The invention discloses a method for producing colaric acid by acid-resistant high-density Escherichia coli. The E. coli species Escherichia coli S17-3 can be used to produce colaric acid, and the specific method is: adopt Escherichia coli species Escherichia coli S17-3 as the production strain, cultivate, and collect secretions, separate and obtain Cola acid. The preservation number of the Escherichia coli S17-3 species is: CCTCC 2018200. The present invention produces for the first time a mutant strain of Escherichia coli through acid-induced fermentation at 37°C, not only has high yield, but also has fast cell growth and short fermentation time, and provides a technical method for high-efficiency production of kolamic acid.