The invention relates to the technical field of biology, in particular to a rapid, simple and low-cost kit for screening urinary tract infection pathogenic bacteria and application of the kit, the kit comprises primers as shown in sequences SEQ ID NO: 1-12, and a proper PCR reaction system, reaction conditions and probes as shown in sequences SEQ ID NO: 13-18 are recorded. The multiplex PCR detection method and the kit for screening the urinary tract infection pathogenic bacteria are reliable and rapid in result and low in cost, and experimental results show that the detection method and the kit are high in recognition speed on the urinary tract infection pathogenic bacteria such as escherichia coli, klebsiella pneumoniae, staphylococcus saprophyticus, enterococcus faecalis, streptococcus agalactiae and proteus mirabilis, and the detection method and the kit can be used for screening the urinary tract infection pathogenic bacteria. The timeliness and the accuracy of pathogen identification are improved, so that the possibility is provided for improving public health and realizing precise medical treatment.