31 results about "Eremochloa ophiuroides" patented technology

The invention relates to a method for establishing an eremochloa ophiuroides highly efficient regeneration system with a young spike as an explant. The method includes the steps of explant selection and sterilization, callus induction and proliferation, callus differentiation, and rooting induction. The method provided by the invention is simple in explant sterilization, high in callus induction rate, easy to implement, and short in cycle of callus induction, obtained callus adventitious buds have high differentiation rate, test-tube plantlets are easy to root, and the method is suitable for eremochloa ophiuroides germplasm innovation, genetic inheritance transformation and other modern biological technology operations.

The invention provides an establishment method for a greening lawn mixed with flower spots and a roof flowered light lawn; the establishment method comprises the following steps: the production of a sod and a grass carpet which comprises the steps of turf bed laying, vegetative propagation or sowing propagation of eremochloa ophiuroides, secondary grass planting, over-seeding of low leguminous grass seeds, and the formation of grass carpet product; and verdurization of the lawn and the sod; in the latter step, leguminous grass seeds can be directly over-seeded on an eremochloa ophiuroides lawn; or the lawn is established by mixture sowing of the eremochloa ophiuroides and the leguminous grass seeds; or the eremochloa ophiuroides and the leguminous grass seeds mixed sowing sod is used forgreening lawn establishment, and the carpet-shaped eremochloa ophiuroides-leguminous grass carpet is used for the establishment of the greening lawn mixed with flower spots. The eremochloa ophiuroides -leguminous grass carpet can also be applied to roof lawn establishment, and the procedure comprises the steps of laying a waterproof layer, placing an anti-pricking layer, draining and irrigating water, laying and maintaining grass carpet. The establishment method of the invention has the advantages of simple production and establishment method, high quality of lawn establishment, good greeningdecorative effect, low cost, convenient maintenance and management, high economic benefit and extremely strong popularization.

The invention discloses the use of titanium with variable valence of light energy in improving the cold resistance of turfgrass, and discloses a method for improving the cold resistance of titanium with variable valence of light energy at the same time, comprising the following steps: Step 1, Preparation: Dilute the ionic titanium mother liquor containing light-energy variable-valent ion titanium with pure water to an ion-titanium solution with a concentration of ionic titanium of 8-10 mg / L; step 2, spraying of light-energy variable-valent ion titanium: adopt step 1 The prepared ionic titanium solution is sprayed for the first time on the turfgrass from the blooming stage to the fruiting stage, and the ionic titanium spraying treatment is carried out every 10 days. Apply three times to complete the cold resistance treatment. The present invention develops suitable spraying concentration and spraying method, improves the cold resistance of Wuling sage grass, promotes the prolongation of its green period, changes the state of using traditional reagents such as chemical fertilizers and growth regulators to prolong the state of sage grass, and promotes The turf industry is developing healthily and sustainably.

The invention relates to a centipede grass lateral bud inducting callus and plant regenerating method, which belongs to the technical field of the plant tissue culture. Lateral buds of a centipede grass are used as explantation material and vaccinated on callus inducting culture mediums of 2, 4-D-dichlorophe noxyacetic acid 1.0mg / L and BAP-6- benzyl aminopurine, and the inducting frequency of thecallus reaches more than 90%. The callus inducting culture mediums are transferred to a green seedling differentiation culture medium which is attached to a KT-sub- activator after being cultivated twice, and the green seedling differentiation reaches more than 12%. Lateral sprouts are used as explantation material, and yellow, compact and granular callus which is achieved is applicable to centipede grass micro-propagation, micro-propagation, somatic cell cultivation and agrobacterium mediating method gene conversion. The higher the employing quality of lawns is, the lower the quality of seedsis, and using lateral sprouts as explantation has special and important application value to establishment of grasses in lawns regeneration system, improvement of warm-season type grasses in lawns and industrialization of new species.

The invention provides a method for obtaining an eremochloa ophiuroide mutant, and belongs to the technical field of turfgrass biology. The method comprises the following steps of (1) obtaining mutated callus tissues; (2) performing differentiation culture on the mutated callus tissues to obtain induction seedlings; (3) inoculating the induction seedlings for performing rooting culture to obtain complete plants; (4) screening the complete plants to obtain variant plants; (5) performing cutting breeding on the variant plants; authenticating the mutant character; obtaining a stable and genetic eremochloa ophiuroide mutant. When the method is used, through once mutagenesis, 23 to 35 complete plants can be obtained; 5 to 10 complete plants generate variation on one or various characters; the character mutation rate is 21.73 to 28.57 percent; 2 to 3 plants generate DNA variation; the variation rate is 5.7 to 8.7 percent.