The invention relates to a built-in steel column fixing system and an application construction method thereof. The built-in steel column fixing system includes a column base foundation, a connecting rod, an embedded steel plate and a column base plate. The column base foundation is provided with a foundation pit, and a fixing net is laid in the foundation pit; one end of the connecting rod is connected with the fixing net, and the other end of the connecting rod penetrates through the embedded steel plate and is connected with the embedded steel plate; the column base plate is arranged to be in parallel with the embedded steel plate, the column base plate is provided with a connecting hole, and the connecting rod passes through the connecting hole and is connected with the column base plate after penetrating out of the embedded steel plate; and a slurry layer fills a space between the embedded steel plate and the column base plate. According to the built-in steel column fixing system,by adopting the fixing system, the accuracy of steel column in-position is improved; and the slurry layer directly fills the space between the embedded steel plate and the column base plate, so that the compactness of the embedded steel plate and the column base plate is strengthened, the possibility of steel column deviation is reduced, and the horizontal accuracy is improved. In addition, the application construction method of the built-in steel column fixing system is simple in steps and convenient to operate, thus being suitable for popularization.