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31results about How to "Save research money" patented technology

Multifunctional gas wave refrigerating jet stream field displaying apparatus and method for measuring

A kind of multiple functions gas wave cooling effluxion flow filed display device and measuring method which belongs to technique field of effluxion of pressure gas and expanded cooling and measuring control. The invention adopts integrated structure and works when running and static and possesses window for view and shooting image, it can analogue working mechanism and performance of multiple types of gas, and provides platform for leading high-precision PIV to wave mechanical flow filed measurement, and measures important parameters in-phase, and fulfills requirement of experiment, and accomplishes research content of changing condition, experiment component, rotate speed, two phases of gas and liquid, and accomplishes important experiment of best matching of structure and parameter. The structure is reasonable and easy, operation is convenient, and performance is stable and reliable. It fits for multiple functions gas wave cooling measuring filed of changing condition and research content.
Owner:DALIAN UNIV OF TECH

New drug against lung cancer and extraction separation method of new drug

The invention discloses a new drug against lung cancer and an extraction separation method of the new drug. The extraction separation method comprises the following steps of: performing homogenate, 70% alcohol extraction, deproteinization by trichloroacetic acid and vacuum freeze-drying on whole mollusk Vaginulus alteFerussac, performing chromatographic separation by gel columns repeatedly for multiple times, detecting molecular weight, molecular organization and primary molecular structures of the components, and conducting in-vivo and vitro anticancer pharmacology experiments to obtain an anti-lung-cancer active ingredient JK of the Vaginulus alteFerussac. Animal experiments prove that the heavy inhibition ratio of the JK on human-derived lung adenocarcinoma A549 cytoma reaches 54.37%; the heavy inhibition ratio of the JK on human-derived small cell lung cancer CNI-H446 cytoma reaches 59.73%; the heavy inhibition ratio of the JK on rat-derived Lewis lung cancer tumor reaches 49.49%; and the combination of the JK and cinobufotalin generates a synergistic effect. The experiments also prove that the JK has anticancer mechanisms shared by multiple Chinese traditional medicines, is compound with multiple target points, and relates to the synthesis of nucleic acid for inhibiting lung cancer cells, induction of lung cancer apoptosis, angiogenesis for restraining lung cancer tissue and the adhesion of the cancer cells on a vascular wall after the cancer cells invade blood circulation.
Owner:GUANGZHOU HANFANG PHARMA

Method for simulating verisimilar target echo

The invention discloses a method for simulating a verisimilar target echo, belonging to the field of electronic simulation. A plane wave database concept is derived from a multi-level subalgorithm of electromagnetic calculation, i.e. a scattered field of a target under incident wave excitation in any direction can be described by k plane waves, wherein k is related to the electrical size of the target. The invention expands the plane wave database concept, indicates K*K plane waves of the target under the excitation of an incident field in k directions according to a reciprocity principle and can accurately describe the scattered field generated by the excitation of the target in any incident direction and form a perfect broadband plane wave database N*K*K by N K*K databases under N different frequencies; and the scattered field of the target in any incident direction and frequency can be directly read by the broadband plane wave database or obtained through interpolation. The invention realizes the real-time simulation of the verisimilar target echo and can serve multiple radar application technologies, such as radar imaging, image features, target property, target identification, figuration design, and the like.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY

Multitudinous transplant method for woody flower group cultivated seedling

The invention relates to a planting method for culturing test-tube seedlings by plant tissues, in particular to the mass transplanting technique for tissue cultured seedlings of woody flowers. The invention adopts a container which is different from conventional culture flasks for one-time transplanting. The structure of the container used for transplanting is like a cup without a handle, and the bottom of the cup is provided with at least one hole, the mouth of the cup is provided with a cover, and the cover and the upper part of the container are transparent. The transplanting method using the container comprises the following steps that: substrates are prepared, the substrates are sterilized and filled in the container; the tissue cultured seedlings are sterilized; the tissue cultured seedlings are transplanted; the substrates in which the tissue cultured seedlings are planted are drenched by thimerosal; and the mouth of the container is sealed by the cover. Due to the adoption of the method, the seedling raising time is shortened, the transplanting survival rate can be kept at more than 90 percent, and the manufacturing cost is saved by 30 percent; moreover, the method is also wide in application.
Owner:HUNAN ACAD OF FORESTRY

Bridgehead settlement compensation amount test device and method

The invention discloses a bridgehead settlement compensation amount test device and method. The device comprises a pressurization unit, a model case and a control system. The pressurization unit comprises a frame, an oil cylinder, a pressure head and a guiding mechanism. The frame has a U-shaped structure and comprises a first support leg, a second support leg and a crossbeam. The oil cylinder is arranged in the middle of the crossbeam, the cylinder body of the oil cylinder is fixedly connected to the crossbeam, the end of a piston rod is provided with the pressure head and the guiding mechanism is arranged between the pressure head and the frame. The model case comprises a case body, a roadbed pavement model, a displacement sensor and several steel pipes. The roadbed pavement model is arranged in the case body. The upper part of the case body is provided with the displacement sensor. The several steel pipes go through the case body and are connected to the case body. The steel pipes are provided with water leakage apertures. Through simulation of different pavement structures, a relationship of a swelling amount increasing rule, compensation layer position, thickness, lateral confinement and compaction rate, and a lime slurry injection amount is researched and the relationship lays the foundation of design index formation.
Owner:SHANDONG JIAOTONG UNIV

Method and device used for monitoring and predicting scales in combustor

The invention provides a method and device used for monitoring and predicting scales in a combustor. The method comprises steps of by acquiring one kind of or various kinds of combustion signals of the combustor and / or a gas system corresponding to the combustor; determining combustor performance models corresponding to the combustion state signals according to the combustion signals; acquiring acombustor performance reference model corresponding to the combustor; and finally, according to the combustor performance model, by combining the combustor performance reference model, determining early warning information corresponding to the combustor. Thus, the generated combustor performance model is quite practical; by use of the method and the device, large quantity of research funds and usecost can be reduced; the scales and the development trend of the combustor can be monitored and predicted in a quite simple and convenient mode; and the method and the device can be used for strategyplanning of maintenance of economical combustors or gas turbines.
Owner:SIEMENS ENERGY GLOBAL GMBH & CO KG

Method for observing water level of multilayer underground water

InactiveCN111207806AWon't breakFacilitate popular installationMachines/enginesLevel indicatorsHydrometryObservational method
The invention discloses a method for observing the water level of multilayer underground water. The method comprises the following steps: S1, punching; S2, preparing a pipe; S3, observing a water level; S4, transmitting signals; S5, receiving the signals; S6, arranging data. A visual observation method is adopted, the water pump is used for pumping underground water at different heights for observation, and the height of the underground water level is visually observed. Equipment used in the method is ingenious, the equipment is not easy to damage, manufacturing cost is low, the underground water level observation device is durable in use, a large amount of equipment funds can be saved, popularization and installation of the water level observation device are facilitated, and scientific research funds are saved. The method can observe the underground water level in a circulating mode, cannot damage the underground water level, protects ecology, can observe the underground water level for a long time, collects data, and lays a data foundation for other hydrological scientific researches.
Owner:青海省环境地质勘查局 +2

Method for predicting inhibitory activity of fructose-1, 6-diphosphatase inhibitor based on quantitative structure-activity relationship model

The invention belongs to the technical field of drug activity prediction methods, and relates to a method for predicting the inhibitory activity of a fructose-1, 6-diphosphatase inhibitor based on a quantitative structure-activity relationship model. The method comprises the specific steps of collection of a sample set, processing and optimization of the sample set, establishment of an inhibitor molecule descriptor set, data set division, establishment of a QSAR linear model through a heuristic algorithm, establishment of a QSAR nonlinear model through a gradient boosting regression algorithm, and comparative analysis of two model results. In the initial development stage of the inhibitor, the molecular structure of the inhibitor is input through a computer, the inhibition concentration of the inhibitor is predicted based on HM and GBR methods, results of the two models are compared to solve the problem that prediction precision of a single linear model is not enough, the development risk of the inhibitor in the later stage is effectively reduced, and the research and development cost is reduced; the two established models are verified, so that the reliability of the models is proved; and meanwhile, a pioneer is created for application of the GBR algorithm in the field of computer-aided drug design.
Owner:QINGDAO UNIV

Method for calculating thermal conductivity of sandstone by utilizing the mineral components

According to the method for calculating the thermal conductivity of the sandstone by utilizing the mineral components, the thermal conductivity value of the sandstone is predicted and calculated by utilizing existing mineral data, so that the measurement of the thermal conductivity value of a drilled sample is avoided, and the research expenditure in research work such as geodetic heat flow is reduced; according to the method, the thermal conductivity value of the sandstone is calculated by utilizing the volume content percentage of minerals in the sandstone, so that the phenomenon that weightpercentage data measured by an XRD (X-Ray Diffraction) experiment does not accord with the mineral content of a traditional thermal conductivity prediction model is avoided; according to the method,the rock thermal conductivity data is calculated by utilizing the density of the sample minerals, and experimental data is more accurate than original rock thermal conductivity data calculated by utilizing the general density value of the minerals and conforms to actual conditions.
Owner:CHINA UNIV OF MINING & TECH

Method for measuring multiple parameters of retina of living mouse

The invention discloses a method for measuring multiple parameters of retina of a living mouse. The method comprises the following steps: fixing a mouse, which is successfully anesthetized, on a three-dimensional experiment table; assembling a plano-concave lens, a front lens and an optical coherence tomography (OCT) instrument; adjusting the three-dimensional experiment table to ensure that the eyeballs of the mouse are positioned right ahead the front lens, and that a clear retina scanning eye fundus image appears on a display screen of the OCT instrument; selecting a high-definition one-line scanning mode to obtain a cross-sectional image of the total retina of the mouse, and measuring the thickness of each layer on the retina of the mouse; selecting a retinal volume mode, aligning the center of a cross scanning line with the optic disk of the mouse to measure the average thickness and volume of nine subareas of the retina of the mouse, and reconstructing the form of the retina of the mouse in a three-dimensional mode; and finally, selecting an optic disk scanning mode to acquire continuous thickness data of a nerve fiber layer of the retina. The method has the characteristics of high simplicity and feasibility, high repeatability, accurate result and zero external environment interference and is suitable for popularization and application.
Owner:EYECURE THERAPEUTICS INC JIANGSU

Multifunctional gas wave refrigerating jet stream field displaying apparatus and method for measuring

A kind of multiple functions gas wave cooling effluxion flow filed display device and measuring method which belongs to technique field of effluxion of pressure gas and expanded cooling and measuring control. The invention adopts integrated structure and works when running and static and possesses window for view and shooting image, it can analogue working mechanism and performance of multiple types of gas, and provides platform for leading high-precision PIV to wave mechanical flow filed measurement, and measures important parameters in-phase, and fulfills requirement of experiment, and accomplishes research content of changing condition, experiment component, rotate speed, two phases of gas and liquid, and accomplishes important experiment of best matching of structure and parameter. The structure is reasonable and easy, operation is convenient, and performance is stable and reliable. It fits for multiple functions gas wave cooling measuring filed of changing condition and research content.
Owner:DALIAN UNIV OF TECH

Fastening piece for compressed column test of building structure

The invention provides a fastening piece for a compressed column test of a building structure, which relates to a protective device for axially compressing or bias compressing a specimen column end in a building structure test. The protective device comprises a steel plate and bolts, wherein a lug which is folded outward is arranged at the end part of the steel plate; a center drilling hole without screw threads is arranged at the center of the lug; a small hole with screw threads is arranged at the center of the steel plate; the center drilling hole is matched with a connecting bolt for use;and the small hole is matched with a fastening bolt for use. The protective device can solve the problems existing in the current building structure test to ensure that the loaded column end can not be damaged before a specimen reaches the ultimate bearing capacity. In the test of a concrete filled steel tubular (CFST) column, the protective device of the invention can also be used for avoiding the defect that the welding quality between a tube wall at the column end and a loaded plate is poor so that the column end is bent firstly.
Owner:中国建筑东北设计研究院有限公司

Plant transgenic visual tracking expression system and application thereof

The invention discloses a plant transgenic visual tracking expression system and application thereof. The plant transgenic visual tracking expression system is a plant expression vector which contains a visual marker gene expression kit, the visual marker gene expression kit consists of an expression kit of a transcription factor bi gene in the anthocyanin biosynthetic pathway and an expression kit of a transcription factor cl gene in the anthocyanin biosynthetic pathway. The color regulator gene is applied on plant genetic transformation operation, the screening work is effectively relieved after the transformation, according to the gene performance on the plant, the valuable transformation materials can be selectively kept, accordingly, the screening time and the research cost are greatly saved, the accuracy and the reliability of the experimental result are greatly improved, so good foundation is laid for carrying out analysis work after the transformation. In addition, the transient expression of the color gene is utilized, the transformation effect can be visually and more directly analyzed, and accordingly, various transformation influencing factors are prevented from being generated.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Method for preparing soil for analogue test

The invention discloses a manufacture method for analysis earth that includes the following steps: taking physical characteristic analysis to parent soil and the matter properties experiment of particle, proportion, density, unit weight and permeability coefficient; gaining the particle class curve, density, penetrating parameter from the experiments; fetching sample earth to make it close to the particle class curve; taking mechanical property and analysis of triaxiality compression shear; when the sample earth has the corresponding stress-strain, deformation-strain and penetrating parameter, taking large quantity matching according to the sample earth. The invention has close statics feature as undisturbed sand that could sharply save research outlay and shorten research process.
Owner:INST OF MECHANICS CHINESE ACAD OF SCI

Method and device for collecting multi-layer soil water sample

The invention discloses a method and a device for collecting multi-layer soil water samples. The method comprises the following steps of: conveying the water samples collected by a sampling tube in the soil of different depths to a corresponding water dividing bin in a water dividing tank by a first pumping device; and conveying the water samples in the water dividing bin to a corresponding watercollecting tube by a second water pumping device. The sampling tube is sealed, and a number of clay heads are arranged on the tube wall for infiltrating the soil water. The pumping device is controlled by a microcomputer, so that the various components work together to effectively collect the soil water samples of different soil depths. The efficiency of collecting groundwater samples is improved,and batch, differential, and simultaneous water sample collection can significantly improve the accuracy of batch sampling time intervals and improve the accuracy of test data. The automatic, regularor preset time water sample collection can be realized by the microcomputer, which greatly reduces the difficulty of manual collection, and the situations that water samples cannot be collected manually and the collected water samples are mixed due to natural weather and other factors can be avoided, which bring great significance to the progress of isotope hydrology.
Owner:HOHAI UNIV

Non-uniform pressure field test device and test method in viscous medium pressure forming

ActiveCN104588440BEasy to study changing stateEasy to useMetal working apparatusWrinkle skinInstability
The invention discloses a device and a method for testing a non-uniform pressure field of a viscous medium during pressure forming, and relates to a test device and a test method. The invention aims to solve the problems that the existing method and device cannot easily test deformation behaviors of instability wrinkling and wrinkle elimination under the action of various non-uniform velocity fields and pressure fields during necking of a large-diameter-ratio thin-wall or ultrathin-wall pipe, and each mold device can only test the influence of one loading mode on the non-uniform pressure field on the surface of a cylinder blank each time. The device disclosed by the invention is reasonable in structural design and is comprehensive and accurate in test data. The test method comprises the following steps: injecting the viscous medium by multiple pistons at different speeds; measuring the injection pressure in real time by virtue of a pressure sensor; and controlling the injection pressure of the viscous medium by virtue of reciprocating of the pistons to form multiple non-uniform pressure fields and velocity fields in a medium cabin so as to ensure that a pipe blank is subjected to necking deformation under different conditions. The device and the method disclosed by the invention are used for researching the influence of a loading mode of the viscous medium on pressure diameter reduction of a pipe blank viscous medium.
Owner:HARBIN INST OF TECH

Method for characterizing epoxy resin composite material interface mechanism

A method for characterizing the interface mechanism of epoxy resin composite material relates to a method for characterizing the interface mechanism of epoxy resin composite material, which solves the problems, such as high requirement for technology, high cost in experiment constructed by Au element and instable effect of experiment, of the research of interface phenomena of epoxy resin composite material through the current molecular self-assembly film technology. The method comprises: 1, simulation to obtain self-assembly modeling system; 2, establishment of the interface structure of the modeling system and epoxy resin; 3, calculation for the interface structure through molecular dynamics to evaluate the effects of different functional groups on the interface performance of epoxy resin composite material. The invention is easy to implement without actual operation, requirement in operating skill or use of Au element to save a lot of research fees with stable and reliable experimental result and greatly reduced experimental time and period.
Owner:HARBIN INST OF TECH

Rapid preparation method for lumen grinding disc of deep-sea foraminifera

The invention relates to the disciplines of marine biology, paleoceanography and sedimentary geology, can be applied to the field of the identification research of the species of foraminifera, the research of marine environment indication, the research of paleoenvironmental reconstruction and global change, and the like, and in particular provides a rapid preparation method for a lumen structure grinding disc of deep-sea foraminifera. The foraminifera obtained from the separation of deep-sea sediments are subjected to gum dipping lumen, hanging drop embedding, positioning fixation and water-grinding disc processing, that is, a complete grinding disc of the lumen structure of the deep-sea foraminifera is quickly obtained. The method provided by the invention, through the layer information obtained by the grinding disc, can more accurately identify the species of foraminifera, avoids scientific confusion such as identification errors caused by external morphological variations such as the shape, texture and number of compartments of the worm body, and provides accurate scientific basis and quick problem solving solutions for the disciplines of accurate identification of the species of deep-sea foraminifera, the research thereof of marine environment indication, the research of paleoenvironmental reconstruction, the research of global change, and the like.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI +1

Rapid Preparation Method of Internal Cavity Polishing of Deep-sea Foraminifera

The present invention relates to marine biology, paleoceanography, sedimentary geology and other disciplines, and can be applied in the fields of foraminifer species identification research, marine environment indication research, paleoenvironment reconstruction and global change research, etc. Specifically, it is a A rapid preparation method for deep-sea foraminifera lumen structure grinding plate. Foraminifera isolated from deep-sea sediments were treated with gum immersion, hanging drop embedding, position adjustment and water grinding, that is, complete grinding of the inner cavity structure of deep-sea foraminifera was quickly obtained. The invention can more accurately identify the species of the foraminifera through the level information obtained by grinding, and avoid scientific confusion such as identification errors caused by external morphological variations such as the shape, texture, and number of chambers of the foraminifera. The invention provides accurate scientific basis and quick problem-solving scheme for the accurate identification of types of deep-sea foraminifera and the research on indications to the marine environment, paleoenvironment reconstruction research, global change research and other subject fields.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI +1

Fan blade fatigue limit attenuation intelligent monitoring method based on image recognition

PendingCN112288135ARapid prediction of fatigue limitFast Prediction of Fatigue Limit AttenuationGeometric CADImage enhancementPrediction algorithmsStructural engineering
The invention discloses a fan blade fatigue limit attenuation intelligent monitoring method based on image recognition. The method comprises the steps that S1, building a fan blade rapid microscopic analysis system; S2, shooting the surfaces of a new fan blade and an old fan blade according to a sweeping mode to obtain complete micrographs of different blades; S3, analyzing a micrograph, and extracting damage characteristics of scratches, cracks and pits on surfaces of leaves; S4, quantifying state parameters of blade surface damage ; S5, carrying out vibration fatigue tests on the new fan blades and the old blades with different service duration; S6, obtaining fatigue limit data of the blades; S7, realizing a method for establishing a response surface through training data based on an artificial intelligence algorithm of a small sample; and S8, establishing a fatigue limit recession intelligent prediction algorithm based on the blade surface state characteristics to obtain a corresponding relationship between the blade surface damage state parameters and the blade fatigue limit degradation degree. The invention can quickly predict the attenuation of the fatigue limit of the blades.
Owner:NANJING UNIV OF AERONAUTICS & ASTRONAUTICS

A bridge head settlement compensation amount testing device and testing method

The invention discloses a bridgehead settlement compensation amount test device and method. The device comprises a pressurization unit, a model case and a control system. The pressurization unit comprises a frame, an oil cylinder, a pressure head and a guiding mechanism. The frame has a U-shaped structure and comprises a first support leg, a second support leg and a crossbeam. The oil cylinder is arranged in the middle of the crossbeam, the cylinder body of the oil cylinder is fixedly connected to the crossbeam, the end of a piston rod is provided with the pressure head and the guiding mechanism is arranged between the pressure head and the frame. The model case comprises a case body, a roadbed pavement model, a displacement sensor and several steel pipes. The roadbed pavement model is arranged in the case body. The upper part of the case body is provided with the displacement sensor. The several steel pipes go through the case body and are connected to the case body. The steel pipes are provided with water leakage apertures. Through simulation of different pavement structures, a relationship of a swelling amount increasing rule, compensation layer position, thickness, lateral confinement and compaction rate, and a lime slurry injection amount is researched and the relationship lays the foundation of design index formation.
Owner:SHANDONG JIAOTONG UNIV

Method and apparatus for fouling monitoring and prediction in combustors

The invention provides a method and device used for monitoring and predicting scales in a combustor. The method comprises steps of by acquiring one kind of or various kinds of combustion signals of the combustor and / or a gas system corresponding to the combustor; determining combustor performance models corresponding to the combustion state signals according to the combustion signals; acquiring acombustor performance reference model corresponding to the combustor; and finally, according to the combustor performance model, by combining the combustor performance reference model, determining early warning information corresponding to the combustor. Thus, the generated combustor performance model is quite practical; by use of the method and the device, large quantity of research funds and usecost can be reduced; the scales and the development trend of the combustor can be monitored and predicted in a quite simple and convenient mode; and the method and the device can be used for strategyplanning of maintenance of economical combustors or gas turbines.
Owner:SIEMENS ENERGY GLOBAL GMBH & CO KG

Method for calculating mudstone thermal conductivity by using mineral content

ActiveCN111948247AAvoid the phenomenon of non-compliance of mineral contentAvoid errorsMaterial analysis using wave/particle radiationMaterial thermal conductivityHeat flowMaceral
According to the method for calculating the mudstone heat conductivity by utilizing the mineral content, the existing mineral data is utilized to predict and calculate the heat conductivity value of mudstone, so that the measurement of the heat conductivity value of a drilling sample is avoided, and the research expenditure in research work such as geodetic heat flow is reduced; the volume contentpercentage of minerals in mudstone is used for calculating the heat conductivity value of mudstone, and the phenomenon that weight percentage data measured through an XRD experiment does not conformto the mineral content of a traditional heat conductivity prediction model is avoided. The mineral thermal conductivity entropy of the mudstone is used for calculating the thermal conductivity of themudstone, and errors caused by a mineral space structure in the mudstone due to a traditional calculation method are avoided.
Owner:CHINA UNIV OF MINING & TECH

Method and system for determining the structure of long-chain organic compounds using carbon spectrum data

The invention provides a method and a system for determining a long chain organic compound structure by using nuclear magnetic resonance carbon spectrum data. The method comprises the following steps of inputting the carbon spectrum data of the long chain organic compound with an undetermined structure, and setting a parameter condition of the input carbon spectrum data, wherein the parameter condition comprises a deuterium reagent and a tolerance value; extracting the carbon spectrum data of an organic compound with a known structure matched with the deuterium reagent in the parameter condition from a database, and reading the carbon spectrum data into a temporary table; circularly comparing each group of carbon spectrum data in the temporary table and the input carbon spectrum data, wherein each group of carbon spectrum data in the temporary table comprises precise carbon spectrum data and non-precise carbon spectrum data; recording the number of carbon spectrum values of each group of carbon spectrum data in the temporary table successfully matched with the input carbon spectrum data; calculating the matching similarity of each group of carbon spectrum data in the temporary table and the input carbon spectrum data. By the method, research time is reduced, the scientific research efficiency is improved, and the scientific research cost is reduced.
Owner:SHANGHAI MICRONMR INFOR TECH CO LTD

Recombinant Salmonella expressing red fluorescent protein and its construction method

ActiveCN105695384BReduce false positivesSolve real-time monitoring problemsBacteriaMicroorganism based processesImmunofluorescent stainCo localization
The invention relates to recombinant salmonella capable of expressing red fluorescence protein and a building method of the recombinant salmonella. The clinically isolated salmonella is utilized as a transformation receptor, plasmids pFPV-mCherry with red fluorescence protein gene segments inserted enter a bacterium through electroporation, and the salmonella capable of expressing the red fluorescence protein is built accordingly. The built recombinant salmonella can be used for marking and tracing in-vitro and in-vivo test processes of the salmonella, and the problem that the real-time monitoring cannot be performed in the salmonella test is solved; the recombinant salmonella can express the fluorescence protein and can replace an expensive salmonella specific antibody, not only is the research grant saved, but also the false positive caused by utilization of the antibody is greatly reduced; co-localization can be performed through combination with immunofluorescent staining with related protein, and an effective means is provided for in-depth study of the clinically isolated salmonella.
Owner:CHINA AGRI UNIV

Time and temperature control equipment for tissue specimen fluorescence staining technology

The invention provides time and temperature control equipment for fluorescent staining of tissue specimens, which solves the problem of too many uncertain factors affecting experimental results in artificial operation steps in fluorescent staining experiments. The invention comprises a box body, on which a vertical pole is arranged, on which a dripping mechanism capable of controlling the infusion amount of the fluorescent agent is arranged, and a test chamber for constant temperature heating and preservation of samples is arranged on the box body below the dripping mechanism. The sample constant temperature storage mechanism, the sample constant temperature storage mechanism can be rotatably installed on the box body, and the box body on the right side of the sample constant temperature storage mechanism is provided with a movable installation platform, which is used to install the fluorescence microscope; the box body is also equipped with There are consoles for controlling the instillation mechanism and the constant temperature storage mechanism for samples. The present invention uses electrical control means to replace the original manual operation steps, which greatly improves the accuracy of the experimental results, effectively avoids the abandonment of experiments due to human accidents, and can save a lot of research time and research funds.
Owner:HENAN UNIV OF CHINESE MEDICINE

A method for measuring multi-parameters of living mouse retina

The invention discloses a method for measuring multiple parameters of retina of a living mouse. The method comprises the following steps: fixing a mouse, which is successfully anesthetized, on a three-dimensional experiment table; assembling a plano-concave lens, a front lens and an optical coherence tomography (OCT) instrument; adjusting the three-dimensional experiment table to ensure that the eyeballs of the mouse are positioned right ahead the front lens, and that a clear retina scanning eye fundus image appears on a display screen of the OCT instrument; selecting a high-definition one-line scanning mode to obtain a cross-sectional image of the total retina of the mouse, and measuring the thickness of each layer on the retina of the mouse; selecting a retinal volume mode, aligning the center of a cross scanning line with the optic disk of the mouse to measure the average thickness and volume of nine subareas of the retina of the mouse, and reconstructing the form of the retina of the mouse in a three-dimensional mode; and finally, selecting an optic disk scanning mode to acquire continuous thickness data of a nerve fiber layer of the retina. The method has the characteristics of high simplicity and feasibility, high repeatability, accurate result and zero external environment interference and is suitable for popularization and application.
Owner:EYECURE THERAPEUTICS INC JIANGSU
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