The invention discloses a method for efficiently and rapidly regenerating adventitious buds from
pear leaves. The method comprises steps as follows: acquiring sterile
tissue culture seedlings, generating adventitious buds by inducing the leaves and performing
primary culture and subculture on the adventitious buds, wherein an inducing medium is a combination of a basic culture medium NN69,
auxin IBA and
cytokinin TDZ, the leaf age is about 20-50 d, dark culture is performed for 21 d, the regeneration effect of leaves of
Pyrus bretschneideri Rehd. is remarkable, the regeneration rate can reach 70.83%, the average number of regeneration buds of each leaf is 2.06, the
callus occurrence rate reaches 100%, the
browning rate is very low and is even 0, and demands for
germplasm preservation of
pear varieties and requirements for the regeneration rate of the
Pyrus bretschneideri Rehd. serving as a genetic transformation material are met. Requirements for the leaves are low, the material sources are enriched, and the problem of high
browning rate in the
woody plant tissue regeneration process is solved. The problem that the regeneration rate of the oriental
pear varieties is generally low is effectively solved, an effective material is expected to be provided for genetic transformation of the oriental pear varieties, and breakthrough of genetic transformation of pears is realized.