55results about How to "Not limited by season" patented technology

The invention provides a suspension-cultured cell of Acer ginnala Maxim for producing gallic acid, which is characterized in that a proper amount of callus of Acer ginnala Maxim is inoculated in a solid cultivation medium containing WPM+TDZ 0.002-0.01 mg / L+6-BA 0.05-0.15 mg / L for successive transfer culture for 15 to 20 days to obtain a first successive generation; a well-growing callus is transformed into a triple WPM liquid medium containing a large amount of elements and having a pH value of 5.8 to 6.0, in the presence of a hormone including TDZ 0.002 to 0.010 mg / L and 6-BA 0.05 to 0.15 mg / L and 2% to 3% sugar to obtain the fresh weight of an inoculum size of 20 to 40 g / L; and the culture conditions comprises the temperature of 23 to 25 DEG C, the rotation speed of 110 to 130 rpm, the illumination time of 8 to 10 h / d, the illumination intensity of 1,400 to 1,700 Lx, and the culture period of the first successive generation of 7 to 15 days. The cells are collected after one period of 20 to 30 days, with the fresh weight up to 478.2 g / L and the dry weight up to 22.3 g / L, wherein the content of gallic acid is up to 2.29%, the output of gallic acid is 0.51g / L, and the gallic acid with the amount of 6.1227 g / L can be collected in one year. The invention can improve the output of suspension of gallic acid in Acer ginnala Maxim by cell engineering method for the first time, which solves the raw material problem for the production of gallic acid. The invention has the advantages of easy operation, short cycle, low cost as well as greatly saved labor force and material resource,improved labor productivity and protection of environment and Acer ginnala Maxim resources.

The invention relates to a sand-protecting barrier prepared from biodegradable materials, comprising a biodegradable polylactic acid fiber (PLA) textile, wherein the polylactic acid fiber (PLA) textile is made to a drum-shaped flush system sand-protecting barrier and a drum-shaped low vertical type sand-protecting barrier, diameters of the flush system sand-protecting barrier and the low verticaltype sand-protecting barrier are 8-25 cm, and the height of the drum-shaped low vertical type sand-protecting barrier is 15-35 cm. A preparation method of the sand-protecting barrier comprises a flush system preparation method and a low vertical type preparation method, wherein the flush system preparation method and the low vertical type preparation method both comprise a grid type preparation method and a row type preparation method. The flush system sand-protecting barrier and the low vertical type sand-protecting barrier which are prepared by polylactic acid fibers (PLA) overcome secondary pollution and have low cost. The grid type preparation method and the row type preparation method adopted by the flush system sand-protecting barrier and the low vertical type sand-protecting barrier ensure the effective protection of the sandy soil of protected areas, prevent the sandy soil loss, are simple and have obvious effect.

The invention relates to a bud stock grafting method of Australian nuts. The grafting method comprises the following steps of: using Australian nuts for dibbled planting and culturing seedling sproutsof the nuts as bud stocks, using one-year-old branches which are lignified on the seed tree of Australian nuts as scion and grafting the branches on the bud stocks, temporarily planting the bud stocks in nutritional bags, planting the obtained nutritional bags with bud stocks on a seedling bed with shade net sheds and mulching film convex sheds and controlling the temperature and relative humidity in the sheds. The bud stock grafting method fully exerts the characteristics of strong regeneration capacity and vigorous growth of young tissues of the bud stocks, and effectively overcomes the defects that the forming layers of the young seedling stocks of the nuts are thin and the growth and coalescence are harder with the scions and grafting survival rate is low, thus greatly improving the grafting survival rate of the Australian nuts by more than 20 percent, shortening the period of out of nursery by more than one year, reducing the culturing cost of seedlings and increasing the economic benefit.

The invention belongs to the field of biotechnology and discloses a method for identifying soybean cyst nematode-resistant No. 4 race soybean resources and special SSR primers. The SSR markers identified against race 4 of soybean cyst nematode provided by the present invention consist of Satt533 and Satt504. The invention is verified through experiments on resistant and susceptible soybean resources from home and abroad, and the identification method of the invention is simple to operate, economical and efficient, and the result is stable and reliable. Compared with the diseased soil identification method, it has the advantages of saving time and effort, and is not limited by seasons. It has a better application prospect and better serves the identification and evaluation of soybean cyst nematode resistant race 4 in production practice. Therefore, the combination of SSR markers and the identification method for soybean cyst nematode-resistant race 4 of the present invention will have broad application prospects in the screening of soybean cyst nematode-resistant race 4 resources and variety breeding.