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Ficus carica living body bleaching sap minimal invasive collecting device

A collection device and wound fluid technology, which is applied in the field of minimally invasive collection devices for live fig wound fluid, can solve the problems of lengthy production links and difficulty in effectively ensuring protease activity, and achieve the effect of easy disassembly

Inactive Publication Date: 2019-02-15
江苏龙山湖生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lengthy production process, it is difficult to effectively guarantee the activity of protease in the final product
It has been reported in the literature that in conventional crude ficin enzyme products, the enzyme activity is only 17-20% of that of ficin in the fresh sap of figs
In addition, the acquisition of fig fruits, leaves, and stems is limited by season and growth stage, and only seasonal production can be carried out

Method used

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  • Ficus carica living body bleaching sap minimal invasive collecting device

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Effect test

Embodiment 1

[0021] Such as figure 1 As shown, the present embodiment provides a minimally invasive collection device for wounded liquid of living figs, consisting of a liquid suction needle 1, a negative pressure liquid collection device 2 and an air suction device 3 connected in sequence from left to right, the liquid suction needle 1, The hose 4 is used to connect the negative pressure liquid collection device 2 and the suction device 3 .

[0022] The liquid-absorbing needle 1 is specifically made of a stainless steel tube with a model number of 304, an inner diameter of 1.6-2 mm, and a length of 40 mm. It is cut with a cutting machine, and the angle between the plane of the cutting piece and the longitudinal direction of the stainless steel tube is 45°. After the oblique cut is completed, a sharp hollow needle is formed, which can be inserted relatively easily into lignified shoots, petioles and fruits.

[0023] The negative pressure liquid collection device 2 includes a negative pres...

Embodiment 2

[0029] The difference between this embodiment and Embodiment 1 is that: the liquid collection bottle 22 is also pre-filled with a wound fluid protection solution, and the wound fluid protection liquid is an aqueous solution of tartaric acid with a pH of 5.5-6.0. The concentration of tartaric acid aqueous solution is 0.1-0.15M.

[0030] Specifically, the tartaric acid aqueous solution used in this embodiment has a concentration of 0.1 M and a pH of 5.5.

[0031] After testing, the collection object was petiole, the obtained enzyme activity was 14.12 U / g.FW, the total protein content was 3.81 mg / g.FW, and the specific activity was 3.87 U / mg.pro. The collection object is the fruit base, and the obtained enzyme activity is 12.56 U / g.FW, the total protein content is 4.51 mg / g.FW, and the specific activity is 3.78 U / mg.pro.

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Abstract

The invention discloses a ficus carica living body bleaching sap minimal invasive collecting device. The device comprises a liquid suction needle head, a negative pressure liquid collecting device andan air suction device which are sequentially connected, the liquid suction needle head, the negative pressure liquid collecting device and the air suction device are connected through hoses; the negative pressure liquid collecting device comprises a negative pressure liquid collecting cavity and a liquid collecting bottle, the part between the negative pressure liquid collecting cavity and the liquid collecting bottle is through, and the liquid collecting liquid is detachable. According to the collecting device, the swelling of ficus carica fruit root systems is utilized to directly performing minimal invasive operation on a ficus carica plant to collect the bleaching sap which contains higher concentration of ficus carica protease, and the activity of the ficus carica protease is preserved to the maximum extent.

Description

technical field [0001] The invention belongs to the technical field of plant collection devices, and in particular relates to a minimally invasive collection device for wound fluid from living figs. Background technique [0002] Ficin is a kind of thiol protease, which mainly exists in the latex and receptacle protein of figs. It is a plant protease with a wide range of uses. In addition to participating in the decomposition and migration of proteins, it is also related to the conduction of cell signals. The protease extracted and purified from figs is widely used in the fields of food processing, industrial production and medical health because of its good stability, strong proteolytic ability, and good degradation effect on various proteins. It largely replaces papain and is used in various industries, and has broad industrial development potential. [0003] At present, the main extraction method of fig protease is to collect isolated fig fruit, leaves or stem segments, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G23/14A01G23/10
CPCA01G23/10A01G23/14
Inventor 王成郭强吴俊峰解伟戴龙艳郭仲歆
Owner 江苏龙山湖生物科技有限公司
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