Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Eremochloa ophiuroides EoPHR2 gene and expression protein and application thereof

A kind of pseudo frugal grass, gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of low phosphorus mobility, high fixation, low effective phosphorus content and so on

Pending Publication Date: 2021-08-13
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low mobility and high immobilization of phosphorus in soil, the available phosphorus content that can actually be absorbed and utilized by plants is often low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Eremochloa ophiuroides EoPHR2 gene and expression protein and application thereof
  • Eremochloa ophiuroides EoPHR2 gene and expression protein and application thereof
  • Eremochloa ophiuroides EoPHR2 gene and expression protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Cloning of the EoPHR2 gene of Pseudomonas

[0027] Total RNA was extracted by RNAprep Pure polysaccharide polyphenol plant total RNA extraction kit (Tiangen). The reference sequence of the EoPHR2 gene was screened according to the transcriptome sequencing data (unpublished) of Pseudomonas. The SMART RACE kit from Dalian Bao Biological Co., Ltd. was selected for full-length gene cloning. The 3′RACE and 5′RACE primers involved are listed in Table 1. The amplified product was analyzed by electrophoresis on agarose gel, and the band with the correct size was excised, recovered and purified using Tiangen’s DNA gel recovery kit, connected to pMD19-T vector, transformed into E. coli Top10, and picked positive The clones were sequenced by a sequencing company (GenScript).

[0028] The obtained 3' and 5' fragments were compared and spliced ​​to obtain the full-length cDNA sequence of EoPHR2 gene. The ORF of the sequence was searched through the NCBI ORF Finder online prog...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an Eremochloa ophiuroides EoPHR2 gene and an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. Eremochloa ophiuroides adapting to low-phosphorus soil conditions is used as a material, the low-phosphorus response related EoPHR2 gene is obtained through homologous gene prediction and RACE technology cloning, the nucleotide sequence of the low-phosphorus response related EoPHR2 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the expressed protein is as shown in SEQ ID NO.2. A protoplast transient expression system is adopted to observe that the EoPHR2 protein has typical nuclear localization of transcription factors. A qRT-PCR method is utilized to analyze and find that the EoPHR2 gene has different expression modes under various tissue organs and stress conditions. Based on functional identification of arabidopsis thaliana transgenic plants, it is found that the transgenic plants grow better in a low-phosphorus environment compared with wild plants, and particularly fresh weight and the length and number of root hairs are higher.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and more specifically relates to a EoPHR2 gene and its expressed protein and application. Background technique [0002] Phosphorus, as an essential macronutrient for plants, is a structural component of nucleic acid in plant cells and cell membrane lipids, participates in various metabolic processes in plants, and plays an important role in plant growth and development. Although in most types of soils, including phosphorus-deficient acidic soils, the actual total content of phosphorus is not low. However, due to the characteristics of low mobility and high immobilization of phosphorus in soil, the content of available phosphorus that can actually be absorbed and utilized by plants is often low. Therefore, phosphorus, as an important factor limiting plant growth and development, is one of the most difficult nutrients to be absorbed and utilized by plants. Phosphorus uptake and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H5/06A01H6/20
CPCC07K14/415C12N15/8261
Inventor 王浩然李建建李晓慧姚祥赵新勇张苓王晶晶李玲
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com