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Rapid, simple and low-cost kit for screening urinary tract infection pathogenic bacteria and application thereof

A technology for urinary tract infection and pathogenic bacteria, applied in the biological field, to achieve low cost, improve public health, improve timeliness and accuracy

Pending Publication Date: 2022-03-22
SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports about a quick, easy and low-cost screening kit for urinary tract infection pathogenic bacteria of the present invention and its application

Method used

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  • Rapid, simple and low-cost kit for screening urinary tract infection pathogenic bacteria and application thereof
  • Rapid, simple and low-cost kit for screening urinary tract infection pathogenic bacteria and application thereof
  • Rapid, simple and low-cost kit for screening urinary tract infection pathogenic bacteria and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] 1 detection method

[0051] 1.1 Sample pretreatment and nucleic acid extraction

[0052] Take the urine sample (1-2ml) to be tested in our hospital and centrifuge at 13000rpm for 10min, then remove the supernatant. The purpose of this step is to enrich the pathogenic microorganisms in the urine by centrifugation to increase the detection rate. Then use bacterial genomic DNA extraction kit (Tiangen, DP302) to extract nucleic acid according to the standard operation in the manual. Extracted nucleic acid products should be used immediately for downstream detection or stored at -20°C.

[0053] 1.2 Multiplex qPCR detection

[0054] Thaw all reagents first, mix by inversion and centrifuge briefly before use. Then prepare detection reaction solutions A and B for each sample according to the following table, with a total volume of 20ul. The reaction system is placed in an optical flat-cap PCR reaction tube, and kept at low temperature and protected from light; after the rea...

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Abstract

The invention relates to the technical field of biology, in particular to a rapid, simple and low-cost kit for screening urinary tract infection pathogenic bacteria and application of the kit, the kit comprises primers as shown in sequences SEQ ID NO: 1-12, and a proper PCR reaction system, reaction conditions and probes as shown in sequences SEQ ID NO: 13-18 are recorded. The multiplex PCR detection method and the kit for screening the urinary tract infection pathogenic bacteria are reliable and rapid in result and low in cost, and experimental results show that the detection method and the kit are high in recognition speed on the urinary tract infection pathogenic bacteria such as escherichia coli, klebsiella pneumoniae, staphylococcus saprophyticus, enterococcus faecalis, streptococcus agalactiae and proteus mirabilis, and the detection method and the kit can be used for screening the urinary tract infection pathogenic bacteria. The timeliness and the accuracy of pathogen identification are improved, so that the possibility is provided for improving public health and realizing precise medical treatment.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a fast, simple and low-cost kit for screening urinary tract infection pathogenic bacteria and its application. Background technique [0002] Urinary tract infection, also known as urinary tract infection, is a general term for infections in various parts of the urinary system such as the kidneys, ureters, bladder, and urethra caused by pathogenic microorganisms. Urinary tract infection is a common infectious disease. There are nearly 150 million UTIs in the world every year, and 40% to 50% of women have UTIs in their lifetime. 9.4% to 50% of nosocomial infections in my country come from urinary tract infections. Severe urinary tract infections, such as complicated urinary tract infections and pyelonephritis, can easily cause sepsis and even septic shock. The most common pathogens include Escherichia coli, Staphylococcus, Streptococcus, Enterococcus, and Klebsiella. [0003] The ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/689C12Q1/686C12R1/19C12R1/22C12R1/44C12R1/01C12R1/46C12R1/37
CPCC12Q1/689C12Q1/686C12Q2600/16C12Q2537/143C12Q2563/107C12Q2561/101
Inventor 赵醴单红梅刘建全
Owner SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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