Fluorescence characteristic standard spectrum library for identifying paralytic shellfish poisoning-producing microalgae as well as construction method and application of fluorescence characteristic standard spectrum library
A technology of shellfish toxins and construction methods, which is applied in fluorescence/phosphorescence, character and pattern recognition, instruments, etc., can solve the problems of poor timeliness, restrictions on on-site management of shellfish toxins, poor repeatability, etc.
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[0062] 1. Experimental method
[0063] 1. Cultivation of algae:
[0064] The algae were cultured with natural seawater (pH 7.5±0.1, salinity 27‰, purchased from Guangzhou Huangsha Aquatic Products Market), filtered with a 0.45μm microporous membrane, and then sterilized at 121°C and 15psi for 25min , cooled to room temperature. The algal culture solution was prepared with the f / 2 improved formula, placed in an artificial climate box after inoculation, the temperature was 25 °C, and the light intensity was 150 μmolm -2 s -1 , cultured under the condition of light-dark cycle L:D=12:12. Three parallel samples were set up for each algae, and samples were taken at a fixed time every other day. After the algae was fixed, the algal cells were counted under the microscope and the growth curve was drawn.
[0065] 2. Fluorescence spectrum determination of algae in different environments:
[0066] Set the light intensity to 60 μmolm -2 s -1 , 120μmolm -2 s -1 , 200μmolm -2 s -...
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