The invention relates to a biologically fermented composition with diabetes mellitus resistance. The composition is prepared by the method comprising the following steps: a, preparing of culture medium mixed liquid; b, sterilizing; c, cooling; d, inoculating to prepare fermenting broth; and e, standing to prepare the biologically fermented composition with diabetes mellitus resistance. The composition can be used for improving the micro-ecological environment in a body, contains saponin, amino acid, nucleic acid, trace elements and the like, and can be used for improving human body immunity, preventing diseases and effectively inhibiting blood glucose increase of patients with diabetes mellitus. The biologically fermented composition is especially suitable for patients with diabetes mellitus and obese groups, can be used for preparing pills, paste, aqueous preparations and other multiple preparations, and has an excellent assistant treatment effect on diabetes mellitus.