli S17-3 as the production strain, cultivate, and collect secretions, separate and obtain Cola acid. The preservation number of the Escherichia coli S17-3 species is: CCTCC 2018200. The present invention produces for the first time a mutant strain of Escherichia coli through acid-induced fermentation at 37°C, not only has high yield, but also has fast cell growth and short fermentation time, and provides a technical method for high-efficiency production of kolamic acid.

The present invention relates to a microorganism of the genus Escherichia in which L-tryptophan productivity is improved by inactivating phosphatase activity. Further, the present invention relates to a method for producing L-tryptophan using the microorganism of the genus Escherichia.

The invention discloses an acid-resistant high-density Escherichia coli and its application, the Escherichia coli species Escherichia coli The deposit number of S17-3 is: CCTCC 2018200. Escherichia coli species mentioned above Escherichia coli In the culture medium, S17‑3 can naturally extend the logarithmic growth phase of Escherichia coli without additional fermentation control, and can make the cell density OD during the overnight culture period 600 The value reaches more than 30, which is more than three times that of other Escherichia coli, and provides an important host cell for the economical and efficient high-density fermentation production of Escherichia coli.