64 results about "ELECTROPHORESIS INSTRUMENTATION" patented technology

The invention relates to a method for using a capillary electrophoresis instrument to detect the diffusion coefficients of a substance in a liquid phase. The invention discloses a plurality of key factors which affect the accuracy of the detection when using the capillary electrophoresis instrument to detect the diffusion coefficients of the substance, then further provides a fast detecting method which is used for the diffusion coefficients of the substance with a certain commonality and can detect the diffusion coefficients of the substance to be detected in water or an organic solvent. The method of the invention associates the diffusion coefficients of the substance with the appearance time of the elution peak of the substance as well as the width of a self-peak and can fast calculate and obtain the diffusion coefficient value of the substance.

An electrophoresis apparatus in which an electrophoretic channel formed in a planar plate made of a transparent member and having satisfactory flat and smooth surfaces is irradiated with an excitation beam through the bottom surface or the top surfaces of the channel in a direction orthogonal thereto, and fluorescence from a sample is detected through a side surface of the channel, or the channel is irradiated with the excitation beam through a side surface of the channel while fluorescence from the sample is detected through the bottom surface or the top surface of the channel. With this arrangement, background light and stray light can be reduced so as to enhance the accuracy of detection of the electrophoresis apparatus.

The invention is an improved multiplex capillary electrophoresis instrument or module with at least four and preferably six user-accessible vertically stacked drawers. An x-z stage moves samples from the user accessible drawers to the capillary array for analysis. An additional mechanical stage moves the array from side-to-side. The x-z stage, coupled with the additional array stage allows the system to sample all wells of a 384 well plate with a 96-capillary array. A computer program allows users to add capillary electrophoresis jobs to a queue corresponding to the analysis of rows or plates of samples without stopping or interrupting runs in progress.

A sample analysis method with improved separation accuracy is provided. The method relates to a method for analyzing a sample by electrophoresis using an electrophoresis apparatus provided with a channel and a sample reservoir formed in the channel. The method includes: placing the sample in the sample reservoir of the electrophoresis apparatus with the channel being filled with an electrophoresis running buffer; and performing electrophoresis by applying a voltage to both ends of the channel. The concentration of at least one of a) and b) is set to be approximately the same between the sample and the electrophoresis running buffer; wherein a) and b) are defined as follows:a) an ion that moves in the same direction as an analyte in the sample by the electrophoresis and has a smaller degree of mobility than the analyte, andb) an ion that moves in the opposite direction to the analyte.

The invention belongs to biomedical diagnosis, and discloses a method for detecting gonorrhea Neisseria by utilizing a loop-mediated isothermal amplification technology and a sequence of a primer used by the method. By combining the loop-mediated isothermal amplification technology with the fluorochrome development, the method can realize quick isothermal nucleic acid amplification of the specific DNA sequence of gonorrhea Neisseria and result detection on the premise of not using a PCR amplifier, an electrophoresis apparatus or an ultraviolet gel imaging system. The method is simple to operate, and can be used for detecting the gonorrhea Neisseria in urethral secretions of a clinic outpatient or serves as a method for self-check of patients.

The invention discloses a DNA quantitative method based on rolling circle amplification. The method comprises the following steps: performing rolling circle amplification reaction, restriction enzyme digestion reaction and electrophoresis on DNS standard solutions with different concentrations, and quantitatively analyzing the brightness of an objective strap to obtain a brightness value of the objective strap, thereby acquiring a standard curve between the concentration of the DNA standard solution and the brightness value of the objective strap; and then substituting the brightness value of the objective strap corresponding to the to-be-quantified DNA solution into a standard curve formula, thereby finishing the quantification of the to be quantified DNA. Through the adoption of the method disclosed by the invention, the sensitivity is close to the PicoGreen fluorescent dye quantitative method, an expensive device is unnecessary, and only a water bath pot, an electrophoresis apparatus and a plastic blooming instrument are required. The method disclosed by the invention can be used for quantitatively testing chain-typed DNA in a pg level.

The invention discloses a PCR (Polymerase Chain Reaction) detection primer and method of pseudomonas putida. The primer is that the nucleotide sequence includes a sequence 1 and a sequence 2. The detection method comprises the following steps of: 1) mixing the primer as disclosed in claim 1, a DNA template of bacterium solution to be detected, an Mg<2+> containing PCR buffer solution for PCR amplification, dNTP (diethyl-Nitrophenyl Thiophosphate) and Taq DNA polymerase to obtain a PCR reaction system; 2) transferring the PCR reaction system to a PCR instrument, and then amplifying according to the preset amplification condition; and 3) detecting an amplified segment through electrophoresis after the amplification is done so as to determine the structure. According to the detection method, the PCR method is carried out for detecting the gene segment of pseudomonas putida, thus the accuracy of the detection result is improved; the detection method is sensitive and only needs common PCR instrument and electrophoresis apparatus to reach the purpose of detection; and the detection result can be issued within 2 to 3 hours only.

The invention provides an integrated type electrophoresis apparatus. The integrated type electrophoresis apparatus comprises a power supply unit, a base, at least one electrophoresis tank, an electrophoresis tank tray and an upper cover which are integrated to form a whole body. The electrophoresis tank is directly arranged on the electrophoresis tank tray and a power supply can be switched on; the upper cover covers and then an electrophoresis experiment can be carried out, so that the integrated type electrophoresis apparatus is safe and convenient; the plurality of electrophoresis tanks can be arranged on the electrophoresis tank tray and are used for carrying out experiments simultaneously, so that instrument equipment and various connection circuits are reduced, a space is saved and a whole laboratory is more tidy; the integrated type electrophoresis apparatus further comprises a demisting and cooling circulating system; cooling air flow circulation is formed at front and rear parts in an electrophoresis apparatus box body, so that the inner temperature of the electrophoresis apparatus is kept in a reasonable range; the stability of an experiment result is improved and a condition that mist is formed on the upper cover, caused by the fact that heat is generated by electrophoresis, can be prevented, so that experiment personnel can observe conveniently.

The invention discloses an LAMP primer set for quickly detecting pseudomonas fluorescens producing thermostable lipase in raw milk and a method and belongs to the field of food safety inspection, andbelongs to the field of food safety detection. By taking a pseudomonas fluorescens lipase gene as a target gene, a set of specific detection primer set and an LAMP detection system and a reaction condition which are suitable for the primer set are designed and screened. A conventional detection method is long in time and consumes both manpower and physical materials. The adopted method is high insensitivity, strong in specificity and short in time; the cost is low, the operation process is simple, and a PCR instrument or an electrophoresis apparatus is not needed in the reaction process; andany instrument is not needed in the result judgment aspect. The shortcomings of the conventional method are overcome to a certain degree. The LAMP primer set and the method have significance on quickly detecting the quality of the raw milk. The adopted method is suitable for bed-layer on-line monitoring and field detection.

A method for analyzing a protein and a peptide, includes: providing a capillary for isoelectric focusing; providing a capillary device for separation and analysis having the capillary and a solid-phase extraction column being unified as a single tube-like structure; providing an electrophoresis instrument having the capillary device and the mechanism regulating the pressure difference at both ends of the capillary device; introducing a sample containing a target protein or peptide into the solid-phase extraction column to let the target protein or peptide be adsorbed on the column, and filling the capillary device with a carrier ampholyte solution; starting separation by isoelectric focusing after eluting the target protein or peptide by filling the solid-phase extraction column with electrode solution or acid or base solution, or after firstly eluting the target protein or peptide with an eluting solution containing carrier ampholyte and secondly filling the solid-phase extraction column with electrode solution or acid or base solution; and focusing the eluted target protein or peptide in the capillary for isoelectric focusing.

The invention relates to a method for using a capillary electrophoresis instrument to detect the diffusion coefficients of a substance in a liquid phase. The invention discloses a plurality of key factors which affect the accuracy of the detection when using the capillary electrophoresis instrument to detect the diffusion coefficients of the substance, then further provides a fast detecting methodwhich is used for the diffusion coefficients of the substance with a certain commonality and can detect the diffusion coefficients of the substance to be detected in water or an organic solvent. Themethod of the invention associates the diffusion coefficients of the substance with the appearance time of the elution peak of the substance as well as the width of a self-peak and can fast calculateand obtain the diffusion coefficient value of the substance.

The invention discloses an electrophoresis apparatus convenient for dropwise adding a test solution, which comprises an electrolysis box, a strip-shaped plate, a plastic bottle, a corrugated cylinder,a round box, a threading hole, a conductive wire, an electric plug, a strip-shaped hole, a column deep groove, an internal thread thin pipe, a thin screw, a motor, a sandwich plate, a sealing slidingstrip block, a connecting block, a U-shaped strip sliding seat, a conical dripper, a column hole block, a winding frame and a torsion spring. The electrophoresis apparatus is reasonable in design, can quickly perform electrolysis operation by only changing the variety of sample solutions and conveniently dropwise adding various sample solutions under the condition of ensuring sufficient other auxiliary sample solutions, achieves the effect of conveniently dropwise adding the sample solutions in a time-saving manner, is compact in structure, has the function of automatically loading and unloading the sandwich structure for electrolysis test, solves the problem that a traditional electrophoresis apparatus is complex in installation of a sandwich structure used for electrolytic testing, facilitates automatic winding of the detached conductive wire under the action of a torsion spring after operation is completed, and saves the arrangement time.

The invention provides a vertical electrophoresis tank and an electrophoresis apparatus. The vertical electrophoresis tank comprises a slide assembly, a gel frame assembly and a groove body assembly,wherein the slide assembly comprises a large slide and a small slide used for forming a gel preparing cavity, and a lateral opening groove communicated with the bottom of the gel preparing cavity is formed in the large slide; the gel frame assembly comprises a gel frame, a concave sealing member, a first negative electrode conduction member and a first positive electrode conduction member; the groove body assembly comprises a groove body, a pressing mechanism, a second negative electrode conduction member and a second positive electrode conduction member. The conduction of an electrode and thepressing of the slide assembly are synchronously completed by the pressing mechanism to eliminate the single operation of locking and inserting positive and negative electrodes sequentially in the slide assembly of traditional electrophoresis equipment to prevent the leakage of electricity and electric shocks; gel preparing and electrophoresis are completed in the vertical electrophoresis tank, additional gel preparing auxiliary devices are not needed, occupied space of electrophoresis testing equipment is saved, the operation is easier, and externally dripping gel liquid in the gel preparingprocess is accumulated in the groove body, so that the cleanliness of a testing tabletop is improved.

The application relates to the technical field of DNA automatic detection equipment, and specifically discloses a constant temperature device for a capillary electrophoresis instrument, which includes a heating base plate and a heating cover plate. The switch and the temperature controller are covered with a standing temperature plate on the heating bottom plate; the bottom of the heating cover plate is provided with a thermal insulation sponge. The purpose of this patent is to solve the problem of inconvenient replacement of the capillary of the existing capillary heating constant temperature device.