The invention discloses an application of a three-dimensional
fluorescence spectrometry in identification of
paralytic shellfish toxin-producing microalgae. The method comprises the following steps: extracting three-dimensional
fluorescence spectrum information of PSP-producing
algae and PSP-non-producing
algae growing under different environmental conditions, extracting characteristic peaks of experimental
algae by using a Coif2
wavelet function, performing clustering analysis by using a
system clustering method, removing abnormal spectrums, and screening standard spectrums to obtain a Coif2
fluorescence characteristic standard spectrum
library; when the discriminating function established according to the fluorescence characteristic standard spectrum
library is used for discriminating the PSP-producing algae and the PSP-non-producing algae, the discriminating correct rates are respectively 77.3% and 84.5%, the correct rates are very high, and the purpose of quickly and accurately identifying the
toxin-producing algae is basically achieved. The invention provides a
research result of the application of the three-dimensional
fluorescence spectrometry in identification of paralyticshellfish
toxin-producing microalgae for the first time, and the three-dimensional
fluorescence spectrometry can be applied to the aspects of
red tide algae identification sensors or portable algae fluorescence identification instruments and the like.