The invention belongs to the field of bio-
pharmacy, and particularly relates to a recombinant GLP-1 analogue
Fc fusion protein optimized
gene (rGLP-1-Fc for short) and an application thereof. The human GLP-1 analogue
Fc fusion gene is optimized, introduced into a
plasmid and transfected into CHOK1SV GS-KO cells, and after screening, a positive CHOK1SV GS-KO
cell line expressing GLP-1 analog
Fc fusion protein stably and efficiently is obtained. The constructed optimized
gene has the advantages that the
secretion and expression amount in positive
cell strains is increased significantly, the secreted
fusion protein has higher
biological activity and can directly act on a GLP-1
receptor, and affinity is higher;
blood sugar and glycosylated
hemoglobin can be reduced rapidly, efficiently and permanently; the optimized gene has functions of improving
islet beta cell function, reducing blood fat, reducing weight, delaying
gastric emptying, increasing satiety and the like.