The invention provides biological health padding and a preparation method thereof. The biological health padding includes: rice husk, sawdust, a compound microbial agent, dandelion, argy wormwood leaves, honeysuckle, mint, cogongrass, liquorice, isatis root and water. The padding is suitable for rural small-scale farming, helps to reduce livestock diseases, is beneficial to improvement of livestock and poultry antibody levels, prevention and control of animal digestive tract, respiratory tract and other diseases, and reduction of antibiotic drug residues in animal organism of livestock and poultry, and helps to promote the sustainable development of breeding industry.