The invention relates to a method for depositing nanogold in a microfluidic pore passage. ITO glass provided with a (PDDA / PSS)n self-assembled multilayer membrane is prepared by carrying out pretreatment on ITO glass, the prepared ITO glass is taken as a substrate and is sealed with a chip made from PDMS to form a microfluidic chip, then mixed solution of KAuCl4 and H2SO4 is injected into the microfluidic pore passage, nanogold is deposited in the microfluidic pore passage by virtue of a chronoamperometry, and nanogold surface topography detection shows that uniformly distributed nanogold particles can be seen in the microfluidic pore passage. The method provided by the invention has the characteristics of rapidness, simplicity, good repeatability and low cost and has a broad application prospect in the fields of medical biology engineering, biomedical sensors and the like.