Application of astragalus polysaccharide in loop-mediated isothermal amplification as well as kit and detection method of astragalus polysaccharide

Abstract

The invention relates to an application of astragalus polysaccharide in loop-mediated isothermal amplification as well as a kit and a detection method of the astragalus polysaccharide. By adding the astragalus polysaccharide to a loop-mediated isothermal amplification system until a final concentration is 0.1-0.5mol / L (in terms of glucose), the stability of polymerase can be enhanced; expensive 'polymerase' and 'glycine betaine' in normal LAMP kits can be partially substituted; the amplification of a high-content GC segment can be promoted well; the reaction time of detection can be shortened to 20-30min; detection cost can be reduced, and meanwhile, a detection cycle can be shortened; and the astragalus polysaccharide has important significance for detection of pathogenic microorganisms through loop-mediated isothermal amplification.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the application of astragalus polysaccharide in ring-mediated isothermal amplification, and also relates to a kit containing astragalus polysaccharide and a detection method. Background technique [0002] At present, the detection methods of original microorganisms mainly include isolation and culture identification, polymerase chain reaction (PCR) and fluorescence quantitative PCR (FQ-PCR). The method requires expensive instruments and reagents, and the detection cost is high, so it is not suitable for small and medium-sized units and on-site detection applications. [0003] Compared with other nucleic acid amplification techniques, loop-mediated isothermal amplification (LAMP) technology can rapidly, efficiently and specifically amplify target sequences under isothermal conditions, and is easy to operate without expensive instruments And reagents, low cost, has shown broad development p...

AI Technical Summary

Problems solved by technology

Once Haemophilus paragallinitis infection occurs in chicken flocks in a certain area, the disease will become an endemic disease, which is difficult to eradicate completely, and often recurs, causing huge economic losses

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