36results about How to "No loss of time" patented technology

An automatic keystone correction which enables even an inexperienced user to easily obtain an image corrected using the keystone distortion during a tilted projection. The projector detects varying of its elevation angle by using an elevation detecting module. When the angle stops varying, the projector determines that the elevation adjustment by the user ends, and executes auto keystone correction of the input image according to the elevation angle.

The invention discloses riemerella anatipestifer detection kit and method based on a loop-mediated isothermal amplification technology. Based on six specific regions of a 16S rRNA conserved region of riemerella anatipestifer, two specific primers and two specific outer primers are designed in the kit and can specifically distinguish the riemerella anatipestifer and other serotype salmonellae so as to ensure the high specificity and the reliability of a detection result of loop-mediated isothermal amplification. The invention detects the riemerella anatipestifer on the basis of the loop-mediated isothermal amplification technology, can amplify a target sequence rapidly, efficiently and specifically under the isothermal condition, has simple and convenient operation and does not use expensive instruments and reagents; an amplification product can be developed directly by using a fluorescent dye and a result can be judged with naked eyes; the detection cost is low; and the invention is particularly suitable for small and medium size units and field tests.

The invention relates to a cleaning device for gluing nozzles (1) arranged to apply glue (32) to tobacco processing material (33) or packaging material, wherein a device for at least one gluing nozzle (1 ) means (2, 18) for supplying a liquid (3, 14), the liquid supply means comprising at least one liquid outlet (2). The invention also relates to a method for cleaning a gluing nozzle (1) of a tobacco processing machine or a packaging machine, wherein the gluing nozzle (1) is provided with a flow (14) by which the gluing Impurities are removed from the nozzle (1). The device according to the invention is characterized in that a suction device (7, 8, 17, 24, 30) is provided, by means of which the liquid is sucked away after the at least one gluing nozzle has been supplied with liquid. The method according to the invention is characterized in that the liquid (3, 14) and the removed impurities are sucked away from a region (20) close to the gluing nozzle (1).

A vacuum treatment method for dephosphorizing molten steel to produce low-phosphorus (or ultralow-phosphorus) weakly-deoxidized steel or other steel features that in the vacuum carbon-oxygen reactionphase, the oxygen or iron oxide needed by dephosphorizing reaction is added to the molten steel pool in a vacuum chamber while the lime blocks or powder is added for quickly dephosphorizing, and thismethod can also prevent rephosphorization from the steel ladle slag.

The invention discloses a CPA primer, kit and detection method for escherichia coli O157:H7. The CPA primer designed for a target rfbE comprises stripping primers 4s and 5a, a crossing amplification primer 2a1s and specific primers 2a and 3a; the nucleotide sequences of the primers are shown as SEQ ID NO.1-NO.5 respectively. A crossing priming amplification reaction detection and identification system designed for specific target sequences rfbE and stx1 of E.coli O157:H7 overcomes the defects of long required cycle, low sensitivity, high cost and difficult field application of methods in the prior art. By selecting a conserved region of the specific sequences rfbE and stx1 of a target strain, a pair of stripping primers, the crossing primer and the specific primers are designed to construct the crossing priming amplification reaction system, and a detection result is obtained within about 60 minutes to shorten the traditional cycle for detecting escherichia coli.

The invention discloses salmonella enteritidis detection reagent kit and method based on a loop-mediated isothermal amplification technology, wherein two specific inner primers and two specific outer primers are designed by the reagent kit according to the six specific regions of a salmonella enteritidis Sdf I gene conserved region, thereby ensuring the specificity of loop-mediated isothermal amplification and the reliability of a detecting result; in the invention, salmonella enteritidis is detected on the basis of the loop-mediated isothermal amplification technology, a target sequence can be rapidly, efficiently and specifically amplified under an isothermal condition, the operation is simple and convenient, expensive instruments and reagents are not needed, an amplified product is directly developed through fluorescent dye, a result can be judged by naked eyes, and the detection cost is low; and the reagent kit and the method can specifically distinguish serum specific type salmonella enteritidis and other serum type salmonella and are particularly suitable for detection and application in medium and small units and fields.

A reconfigurable leak testing system is provided that includes a leak testing station and at least one off-shuttle cart removably attachable to the leak testing station. A fixture cartridge is translatable between the off-shuttle cart and the leak testing station. The fixture cartridge is configured to selectively couple with the leak testing station. The fixture cartridge includes an upper fixture, a lower fixture, and an actuatable testing feature. The fixture cartridge is configured to receive a part between the upper fixture and lower fixture. The actuatable testing feature is configured to contact the part. A closed volume is defined at least partially by the actuatable testing feature and the part.

The invention discloses primers, kit and method for detecting Escherichia coli Shiga toxin I by PSR (polymerase spiral reaction). The primers for detecting Escherichia coli Shiga toxin I are designedaccording to specific target sequence stx1 of Shiga toxin I and include detection primer Ft and detection primer Bt, as well as acceleration primer IF and acceleration primer IB, and their nucleotidesequences are shown as SEQ ID NO. 1 to 4. The invention also provides a kit for detecting Escherichia coli Shiga toxin I by PSR; the kit includes the above primers, Bst DNA polymerase, and a mixed solution of calcein and manganese chloride, is suitable for detecting Escherichia coli Shiga toxin I by polymerase spiral reaction; after developing with a fluorescent dye, the results can be judged witheyes. The kit is simple and quick to operate, low in detection cost and suitable for field detection.

The invention discloses CPA primers and a detection kit for detection of methicillin-resistant staphylococcus aureus, and a detection method. The CPA primers are designed for two targets femA and mecAand comprise a stripping primer 4s and 5a, a cross-amplification primer 2a1s, and a specific primer 2a and 3a, wherein the primers have the sequences shown in SEQ ID NO.1-SEQ ID NO.10. The detectionmethod comprises the steps: establishing a cross-primer constant temperature amplification reaction system for detecting femA and mecA, carrying out cross-primer constant temperature amplification reaction, observing the color changes of the two reaction systems, and if colors of both the reaction systems turn into green, indicating that a sample to be tested contains methicillin-resistant staphylococcus aureus; otherwise, indicating that the sample to be tested does not contain methicillin-resistant staphylococcus aureus. The detection time of the primers and method is fast, and the detectionresult can be obtained in about 60 min. In addition, the detection sensitivity is high and reaches the level of f g / [mu]L.

The invention discloses primers, kit and method for detecting Vibrio parahemolyticus by PSR (polymerase spiral reaction) isothermal amplification reaction. A pair of detection primers Ft / Bt and a pairof acceleration primers IF / IB are designed according to specific target sequence tlh of Vibrio parahemolyticus, and their nucleotide sequences are shown as SEQ ID NO. 1 to 4; the primers ensure thatdetection results are reliable. The invention also provides a kit for detecting Vibrio parahemolyticus by PSR isothermal amplification reaction; the kit has high sensitivity, good specificity, good operational convenience, high operational speed, good result accuracy and reliability and low detection cost, and is suitable for application in small- to medium-sized units and field detection. The kitused herein to detect Listeria monocytogenes by polymerase spiral reaction; the method herein never causes time loss due to temperature changes, has low time consumption, the amplified product has noneed for gel electrophoresis, and after developing with a fluorescent dye, the results can be judged with eyes.

The invention discloses goose parvovirus detection kit and method based on a loop-mediated isothermal amplification technology. Based on six specific regions of a VP3 gene conserved region of a goose parvovirus, two specific primers and two specific outer primers are designed in the kit so as to ensure the high specificity and the reliability of a detection result of loop-mediated isothermal amplification. The invention detects the goose parvovirus on the basis of the loop-mediated isothermal amplification technology, can amplify a target sequence rapidly, efficiently and specifically under the isothermal condition, has simple and convenient operation and does not use expensive instruments and reagents; an amplification product can be developed directly by using a fluorescent dye and a result can be judged with naked eyes; the detection cost is low; and the invention is particularly suitable for small and medium size units and field tests.

The invention discloses a CPA detection primer, a kit and a method for pseudomonas aeruginosa. The CPA detection primer is designed for a target oprL, and has nucleotide sequences as shown in SEQ ID NO. 1-5. The invention also provides a CPA detection kit for the pseudomonas aeruginosa. The CPA detection kit has the advantages of high sensitivity, good specificity, good applicability, simple, convenient and rapid operation, accurate and reliable result, low detection cost and the like. The CPA detection primer or the CPA detection kit is utilized for cross a primer isothermal amplification reaction on a to-be-detected sample; results can be directly judged and read by naked eyes through fluorescent dye color development; and whether the to-be-detected sample contains the pseudomonas aeruginosa or not can be quickly and accurately detected, which has important significance for improving the disease diagnosis rate of important groups and diagnosing diseases in an early stage.

The invention discloses primers, kit and method for detecting methicillin-resistant Staphylococcus aureus by PSR (polymerase spiral reaction). The primers provided herein are shown as SEQ ID NO. 1 to4 and suitable for detecting methicillin-resistant Staphylococcus aureus by PSR; specificity, sensitivity and reliability can be detected for methicillin-resistant Staphylococcus aureus by detecting specific target sequences femA and mecA. The invention also provides a kit and method for detecting methicillin-resistant Staphylococcus aureus by PSR based on the primers; the method has the advantages of high sensitivity, good specificity, good operational convenience, high operational speed, accurate and reliable results, low detection cost and suitability to field detection; no special expensive instruments or agents are required; after developing with a fluorescent dye, the results can be judged with eyes; the kit and method are particularly suitable for detection in small- and medium-sized units and field detection and have a promising application prospect.

The invention relates to application of astragalus polysaccharides in loop-mediated isothermal amplification, a loop-mediated isothermal amplification detection kit containing the astragalus polysaccharides and a detection method. The application of the astragalus polysaccharides in the loop-mediated isothermal amplification, the loop-mediated isothermal amplification detection kit containing the astragalus polysaccharides and the detection method have the advantages that the astragalus polysaccharides are added into a loop-mediated isothermal amplification system until final concentration is 0.1-0.5mol / L (based on the weight of glucose), so that the stability of polymerase is increased, expensive 'polymerase' and 'betaine' in common LAMP (loop-mediated isothermal amplification) kits can be partially replaced, the amplification of high-content GC fragments can be well promoted, detection reaction time can be reduced to 20-30 minutes, detection cost can be lowered while the detection period can be shortened, and the application is significant to the loop-mediated isothermal amplification in pathogenic microorganism detection.

The invention discloses a primer, a kit and a method for PSR detection of heat-resisting direct hematoxin and heat-resisting related hematoxin. The invention respectively designs a pair of detection primers for specific zones of specific target sequence tdh and trh conserved domains of positive vibrio parahaemolyticus of heat-resisting direct hematoxin; the nucleotide sequences thereof are shown as SEQ ID NO.1-4; the primers are capable of quickly and accurately detecting vibrio parahaemolyticus heat-resisting direct hematoxin and heat-resisting related hematoxin and are high in applicability.The invention also provides the kit for PSR detection of heat-resisting direct hematoxin and heat-resisting related hematoxin. When the kit is utilized to detect a to-be-detected sample, a color developing agent can be utilized to directly judge a result with naked eyes; the operation is simple and convenient; the result is accurate; the detection cost is low.

The invention provides a method for transferring workpieces to enter and exit a vacuum environment and ensuring the workpieces to pass through a processing region in the vacuum environment. Before the workpieces are processed in the vacuum environment, a front buffer program is executed in a vacuum processing cavity. The program comprises the following steps of: carrying out one-by-one workpiece descending motion on one column of N workpieces; transferring the lowermost workpiece onto a horizontal transmission platform; and transferring the workpieces to the processing region one by one by the horizontal transmission platform to carry out processing. The invention also provides equipment for implementing the method, which comprises the vacuum processing cavity. The vacuum processing cavity is divided into a front buffer region, the processing region and a rear buffer region which are sequentially arranged; the horizontal transmission platform runs through the front buffer region, the processing region and the rear buffer region in the vacuum processing cavity; and lifting devices are arranged in the front buffer region and the rear buffer region. The method and the equipment have the advantages that in the workpiece transmission process, the workpieces always take the linear motion without turning; the transmission efficiency is high; the motion mode is simple; the design is also simple; a small number of mechanical parts are required; the cost is low; and the reliability is high.

In order to further develop a data processing device (100; 100') having at least one microprocessor (90) and having at least one additional arithmetic unit (40) as well as a method of performing at least one particular defined calculation by means of the data processing device (100; 100') in such a way that a plurality of calculations may be performed in sequence without any intervention by the microprocessor (90), it is proposed that the registers be loadable from at least one in particular peripheral memory (10), for example from at least one R[andom]A[ccess]M[emory], from at least one R[ead]O[nly]M[emory] or from at least one E[lectrically] E[rasable] P[rogrammable] R[ead]O[nly]M[emory.

The invention relates to an application of astragalus polysaccharide in loop-mediated isothermal amplification as well as a kit and a detection method of the astragalus polysaccharide. By adding the astragalus polysaccharide to a loop-mediated isothermal amplification system until a final concentration is 0.1-0.5mol / L (in terms of glucose), the stability of polymerase can be enhanced; expensive 'polymerase' and 'glycine betaine' in normal LAMP kits can be partially substituted; the amplification of a high-content GC segment can be promoted well; the reaction time of detection can be shortened to 20-30min; detection cost can be reduced, and meanwhile, a detection cycle can be shortened; and the astragalus polysaccharide has important significance for detection of pathogenic microorganisms through loop-mediated isothermal amplification.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection kit of vibrio parahaemolyticus and a detection method thereof and specifically relates to a group of primers for detecting the vibrio parahaemolyticus, which have oligonucleotide sequences shown in SEQ ID No. 1 to SEQ ID No. 6 in a sequence table, as well as the kit containing the primers and the detection method thereof. The kit disclosed by the invention is high in sensitivity, strong in specificity, low in cost and simple and convenient to operate.

The invention discloses a CPA detection primer, a detection kit and a detection method of salmonella. The CPA primer designed aiming at a target invA comprises stripping primers 4s and 5a, a cross amplification primer 2a1s and specific primers 2a and 3a; the nucleotide sequences of the primers are respectively as shown in SEQ ID NO.1 to SEQ ID NO.5. The cross primer isothermal amplification reaction detection and identification system designed for the salmonella specific target sequence invA provided by the invention overcomes the defects of long period, low sensitivity, high cost, difficulty in field application and the like of the method in the prior art. By selecting a conserved region of a specific sequence invA of a target strain, a pair of stripping primers, a cross primer and a specific primer are designed to construct a cross primer isothermal amplification reaction system, and a detection result can be obtained within about 60 minutes so as to shorten the traditional salmonella detection period.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection kit of vibrio cholerae and a detection method thereof and specifically relates to a group of primers for detecting the vibrio cholerae, which have oligonucleotide sequences shown in SEQ ID No. 1 to SEQ ID No. 6 in a sequence table, as well as the kit containing the primers and the detection method thereof. The kit disclosed by the invention is high in sensitivity, strong in specificity, low in cost and simple and convenient to operate.

In a method at a spinning preparation machine, for example a cleaner, opener, carding machine or the like, for cleaning fibre material, especially cotton, an examination of the nature of the trash iscarried out, which examination is used for adjustment of at least one adjustable cleaning element, for example a separating blade, cleaning grid or the like. In order to make possible improved and undisrupted production by simple means, the optimum adjustment of the at least one cleaning element for a specific fibre batch is stored in a memory of an electronic control and regulation device and, when the same fibre batch is processed again, the optimum adjustment of the cleaning element is implemented automatically.

The invention discloses salmonella enteritidis detection reagent kit and method based on a loop-mediated isothermal amplification technology, wherein two specific inner primers and two specific outer primers are designed by the reagent kit according to the six specific regions of a salmonella enteritidis Sdf I gene conserved region, thereby ensuring the specificity of loop-mediated isothermal amplification and the reliability of a detecting result; in the invention, salmonella enteritidis is detected on the basis of the loop-mediated isothermal amplification technology, a target sequence can be rapidly, efficiently and specifically amplified under an isothermal condition, the operation is simple and convenient, expensive instruments and reagents are not needed, an amplified product is directly developed through fluorescent dye, a result can be judged by naked eyes, and the detection cost is low; and the reagent kit and the method can specifically distinguish serum specific type salmonella enteritidis and other serum type salmonella and are particularly suitable for detection and application in medium and small units and fields.

The invention relates to a loop-mediated isothermal amplification kit for detecting chicken rhinitis haemophilus paragallinarum and a detection method of the loop-mediated isothermal amplification kit. The loop-mediated isothermal amplification kit for detecting the chicken rhinitis haemophilus paragallinarum and the detection method of the loop-mediated isothermal amplification kit have the advantages that dextran and astragalus polysaccharides are added into a loop-mediated isothermal amplification system until final concentration is 0.1-0.5mol / L (based on the weight of glucose), so that the stability of polymerase is increased, expensive 'polymerase' and 'betaine' in common LAMP (loop-mediated isothermal amplification) kits can be partially replaced, the amplification of high-content GC fragments can be well promoted, detection reaction time can be reduced to 20-30 minutes, detection cost can be lowered while the detection period can be shortened, and the kit is significant to the loop-mediated isothermal amplification in chicken rhinitis haemophilus paragallinarum detection.

The invention discloses primers, kit and method for detecting methicillin-resistant Staphylococcus aureus by PSR (polymerase spiral reaction). The primers provided herein are shown as SEQ ID NO. 1 to4 and suitable for detecting methicillin-resistant Staphylococcus aureus by PSR; specificity, sensitivity and reliability can be detected for methicillin-resistant Staphylococcus aureus by detecting specific target sequences femA and mecA. The invention also provides a kit and method for detecting methicillin-resistant Staphylococcus aureus by PSR based on the primers; the method has the advantages of high sensitivity, good specificity, good operational convenience, high operational speed, accurate and reliable results, low detection cost and suitability to field detection; no special expensive instruments or agents are required; after developing with a fluorescent dye, the results can be judged with eyes; the kit and method are particularly suitable for detection in small- and medium-sized units and field detection and have a promising application prospect.

The invention discloses CPA detection primers, a detection kit and a detection method for enterotoxin B-producing staphylococcus aureus. The CPA primers comprise stripping primers 4s and 5a, a cross amplification primer 2a1s, and specific primers 2a and 3a, wherein the nucleotide sequences of the above primers are respectively as shown in SEQ ID NO. 1 to SEQ ID NO. 5. A cross primer isothermal amplification reaction detection and identification system designed for the gene sequence of staphylococcus aureus enterotoxin B in by the invention overcomes the defects of long period, low sensitivity, high cost, difficulty in field application and the like of methods in the prior art. By selecting a conserved region of an enterotoxin B gene sequence of a target strain, a pair of the stripping primers, the cross primer and the specific primers are designed to construct a cross primer isothermal amplification reaction system, and a detection result is obtained within about 60 minutes, so the period of detecting enterotoxin B-producing staphylococcus aureus in the prior art is shortened.

The invention relates to a kit for detecting chicken coryza haemophilus paragallinarum on the basis of loop-mediated isothermal amplification and a detection method of the kit. By adding dextran and astragalus polysaccharide in a loop-mediated isothermal amplification system to reach the final concentration being 0.1-0.5 mol / L (on the basis of glucose), the stability of polymerase is improved, polymerase and betaine which are high in price in an ordinary LAMP kit can be partially replaced, amplification of high content GC fragments can be well promoted, the detection reaction time can be shortened to 20-30 minutes, and the detection period can be shortened while the detection cost is lowered; an important meaning to loop-mediated isothermal amplification on chicken coryza haemophilus paragallinarum detection is achieved.

The invention discloses a primer, a kit and a method for detecting Escherichia coli type I Shiga toxin by PSR. The present invention designs primers for detecting Escherichia coli type I Shiga toxin for the heterosexual target sequence stx1 of type I Shiga toxin, and the primers include detection primer Ft and detection primer Bt, acceleration primer IF and acceleration primer IB, and its nucleotide The sequences are shown as SEQ ID NO.1-SEQ ID NO.4. The present invention also provides a kit for detecting Escherichia coli type I Shiga toxin by PSR, comprising the above-mentioned primers, Bst DNA polymerase, and a mixed solution of calcein and manganese chloride, capable of detecting Escherichia coli type I Shiga toxin For the detection of polymerase helical reaction, the result can be judged by the naked eye by directly using fluorescent dyes to develop color. The operation is simple and fast, and the detection cost is low, which is suitable for on-site detection applications.

The invention discloses goose parvovirus detection kit and method based on a loop-mediated isothermal amplification technology. Based on six specific regions of a VP3 gene conserved region of a goose parvovirus, two specific primers and two specific outer primers are designed in the kit so as to ensure the high specificity and the reliability of a detection result of loop-mediated isothermal amplification. The invention detects the goose parvovirus on the basis of the loop-mediated isothermal amplification technology, can amplify a target sequence rapidly, efficiently and specifically under the isothermal condition, has simple and convenient operation and does not use expensive instruments and reagents; an amplification product can be developed directly by using a fluorescent dye and a result can be judged with naked eyes; the detection cost is low; and the invention is particularly suitable for small and medium size units and field tests.