Kit for detecting chicken coryza type Klebsiella on basis of loop-mediated isothermal amplification and detection method of kit

Abstract

The invention relates to a kit for detecting chicken coryza type Klebsiella on the basis of loop-mediated isothermal amplification and a detection method of the kit. By adding dextran and astragalus polysaccharide in a loop-mediated isothermal amplification system to reach the final concentration being 0.05-0.25 mol/L, the stability of polymerase is improved, betaine high in price in an ordinary LAMP kit can be partially replaced, amplification of high content GC fragments can be well promoted, the detection reaction time can be shortened to 20-30 minutes, and the detection period can be shortened while the detection cost is lowered; an important meaning to loop-mediated isothermal amplification in pathogenic microorganism detection is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to a kit for detecting Klebsiella gallinarum based on loop-mediated isothermal amplification, and also relates to a method for detecting Klebsiella gallinarum by using the kit. Background technique [0002] At present, the detection methods of original microorganisms mainly include isolation and culture identification, polymerase chain reaction (PCR) and fluorescence quantitative PCR (FQ-PCR). The method requires expensive instruments and reagents, and the detection cost is high, so it is not suitable for small and medium-sized units and on-site detection applications. [0003] Compared with other nucleic acid amplification techniques, loop-mediated isothermal amplification (LAMP) technology can rapidly, efficiently and specifically amplify target sequences under isothermal conditions, and is easy to operate without expensive instruments And reagents, low cost, has shown broad development pr...

AI Technical Summary

Problems solved by technology

Once Klebsiella gallinarum infection occurs in chicken flocks in a certain area, the disease will become an endemic disease, it is difficult to completely eradicate, and frequent repeated outbreaks will bring huge economic losses

The main pathological features of Klebsiella chicken rhinitidis infection are chronic atrophic rhinitis, stench, sepsis, and urinary tract infection. It is difficult to distinguish it from Haemophilus paragallinarum infection pathologically.

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