Primer, kit and method for PSR detection of heat-resisting direct hematoxin and heat-resisting related hematoxin

Abstract

The invention discloses a primer, a kit and a method for PSR detection of heat-resisting direct hematoxin and heat-resisting related hematoxin. The invention respectively designs a pair of detection primers for specific zones of specific target sequence tdh and trh conserved domains of positive vibrio parahaemolyticus of heat-resisting direct hematoxin; the nucleotide sequences thereof are shown as SEQ ID NO.1-4; the primers are capable of quickly and accurately detecting vibrio parahaemolyticus heat-resisting direct hematoxin and heat-resisting related hematoxin and are high in applicability.The invention also provides the kit for PSR detection of heat-resisting direct hematoxin and heat-resisting related hematoxin. When the kit is utilized to detect a to-be-detected sample, a color developing agent can be utilized to directly judge a result with naked eyes; the operation is simple and convenient; the result is accurate; the detection cost is low.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a primer, a kit and a method for detecting heat-resistant direct hemolytic toxin and heat-resistant related hemolytic toxin by PSR. Background technique [0002] Vibrio parahaemolyticus is an important food-borne pathogen. After people eat food contaminated by this bacteria, it will cause symptoms such as diarrhea and vomiting, and even lead to dehydration, shock and death. At present, the scale of food poisoning caused by Vibrio parahaemolyticus is obviously expanding. Thermostable direct hemolysin (TDH) or thermostable related hemolysin (TRH) is one of the virulence factors of Vibrio parahaemolyticus, which is the most toxic and can cause red blood cell hemolysis and damage the cell membrane. and lysosomes. [0003] At present, the detection of TDH and TRH mainly includes the traditional disk method, the PCR method based on molecular biotechnology, the detection metho...

AI Technical Summary

Problems solved by technology

The workload of conventional detection and identification methods is relatively large, and the detection results take a long time, and the identification results need to be judged according to the experimental phenomena, which is difficult to meet the needs of rapid identification

The gene chip detection and PCR amplification methods developed in recent years have strong specificity, rapidity and sensitivity, but the cost is relatively high

Immunological detection methods, such as ELISA, immunochromatography, etc., have high sensitivity, but the operation process is complicated and the cost is high, so it is not suitable for large-scale detection of samples

At present, the most widely used constant temperature amplification technology (LAMP) also has its limitations, such as high cost, long time-consuming, unstable and easy to pollute, etc.

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