73 results about "Co localization" patented technology

The invention provides a new assay format for high throughput molecular binding studies at a single molecule level. The invention enables creation of binding event identifiers in a highly parallel way. Individual binding events occur between two agents of a binding pair, e.g., a protein-based binding pair or a binding pair comprising a protein and a chemical moiety. The binding event identifier created through the binding of the two binding agents is unique to that pair, and identification of the binding event identifier is indicative of the binding of these specific may be assessed through a readout that is digital in nature. The invention enables very large sets of thousands or more of different binding agents or potential binding agents to be assayed simultaneously, resolving millions or more of potential interactions, and distinguishing specific interactions from those that are less specific.

The invention relates to methods of detecting nucleic acids, including methods of detecting one or more target nucleic acid sequences in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support or suspending cells are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. The invention further relates to methods to improve probe hybridization specificity and their application in genotyping. The invention also relates to in situ detection of mis-joined nucleic acid sequences. The invention relates to reducing false positive signals and improve signal-to-background ratio in hybridization-based nucleic acid detection assay. The invention further relates to method to improve specificity in hybridization based nucleic acid using co-location probes. Compositions, tissue slides, sample of suspended cells, kits, and systems related to the methods are also described.

The invention relates to a fluorescent dye based on naphthalimide, and a preparation method and an application thereof. Specifically, the invention provides a naphthalimide fluorophore which has a structure as shown in a general formula I which is described in the speicification, wherein groups in the formula I are described in the specification. The compound provided by the invention has good water solubility and high fluorescent quantum yield in an aqueous solution, and can be used for two-photon imaging and co-localization imaging to living cells.

The invention discloses a multi-robot co-localization and fusion mapping method under multi-view in an open space. The method comprises the following steps of: completely covering a detection scene byusing multiple observations in the air and the ground, integrating scene image data collected by aerial robots and ground robots, and positioning the robots by visual constraints and restoring three-dimensional scene information; optimizing three-dimensional point cloud map of the mapping and positioning system and six-degree-of-freedom pose of the robots by specific visual features adhered to the robot. The pose optimization and map fusion algorithm based on visual features significantly improve reconstruction and positioning accuracy, revise map scales, so that local maps of each robot canbe shared among multiple heterogeneous robots, the coverage of three-dimensional reconstruction is improved, the reliable environmental information is quickly provided for mission planning, disaster environment search and rescue, and military anti-terrorism.

The invention relates to methods of detecting nucleic acids, including methods of detecting one or more target nucleic acid sequences in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support or suspending cells are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. The invention further relates to methods to improve probe hybridization specificity and their application in genotyping. The invention also relates to in situ detection of mis-joined nucleic acid sequences. The invention relates to reducing false positive signals and improve signal-to-background ratio in hybridization-based nucleic acid detection assay. The invention further relates to method to improve specificity in hybridization based nucleic acid using co-location probes. Compositions, tissue slides, sample of suspended cells, kits, and systems related to the methods are also described.

The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.

The invention relates to the field of biotechnology, and in particular to a method for effectively anchoring a candidate gene region of a quantitative trait. The method is co-localized with a genome-wide association study and a quantitative trait localization method to achieve efficient anchoring of the quantitative trait candidate gene regions. The method achieves effective anchoring of the candidate gene region of the peanut quantitative trait by co-localization with the genome-wide association study and the quantitative trait localization. The method is exemplified by quantitative granule weight, firstly, through a simplified genome sequencing technology, the genome-wide SNP marker development of 165 cultivar peanut core collections in China is carried out; based on a principle of linkage disequilibrium, combined with peanut 100-fruit weight and 100-kernel weight breeding value, the related gene regions related to peanut weight traits are associated; in the previous reports, the QTLmapping results of the grain weight traits are converted to the corresponding genomic physical locations, the co-localization region of the two methods can be obtained, and the method can effectivelyand accurately realize the localization of the genome-wide particle weight-related trait genes.

Methods for controlling surface topography and binding reactivity in functionalized lipid layers, including in the form of liposomes, using pH-dependent processes. During direct cell-to-cell communication, lipids on the extracellular side of plasma membranes reorganize, and membrane associated communication-related molecules co-localize. At co-localization sites, sometimes identified as rafts, the local cell surface topography and reactivity are altered. Integration of these processes on nanometer-sized lipid vesicles used as drug delivery carriers would precisely control their interactions with diseased cells minimizing toxicities. Included are pH-dependent processes on functionalized lipid bilayers demonstrating reversible sharp changes in binding reactivity within a narrow pH window. Cholesterol enables tuning of the membrane reorganization to occur at pH values not necessarily close to the reported pKa's of the constituent titratable lipids. One illustrative function of the invention is to use liposomes to deliver bioactive agents to cancer or tumor cells and compositions of specific lipids that form liposomes to deliver a biologically active agent.

The invention relates to an oleaginous yeast lipid droplet protein, coding gene and application thereof. The oleaginous yeast lipid droplet protein is the protein shown as the following (a) or (b): (a) a protein composed of an amino acid sequence shown by SEQ ID NO:1; and (b) a SEQ ID NO:1 derived protein that is obtained by subjecting the amino acid of SEQ ID NO:1 to substitution and / or deletion and / or adding by one or several amino acids and has related activity of the lipid droplet protein. Green fluorescent protein co-localization and grease fermentation analysis show that the oleaginous yeast lipid droplet protein and its coding gene provided by the invention can significantly improve the cellular grease accumulation and storage capacity. According to the invention, a recombinant engineering strain able to significantly improve grease accumulation can be obtained by heterologous expression of gene. The oleaginous yeast lipid droplet protein and its coding gene provided by the invention can be applied to construction of strains producing microbial oil and fatty acid derivatives, and can be used as the target for screening of drugs treating obese patients.

The invention discloses the application of a fluorescent probe in the detection of hydrogen peroxide molecules. The fluorescent probe is used for sensing and detecting the content of hydrogen peroxide in the water environment and cell lysosomes; the sensing detection includes Fluorescence detection, visual qualitative detection, cell imaging detection. The invention realizes the selective and rapid detection of the H2O2 molecular probe, and has good selectivity and strong anti-interference ability of other molecules. In addition, the change of the color of the solution can be observed with the naked eye, and the change of the fluorescent color can also be observed under the ultraviolet light. It is a fluorescent probe with a color sensing function. This probe can be applied to the detection of hydrogen peroxide in intracellular lysosomes. Compared with commercial lysosomal dyes, it has a high overlap rate for imaging hydrogen peroxide in lysosomes, and the co-localization of the two dyes The coefficient is 0.94.