Application of astragalus polysaccharide in loop-mediated isothermal amplification, loop-mediated isothermal amplification detection kit containing astragalus polysaccharides and detection method

Abstract

The invention relates to application of astragalus polysaccharides in loop-mediated isothermal amplification, a loop-mediated isothermal amplification detection kit containing the astragalus polysaccharides and a detection method. The application of the astragalus polysaccharides in the loop-mediated isothermal amplification, the loop-mediated isothermal amplification detection kit containing the astragalus polysaccharides and the detection method have the advantages that the astragalus polysaccharides are added into a loop-mediated isothermal amplification system until final concentration is 0.1-0.5mol / L (based on the weight of glucose), so that the stability of polymerase is increased, expensive 'polymerase' and 'betaine' in common LAMP (loop-mediated isothermal amplification) kits can be partially replaced, the amplification of high-content GC fragments can be well promoted, detection reaction time can be reduced to 20-30 minutes, detection cost can be lowered while the detection period can be shortened, and the application is significant to the loop-mediated isothermal amplification in pathogenic microorganism detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the application of astragalus polysaccharide in ring-mediated isothermal amplification, and also relates to a kit containing astragalus polysaccharide and a detection method. Background technique [0002] At present, the detection methods of original microorganisms mainly include isolation and culture identification, polymerase chain reaction (PCR) and fluorescence quantitative PCR (FQ-PCR). The method requires expensive instruments and reagents, and the detection cost is high, so it is not suitable for small and medium-sized units and on-site detection applications. [0003] Compared with other nucleic acid amplification techniques, loop-mediated isothermal amplification (LAMP) technology can rapidly, efficiently and specifically amplify target sequences under isothermal conditions, and is easy to operate without expensive instruments And reagents, low cost, has shown broad development p...

AI Technical Summary

Problems solved by technology

Once Klebsiella gallinarum infection occurs in chicken flocks in a certain area, the disease will become an endemic disease, it is difficult to completely eradicate, and frequent repeated outbreaks will bring huge economic losses

The main pathological features of Klebsiella chicken rhinitidis infection are chronic atrophic rhinitis, stench, sepsis, and urinary tract infection. It is difficult to distinguish it from Haemophilus paragallinarum infection pathologically.

Images