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Construction method and application of finger-print of centipedegrass germplasm

A construction method and technology of fingerprints, applied in the field of construction of fingerprints, can solve problems such as insufficient identification methods

Active Publication Date: 2019-03-26
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the problem of insufficient identification methods between different germplasm resources of C. chinensis, the present invention provides a method for constructing fingerprints of C. spp. , to protect local varieties, discover new germplasm, and make up for the shortcomings of traditional identification methods, and can use the most simplified markers for accurate, economical and rapid identification of wild and newly created germplasm

Method used

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  • Construction method and application of finger-print of centipedegrass germplasm
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  • Construction method and application of finger-print of centipedegrass germplasm

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Embodiment 1

[0090] 1. Selection of Germplasm Resources of Pseudomonas

[0091] The germplasm samples of C. chinensis came from 43 germplasm materials in different regions of my country and the United States, including 41 from China and 2 from the United States (E102, E158), as shown in Table 1.

[0092] Table 1 The core germplasm materials of Pseudomonas spp.

[0093]

[0094]

[0095] 2. Biochemical reagents

[0096] Polysaccharide and polyphenol plant total DNA extraction kit (EZgeneTM SuperFast Plant Leaves DNAKit, Biomiga); Mix for PCR amplification (2x TaqPCR MasterMix) and 100bp DNA ladder marker for gel electrophoresis analysis were purchased from Beijing Qingke Xinye Biotechnology Co., Ltd. Company; 9 pairs of SSR primers were purchased from the Shanghai Branch of Canadian Biotechnology Company.

[0097] 3. SSR primer sequence

[0098] According to the reported transcriptome information and related reports, 9 pairs of SSR primers were screened out, as shown in Table 2.

...

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Abstract

The invention provides a construction method and an application of a finger-print of centipedegrass germplasm and relates to the technical field of germplasm identification. The construction method comprises the following steps: first, extracting genomic DNA of centipedegrass samples from different germplasms separately; by taking the genomic DNA of centipedegrass samples as templates, carrying out PCR amplification on primers of one or more SSR molecular markers of (ACA)5, (CTG)11, (GAG)10, (CTG)12, (CCT)7, (CTC)11, (ATC)14, (CTC)10 and (CTAC)18; converting PCR amplification results of the samples into computer readable numerical codes to obtain the finger-print of centipedegrass germplasm. The construction method constructs the finger-print by selecting nine pairs of centipedegrass germplasm SSR molecular markers, so that centipedegrass from different origins can be differentiated simply and effectively, and the construction method is economical, practical and stable and reliable.

Description

technical field [0001] The invention relates to the technical field of germplasm identification, in particular to a method for constructing a fingerprint of the germplasm of Pseudomonas chinensis and its application. Background technique [0002] Pseudomonas belongs to the monocotyledoneae (Moncotyledoneae), Graminales, Gramineae, and Eremochloa in plant taxonomy. It is the only excellent warm-season lawn grass in this genus Type C4 herb. False Thriftgrass is mainly distributed in the Yangtze River Basin and the south of China, and the central and southern part of China is its natural distribution center. It is famous for its resistance to barrenness, few pests and diseases, and low maintenance level. It is recognized at home and abroad as the best lawn grass originating in China. , has the reputation of "Chinese lawn grass". As a native grass species, False Thrift Grass has good ornamental and application value, which is very in line with the current development trend of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6806
CPCC12Q1/6806C12Q1/6895C12Q2600/156
Inventor 李建建刘建秀宗俊勤汪毅郭海林陈静波李丹丹李玲王晶晶
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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