The invention discloses an oyster mushroom cultivation method. The method includes the following steps of 1, culture medium preparation, 2, packing, 3, sterilization, 4, cooling, 5, inoculation, 6, fungus cultivation, 7, mushroom growth and 8, harvesting. The oyster mushroom cultivation method has the advantages that adopted raw materials are pollution free and nutrient-rich, and chemicals such as chemical fertilizer, pesticide and disinfector are not used in the process of cultivation, so that harm to human bodies due to the chemicals is avoided, the problem about environmental pollution is solved under the premise that population health is guaranteed, and nutritive value of pleurotuseryngii is also guaranteed; besides, fruiting conversion rate is increased obviously as well, and further fruiting yield is increased.