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Zymomonas mobilis endogenous promoter mutants

An endogenous promoter, Zymomonas technology, applied in the fields of synthetic biology and genetic engineering, can solve problems such as affecting downstream gene expression and changing promoter strength

Active Publication Date: 2022-07-22
HUBEI UNIV
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Problems solved by technology

[0003] However, with the increasing requirements for high-efficiency expression and precise regulation of target genes, the requirements for promoter strength and compatibility in different chassis cells are also increasing. For example, often when a host endogenous promoter is in the When used in another host, the strength of the promoter often changes, affecting the expression of downstream genes

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  • Zymomonas mobilis endogenous promoter mutants
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[0022] In order to make the purpose, technical solutions and advantages of the present application more clear, the present application will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application. The reagents not described in detail in this application are all conventional reagents and can be obtained from commercial sources; the methods not described in detail are all conventional experimental methods, which can be known from the prior art.

[0023] Described herein are novel promoters that can be used to express chimeric genes in host cells. The inventors of the present application creatively found that different types of mutants of the endogenous promoters of Z. mobilis: glyceraldehyde-3-phosphate dehydrogenase gene promoter and ZMO1609 gene promoter can improve the expression guided by the promote...

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Abstract

The present application discloses Zymomonas mobilis endogenous promoter mutants which guide the expression of heterologous nucleic acids that can be chimeric-linked, promote the expression of heterologous nucleic acids, and at the same time, these promoter mutants are high-expression promoters having a significantly enhanced function relative to wild-type promoters, the present invention relates to chimeric genes for expressing chimeric genes in cells of Zymomonas and / or Escherichia.

Description

technical field [0001] The present application relates to the technical fields of synthetic biology and genetic engineering, and in particular, to mutants of endogenous promoters of Zymomonas mobilis. Background technique [0002] Synthetic biology, as an emerging interdisciplinary subject, is booming and has a wide range of applications in agriculture, industry, smart materials and biomedicine. The basis of synthetic biology is biological elements. Sufficient and chassis-compatible biological elements (including promoters, terminators, ribosome binding sites, and riboswitches, etc.) The essential. As a core biological element, a promoter is a DNA sequence required for transcription initiation, usually located upstream of the 5' end of functional genes, and contains conserved sequences that specifically bind RNA polymerase and transcriptional regulators (such as regulatory proteins and small RNAs). The gene sequence of the promoter determines the expression intensity and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/70C12N15/74C12N15/65C12N15/53C12R1/01C12R1/19
CPCC12N9/0008C07K14/195C12N15/70C12N15/74C12N15/65C12N15/52Y02E50/10
Inventor 杨世辉宋皓月杨永富李涵杜军
Owner HUBEI UNIV
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