50results about How to "Mild purification conditions" patented technology

The invention discloses a method for preparing high-purity cyclic hexapeptide compound by utilizing Coleophoma empetri to ferment a metabolite. The method comprises the steps of conducting filtration on FR179642 fermentation broth, concentrating the filter liquor, and through the steps of adsorption by macroporous adsorption resin, desorption, desorption solution decoloration, lower temperature crystallization and the like, the high-purity cyclic hexapeptide compound FR179642 product is obtained. The high-purity cyclic hexapeptide compound FR179642 has the advantages that the high-purity cyclic hexapeptide compound FR179642 is extracted by adopting macroporous adsorption resin and combining a method of using a decolorising agent and crystallization, the technology is easy to operate and applicable to industrial production.

The invention discloses a preparation method of high-purity spirulina blue. The preparation method is characterized by adopting spirulina powder as a raw material, and performing continuous productionincluding extraction, filtration, salting out, desalination concentration, micro-encapsulation, spray drying and mixing to obtain spirulina blue, which is blue powder. Phycocyanin is dark blue powder, is separated from spirulina, and looks beautiful. The main component of spirulina blue is phycocyanin, which belongs to porphyrin cyanophycin and is one of infrequent chromoproteins in the nature. The phycocyanin has bright color, is protein itself with abundant nutrients, has complete amino acid composition, and is high in amino acid content. The preparation method is short in production periodand doesn't cause secondary pollution. The obtained spirulina blue is high in purity, contains 71.3% of phycocyanin, has the purity A620 / A280 reach 3.6, and can meet the requirement of food and cosmetic industry.

The invention relates to an A1S-1462 recombinant protein and a preparation method and application thereof. The recombinant protein comprises A1S-1462 mature peptide, and the amino acid sequence of the recombinant protein is shown as SEQ ID NO. 3. The recombinant protein is high in expression amount, easy to separate and purify, efficient and safe, can be directly used with an adjuvant, and is used for the preparation of an acinetobacter baumannii infection resistant subunit vaccine and a related detection kit. Confirmed by animal experiments, the genetic engineering recombinant subunit vaccine has good acinetobacter baumannii infection resistant immune protection effect, lays a foundation for the further study on combined vaccines and multicomponent fusion vaccines, and plays an important role for development and application of prevention and control vaccines and diagnostic kits.

The invention belongs to the technical field of bacterial antigens, and discloses a recombinant protein SBP of Pseudomonas aeruginosa vaccine and its preparation method and application; the nucleotide sequence is SEQ ID NO: 1; the amino acid sequence is SEQ ID NO: 2; the recombinant The expression vector comprises the nucleotide sequence of SEQ ID NO:1. The present invention adopts pGEX-6p-1 carrier to construct recombinant expression plasmid, expresses recombinant protein SBP, pGEX is the carrier expressing fusion protein constructed by Smith and Johnson in 1987, and its main feature is that the carrier is connected with a molecular weight of 26kDa Glutathione‑S‑transferase (GST), the expressed fusion protein contains a GST tag, which is a marker for protein purification. Compared with other fusion vectors, the pGEX series vectors can keep the purified protein in its spatial conformation and immunogenicity to the greatest extent.

The invention relates to a system and method for extracting sulfur from sulfur-containing foam in a coking plant. The system includes an extraction unit, a separation unit and a post-processing unit; the extraction unit includes an extraction tower, and the extraction tower includes a tower body and a motor. The tower body is from top to bottom. The lower section includes an upper clarification section, a mixing section and a lower clarification section in turn. The upper clarification section is provided with a raffinate phase outlet, the lower clarification section is provided with an extraction phase outlet, and the top and bottom of the mixing section are respectively provided with an extractant inlet. and a sulfur-containing foam feed port; the separation unit includes a thermal insulation filter device, and the feed port is connected to the extraction phase discharge port; the post-processing unit includes an evaporative crystallizer, and the still body of the evaporative crystallizer is provided with a material inlet, and a vapor phase outlet The material inlet is connected with the material outlet, the material inlet is connected with the filtrate outlet of the thermal insulation filter device, the vapor phase outlet is connected with the extractant inlet of the extraction tower, and the material outlet is used to obtain sulfur. The invention directly separates useful components such as sulfur and ammonium salt from sulfur-containing foam, and is economical and environment-friendly.

The invention discloses a recombinant fusion protein HF2 which is composed of staphylococcus aureus alpha hemolysin (H1a) and a fibronectin binding protein (FNBA) segment, wherein the H1a and the FNBA are fused through a linker peptide. The protein can be used for preparing subunit vaccines and relevant detection kits capable of resisting infections of the methicillin-resistant staphylococcus aureus (MRSA). A gene engineering technology is used to recombine and express the fused protein. The method has high expression level, facilitates separation and purification and has high efficiency and safety. Animal experiment results show that the recombinant fusion protein HF2 can effectively stimulate a body to produce relatively high humoral immune response and good immune protection effect.

The invention provides PA (pseudomonas aeruginosa) recombinant protein POP, as well as a preparation method and an application thereof. The recombinant protein is formed by sequential connection of a PA III type secretory system component protein PcrV fragment, a PA outer membrane lipoprotein OprI fragment and a PA IV type pilin PilA fragment through a linker. The recombinant protein has a general formula: PcrV fragment-(Linker A)m-Oprl fragment-(Linker B)n-PilA fragment, wherein sequences of the Linker A and the Linker B are respectively independently selected from one of GGGGS, GGSGG and YAPVDV; values allowed for m and n are 1, 2, 3 and 4 independently and respectively. The invention also provides the preparation method and the application of the recombinant protein. Subunit vaccine prepared from the recombinant protein can stimulate the organism to generate high-titer IgG antibodies; the recombinant protein can be used for preparing diagnostic drugs, preventive drugs or therapeutic drugs.

The present invention relates to a recombinant protein of 1A1S_1969 and its preparation method and application. The recombinant protein comprises the 46-414th amino acid sequence of A1S_1969. The amino acid sequence of the recombinant protein is shown in SEQ ID NO.5. The recombinant protein has a high expression level, is convenient for separation and purification, is highly efficient and safe, and can be directly used in conjunction with an adjuvant to prepare subunit vaccines against Acinetobacter baumannii infection and related detection kits. It is confirmed by animal experiments that the genetically engineered recombinant monovalent subunit vaccine has a good immune protection effect against Acinetobacter baumannii infection. It lays the foundation for further research on combined vaccines and multi-subunit fusion vaccines, and plays an important role in the development and application of prevention and control vaccines and diagnostic kits.