The invention discloses an ammonium ion resistant colibacillus for producing succinic acid and application thereof and belongs to genus Escherichia. The colibacillus is named as Escherichia coli BER528, and the accession number CCTCC NO is of M2015160. On the anaerobic condition, the strain is capable of resisting high-density ammonium ions and accumulating succinic acid. During anaerobic shake flask fermentation with 0.53mol / LNH4 + added externally, 72h strain DCW is of 1.82g / L, the succinic acid yield is of 11.72g / L, but an original parent strain grows extremely slow, the 72h strain DCW is of 1.14g / L only, and the succinic acid yield is of 2.56g / L. Compared with that of the parent strain, the succinic acid yield is increased by 4.57 times. In the process of anaerobic fermentation with pH regulation through ammonia water by the aid of a 5L fermentation tank for 90h, the mutant strain DCW and final succinic acid yield are of 1.22g / L and 27.32g / L and is of 1.16 times and 1.88 times of those of the parent strain, and the mutant strain BER528 has important social benefit and economic value accordingly.