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222results about How to "Improve marking effect" patented technology

Method and computer system for part-of-speech tagging of incomplete sentences

The invention relates to a method and a computer system for enhanced part-of-speech (POS-) tagging as well as grammatically disambiguating a phrase. A phrase is usually a short multiword expression that may be ambiguous. By introducing grammatical constraints the invention supports POS-tagging as well as grammatically disambiguating the phrase. According to an identifier for the phrase, the phrase is supplemented with artificial context information. The supplemented phrase is then POS-tagged or grammatically disambiguated. Important applications are POS-tagging, Automatic Term Encoding, Headword Detection and Information Retrieval.
Owner:XEROX CORP

Automatic image annotation method based on semi-supervised learning

InactiveCN107644235AHigh expressionEffective dimensionality reductionCharacter and pattern recognitionData setSupervised learning
The invention discloses an automatic image annotation method based on semi-supervised learning. The method comprises the steps that a data set is divided into a training data set, an unlabeled data set and a test set; the SIFT feature and the HOG feature of a training sample are extracted to train an LDA_SVM classifier; color and texture features are extracted to train a neural network; unlabeleddata are used to enable two classifiers to label and predict the same unlabeled sample simultaneously; according to the contribution of the classifiers to the unlabeled sample classification accuracy,the classification results of two classifiers are weighted and fused by an adaptive weighted fusion policy, so as to acquire the final prediction label probability vector of the sample; and finally two classifiers are updated by the sample with high confidence and the predictive label thereof until the preset maximum number of iterations is reached. According to the invention, the method can makefull use of the unlabeled sample to excavate the inherent law of the image feature; the number of annotation samples required for the classifier training is effectively reduced; the annotation effectis great.
Owner:GUANGXI NORMAL UNIV

Image automatic marking method based on Monte Carlo data balance

The present invention relates to an image automatic marking method based on Monte Carlo data balance. The method comprises the steps of carrying out the region segmentation on the training sample images in a public image library, enabling the segmented regions possessing different characteristic description to correspond to one marking word, then carrying out the Monte Carlo data balance on the different types of image sets, extracting the multiscale characteristics of the balanced images, and finally inputting the extracted characteristic vectors in a robustness least squares increment limit learning machine to carry out the classification training to obtain a classification model in the image automatic marking; for the to-be-marked images, carrying out the region segmentation on the to-be-marked images, adopting the same multiscale characteristic fusion extraction method and inputting the extracted characteristic vectors in the least squares increment limit learning machine to obtain a final image marking result. Compared with a conventional image automatic marking method, the method of the present invention enables the images to be marked more effectively, is strong in timeliness, can be used for the automatic marking of the large-scale images, and possesses the actual application meaning.
Owner:FUZHOU UNIV

Laser marked plastic and preparation method thereof

The invention relates to plastic which can be marked by laser, which comprises the following blended components in weight portion: 100 portions of plastic, 0.1 to 10 portions of powder rubber with the average grain diameter of between 50 and 200 nanometers and the gel content of over 60 weight percent, and 0.01 to 5 portions of pearlescent pigment or metallic oxide. The invention also relates to a method for preparing the plastic which can be marked by the laser. The preparation method comprises the steps of melting and blending the components comprising the plastic, the powder rubber and the pearlescent pigment to prepare the plastic which can be marked by the laser. The plastic which can be marked by the laser can obtain a laser mark with higher contrast grade under the action of the laser. The preparation method has the advantages of simple and reliable processes.
Owner:CHINA PETROLEUM & CHEM CORP +1

Polypropylene composition supporting laser marking and preparation method and application thereof

The invention discloses a polypropylene composition supporting laser marking and a preparation method and an application thereof. The polypropylene composition comprises the following components in percentage by mass: 40-80 percent of polypropylene resin, 5-30 percent of an elastomer, 0-40 percent of an inorganic filler, 0.01-5 percent of a marking agent and 0.01-5 percent of a white toner. The preparation method comprises the following steps of: proportionally mixing the components; melting, blending and extruding by using an extruder; performing water cooling and bracing; and pelletizing. Due to the adoption of the marking agent and the prepared polypropylene composition supporting laser marking, a clear deep-color mark can be formed under a light color background under the condition of a small adding amount of the marking agent, the mark is firm and durable and does not disappear due to mechanical, chemical and physical factors, the cost is relatively low, and the use requirements of plastic parts in the industries of automobiles, home appliances, IT (Internet Technology), office equipment, electric tools, packages and the like can be fully met.
Owner:KINGFA SCI & TECH CO LTD +2

Full-automatic wafer rear marking machine

The invention relates to a novel device for marking IC crystal, in particular to a full-automatic crystal back marking machine, which uses a mechanical hand to take out crystal from a material feeding box to be sent to a crystal direction checker, a crystal marking platform or the like, to be marked and checked to be taken off by a mechanical hand to be put into a material outputting box. The invention is composed of a front area and a back area, wherein the front area is arranged with an electric controller and a PC, a baffle plate is arranged between the front and back areas, the baffle plate is arranged with a gate entrance, the outer side of the front area is arranged with a material feeding box and a material discharging device, the front area is arranged with a mechanical hand and a crystal direction checker inside, the back area is arranged with a crystal marking platform inside, the marking platform is respectively arranged with a before-marking checker and an after-marking checker above and under.
Owner:GRAND TECH SHENZHEN

Method and device for automatic image labeling based on non-equal probability random search of directed graphs

The invention discloses an image automatic annotation method based on digraph unequal probability random search, which comprises the following steps: inputting an image to be annotated and an annotated image set; extracting a plurality of feature vectors of the image to be annotated; selecting an adjacent image set; constructing a digraph model of the image to be annotated; calculating a word similarity matrix Se between tags and a symbiotic relationship matrix Co between tags; fusing the word similarity matrix Se between tags and the symbiotic relationship matrix Co between tags, so as to obtain a tag similarity matrix TT; and carrying out unequal probability random search on each candidate tag in a candidate tag set in the digraph model, so as to calculate the score, and obtaining a plurality of high-score candidate tags to be used as the label results. The invention also discloses an image automatic annotation device based on digraph unequal probability random search. In the invention, the dependency relation between images and similarity relation between tags are utilized fully and reasonably, thus the image automatic annotation can be effectively carried out, and the annotation effect is better.
Owner:清软微视(杭州)科技有限公司

Halogen-free flame retardant polyester with laser marking function and preparation method thereof

The invention discloses halogen-free flame retardant polyester with the laser marking function and a preparation method thereof. The polyester provided by the invention comprises the following components in percentage by weight: 25-75% of polyester resin, 0-50% of alkali-free glass fiber, 10-35% of halogen-free flame retardant agent, 0.3-5% of laser marking agent, 0-20% of toughening agent, 0.5-1% of acid absorbing agent, 0.3-0.5% of oxidation resisting agent, 0.5-5% of coloring agent and 0.5-5% of processing aid. The invention also provides a preparation method of the halogen-free flame retardant polyester with the laser marking function, which comprises the following steps of: after uniformly mixing all the components according to the ratio, delivering the mixture into a double-screw extruder, and carrying out melting, mixing, extruding, strip stretching, cooling, drying and granulating the mixture through the extruder to obtain granules. The polyester provided by the invention has excellent electrical properties and mechanical properties, various colors and good laser marking effect. The preparation method provided by invention is simple and is applicable to continuous large-scale production.
Owner:KINGFA SCI & TECH CO LTD +1

Differential-lid-separating multi-head laser bottle lid flying marking device

The invention relates to a laser bottle lid flying marking device, in particular to a differential-lid-separating multi-head laser bottle lid flying marking device and belongs to the technical field of bottle lid laser marking equipment. The differential-lid-separating multi-head laser bottle lid flying marking device comprises a bottle lid differential separation conveying chain plate mechanism, a laser marking mechanism and a two-dimensional code photographing and code reading mechanism which are sequentially arranged; the bottle lid differential separation conveying chain plate mechanism comprises a bottle lid input mechanism, a bottle lid output mechanism and a bottle lid guiding mechanism; the bottle lid output mechanism is arranged at the tail end of the bottle lid input mechanism; the bottle lid guiding mechanism is located between the tail end of the bottle lid input mechanism and the front end of the bottle lid output mechanism; and the bottle lid guiding mechanism communicates with a conveying channel at the tail end of the bottle lid input mechanism and the front end of the bottle lid output mechanism. According to the differential-lid-separating multi-head laser bottle lid flying marking device, operation can be completed for bottle lids of different sizes and specifications just by adjusting bottle lid guiding plates of different widths, time is saved, and operation is convenient; a marking result is inspected through a photographing and code reading system, and the product marking effect is improved; and the energy consumption is low, and noise is small.
Owner:HUANGCHANG AUTOMATION ENG SHANGHAI CO LTD

Intelligent paper marking method for subjective questions

ActiveCN110413741ASolving inefficienciesSolve the problem of uneven marking qualityNeural architecturesText database queryingSemantic vectorTwo-vector
The invention provides an intelligent paper marking method for subjective questions. The method comprises the steps of respectively preprocessing an examinee answer text and a reference answer text toobtain word vector sequence representations corresponding to the examinee answer text and the reference answer text; extracting semantic vectors of sentences in the examinee answers and the referenceanswers by utilizing a semantic feature extraction network; calculating semantic matching degrees of sentence vectors of the examinee answers and the reference answers by utilizing a fusion attentionmechanism; calculating an examinee answer weighted sentence vector by utilizing the matching result; aiming at the sentence vector sequence of the answers of the complete examinees and a weighted sentence vector sequence based on a fusion attention mechanism; utilizing a semantic feature extraction network to calculate semantic vectors of the complete answers and semantic vectors based on attention of the reference answers; and bonding the two vectors to form a final vector representation of the examinee answer; scoring the final vector representation of the examinee answer by using a multi-layer feedforward neural network. According to the invention, automatic examination paper marking of subjective questions can be realized, and examination paper marking efficiency is greatly improved.
Owner:山东山大鸥玛软件股份有限公司

Auxiliary focusing mechanism of laser marking machine and use method thereof

The invention relates to an auxiliary focusing device for laser marking system and the application method; wherein, the device comprises two supports, and two light pens which can give off visible parallel beam and is connected with a controllable power. The supports capable of moving horizontally are arranged on the focus mirror bases at both side of the focus mirror and the two light pens are pivotally arranged on the two supports respectively. Spinning and translating locking devices are arranged on the supports and the side-to-side translation of the supports with the spinning of the light pen can adjust the beam locating on the worktable. The invention adopts the application method that: first regulating and adjusting the distance between the focus mirror and the worktable, which enables the laser beam to focus on the processing workpiece on the worktable and the strongest sparkle serves as the focal point, namely the center of the scanning area, then switching on the light pens and regulate the two beams to overlap on the sparkle on the processing workpiece and lock tightly. The regulation of the distance between the worktable and the focus mirror with the light pens coinciding on the intersection point of the optic axis of the focus mirror and the planar, and focusing and the working counter pointing are both convenient.
Owner:深圳市星辰智能控制有限公司 +1

Laser markable polyethylene composition and preparation method thereof

The invention relates to polyethylene composition which can be marked with laser and the preparation method thereof. The composition includes the following blended components: 100 parts of polyethylene by weight, 0.1-15 parts of powdered rubber by weight, 0-2 parts of laser additive by weight, wherein, the powdered rubber comprises butylbenzene powdered rubber or / and carboxyl styrene butadiene powdered rubber. The preparation method comprises the step that the components including the polyethylene and the powdered rubber is melted and blended according to the compounding ratio of the components. The composition of the invention can overcome the problems that the polyethylene is difficult to be marked with laser, and the material physical performance is decreased and the cost is high caused by overabundant addition amount of the prior laser additive. In the preparation method of the invention, the process is simple and reliable. The polyethylene composition which can be marked with laser in the invention can be widely applied to the polyethylene products which are needed to be marked with laser.
Owner:CHINA PETROLEUM & CHEM CORP +1

Immune fluorescent marking method for naked plant pollen tube microtubule skeleton and its use

An immune-fluorescence labeling method of micro-pipe frame at pollen tube on gymnosperm includes fixing pollen tube of gymnosperm on fixing liquid for 40-60min, washing pollen tube, placing pollen tube in enzyme solution for carrying out enzymolysis of 30-40 min under temperature of 20-30deg.c, washing pollen tube, setting pollen tube in Triton X-100 to penetrate there for 1-3h, washing pollen tube, adding anti-beta-tubulin single clone antibody to carry out hatching for 1-3h, washing pollen tube, adding fluorescence label to carry out shielded hatching for 1-3h for making micro-pipe frame be immune-fluorescence labeled.
Owner:INST OF BOTANY CHINESE ACAD OF SCI

Individual fluorescence labeling method for early-stage newborn green crabs to adult green crabs

The invention discloses an individual fluorescence labeling method for early-stage newborn green crabs to adult green crabs and relates to an individual labeling method used in aquiculture. The method comprises the following steps of: preparing a fluorescent colloid label and putting the fluorescent colloid label into a syringe for later use; before injecting the fluorescent colloid label, putting the green crabs in ice water with the temperature of between 0 and 4 DEG C to lower the activities of the green crabs; in the process of injecting the fluorescent colloid label, inserting a syringe needle into a part on which the head, chest and belly cortex of the green crab is jointed with muscle from the bottom end of an appendage of the green crab along a belly wall and extracting the syringe needle outwards to form a linear label; and after the fluorescent colloid label is injected, wiping the injected fluorescent colloid label remaining around a position where the syringe needle is inserted.
Owner:XIAMEN UNIV

Dynamic rotation marking control system and method

ActiveCN106994561ASimple structureHigh efficiency of rotary markingLaser beam welding apparatusGalvanometerMovement control
The invention relates to the technical field of laser processing, and discloses a dynamic rotation marking control system. According to the control system, an upper computer transmits control information to a motion control card, the motion control card outputs motion signals to a galvanometer assembly and a rotation shaft according to the control information, and the motion control card further outputs control signals to an optical fiber laser device and controls light emitting and light closing of the optical fiber laser device; the optical fiber laser device emits a laser according to the control signals and outputs the laser to the galvanometer assembly; the galvanometer assembly controls rotation of a reflection lens adopted in the galvanometer assembly according to the motion signals; the received laser further acts on a workpiece through a focusing lens; the rotation shaft controls rotation of the workpiece according to the motion signals, and accordingly circumferential marking of the workpiece through the laser is achieved. According to the dynamic rotation marking control system, laser marking and the rotation shaft can work at the same time, and accordingly the problems that the rotation marking efficiency is low and the marking effect is not ideal are solved.
Owner:HANS LASER TECH IND GRP CO LTD

Three-dimensional laser marking method and device and storage medium

The invention relates to a three-dimensional laser marking method. The method comprises the steps that a three-dimensional rectangular coordinate system is established with the direction parallel to alaser beam as one coordinate axis, the focus depth of the laser beam is divided into N equal diversion points, and focus planes are established through the equal diversion points; a plurality of nodes are marked in each focus plane, and accordingly the focus planes are calibrated; the original coordinates of a marking point in the three-dimensional rectangular coordinate system are obtained, andthe node corresponding to the marking point serves as a correction node according to the marking point; according to the coordinates of the correction node, the coordinates of the marking point are corrected, and the corrected coordinates of the marking point are obtained; and the distance between the marking point and the laser emitting point is calculated according to the corrected coordinates of the marking point and the coordinates of the laser emitting point, and the distance is set as the actual focal length for marking. The invention further relates to a three-dimensional laser markingdevice and a storage medium. According to the three-dimensional laser marking method and device and the storage medium, a front focus galvanometer type marking system can obtain high machining precision, and the marking effect is good.
Owner:SHENZHEN TETELASER TECH CO LTD

Laser-marked glass-fiber reinforced polypropylene composite as well as preparation method and application thereof

The invention provides a laser-marked glass-fiber reinforced polypropylene composite which is characterized by comprising the following components in percentage by weight: 40%-80% of polypropylene resin, 5%-30% of elastomer, 5%-50% of glass fiber, 0.01%-10% of compatilizer, 0.01%-3% of black toner, and 0.01%-5% of other additives. A preparation method of the laser-marked glass-fiber reinforced polypropylene composite comprises the following steps: except the glass fiber, mixing the components except for the glass fiber in proportion and then feeding through a main feeding hole of a double-screw extruder or a reciprocating single-screw extruder; feeding the glass fiber through a side feeding hole of the extruder; fusing and mixing at the temperature of 200-280 DEG C for 0.1-5 minutes, wherein the length-diameter ratio of a screw is not less than 32-66; and then extruding and granulating, thus obtaining the laser-marked glass-fiber reinforced polypropylene composite. The laser-marked glass-fiber reinforced polypropylene composite has the advantages that the components can form light writings or patterns with strong contrast on a brunet polypropylene plastics surface, and marks do not disappear or fade after a long time under the action of mechanical, chemical or physical factors, thereby meeting the using requirements on plastic parts of industries of automobiles, household appliances, IT (information technology), office equipment, electric tools and the like; and the laser-marked glass-fiber reinforced polypropylene composite has the advantages of low cost, environmental conservation and strong environmental friendliness.
Owner:KINGFA SCI & TECH CO LTD +2

Fluorescence labeling method for cotton pollen tube microfilament framework

InactiveCN101477000AOmit the step of enzymatic digestionHigh fluorescence staining efficiencyPreparing sample for investigationZoologyRefrigerated temperature
The invention relates to a method for carrying out fluorescence labeling on an actin filament framework of a cotton pollen tube, which belongs to the technical field of biology. The method comprises: perforating a glass slide to make a chamber for cultivating the pollen tube; cultivating pollen in the chamber to bourgeon the pollen tube; fixing the cultivated pollen tube by 50mm Pipes buffer solution (pH6.9) containing 4 percent of paraformaldehyde for 1 to 2 h; washing the pollen tube, and putting the pollen tube into 0.1 to 0.2 percent glycerin (or 0.1 to 0.2 percent Triton X-100) for 3 to 4 h at a room temperature (or over night in a 4 DEG C refrigerator); washing the pollen tube, and staining the pollen tube through a phalloidine labeled by TRITC(rhodamine) with the concentration of 1 to 2.5 mu g / ml; and washing the pollen tube, and then observing the pollen tube under a confocal scanning microspope. The method has the advantages of simple operation, good labeling effect and important practical application value, and can play an important part in researching the framework of a cotton pollen tube cell and fertilization reproduction.
Owner:NANJING AGRICULTURAL UNIVERSITY

Image annotation method

The invention relates to an image annotation method comprising the following steps: 1, defining a target function of an image annotation model; 2, inputting an image into a CNN model so as to obtain original image characteristics; 3, weighting the original image characteristics; 4, inputting information into a LSTM model; 5, making reversed propagations for errors caused by prediction result. Theimage annotation method firstly uses a convolution nerve network to extract image bottom layer characteristics, then uses a focusing mechanism to extract image characteristics related to image annotation words in an image specific position area, inputting the information into the LSTM network model, forming corresponding prediction annotation words, and finally realizing image annotations; the image annotation method is excellent in annotation performance, high in annotation precision, thus well satisfying real application demands.
Owner:BEIJING INFORMATION SCI & TECH UNIV +1

Marking method, system and device of spliced wall display interface

The invention relates to a marking method, system and device of a spliced wall display interface. Moving trail coordinate data for marking the spliced wall display interface of a mouse is acquired, the moving trail coordinate data is converted into first trail coordinate data on a spliced wall display interface coordinate system according to the resolution ratio of the spliced wall display interface, then the first trail coordinate data is converted into second trail coordinate data on display interface coordinate systems of target display units on a spliced wall, and target pixel blocks of marked trails on the target display units are determined according to the second trail coordinate data and are marked. According to the scheme, a marked range can be the whole display interface of the spliced wall but is not limited to a window interface of a certain way of signal connected with the spliced wall, the marked application range of the spliced wall display interface is widened, and a marking effect is good.
Owner:GUANGDONG VTRON TECH CO LTD

Multifunctional marking machine

The invention discloses a multifunctional marking machine, and relates to the technical field of applying a laser to conduct pattern processing on fabrics. The multifunctional marking machine comprises a light path assembly, a lifting device, a working table, a preview device and a control system, wherein the light path assembly is arranged on the lifting device, workpieces to be processed are placed on the working table, the light path assembly is arranged above the working table, and the preview device can preview marking positions and patterns of the laser on the workpieces to be processed; the light path assembly comprises a laser generator arranged on a baseplate, a light path regulating and controlling device, a dynamic focusing device and a galvanometer device; the control system comprises a memory module and a control module, and the light path assembly, the lifting device and the preview device are in electrical connection with the control system. The laser engrave degree can be controlled dynamically, and the damage of fabric strength can be reduced to a minimum. A red light preview device is equipped to preview the marking positions and patterns, and the marking effect is improved. The degree of automation is high. The wide application is achieved, and marking is suitable for a variety of fabrics.
Owner:GBOS LASER INC

Neural network-based automatic image annotation method, system, device and medium

The invention relates to a neural network-based automatic image annotation method, a system, a device and a medium. The method comprises the steps of extracting image features of an experimental dataset by utilizing a pre-trained convolutional neural network model; according to the image features, calculating in a training set to obtain a neighborhood image set of the to-be-labeled image and a corresponding first label domain; constructing a label semantic association model between the first label domain and a second label domain corresponding to the training set, and performing calculation in the second label domain according to the label semantic association model to obtain a third label domain associated with each first label; calculating the similarity between the to-be-labeled imageand each neighborhood image, obtaining a first probability that each first label becomes a target label according to all the similarities, and obtaining a second probability that each third label becomes the target label according to all the first probabilities and the label semantic association model; and obtaining a target label according to all the similarities, all the first probabilities andall the second probabilities, and completing labeling according to the target label.
Owner:WUHAN INSTITUTE OF TECHNOLOGY +2

Gumnosperm pollen tube microfilament framework fluorescent marking method and its uses

InactiveCN1884486AOmit the step of enzymatic digestionAvoid damagePlant cellsCells boneFluorescence
The invention discloses a method for fluorescence labeling of gymnosperm pollen tube fibril bone and its application and is to provide a method for fluorescence labeling of gymnosperm pollen tube fibril bone and the application of this method in microscope inspection of gymnosperm pollen tube fibril bone. The said labelling method includes the following steps: 1) fixing the gymnosperm pollen tube with 40-60mM Pipes cushion solution containing 2-4 % cavaform; 2) washing the pollen tube; 3) placing the pollen tube in 0.2-0.3 % glycerin to infiltrate for 30-60min; 4) washing the pollen tube; 5) dyeing the pollen tube and avoiding the sun with 150-250nM phalloidine by fluorescence labeling for 0.5-1.5h and fluorescence labeling the fibril bone. The invention is of simple operation and is quick, has small damage to the cells, and has good labeling effect, which will play an important role in study gymnosperm pollen tube cell bone and have a high actual application value.
Owner:INST OF BOTANY CHINESE ACAD OF SCI

Fluorescence labeling method for free calcium ions of cotton pollen tube

The invention discloses a fluorescence labeling method for free calcium ions of a cotton pollen tube, and belongs to the technical field of biology. The fluorescence labeling method comprises the following steps: perforating glass slide to make a small chamber for culturing the pollen tube; culturing pollen in the small chamber, and sprouting the pollen tube; loading Fluo-3AM into the cultured pollen tube by a low temperature incubation method; and observing dynamic variation of Ca2+ in the pollen tube under a confocal laser scanning microscopy. The invention establishes a method for researching the concentration change of the Ca2+ in the cotton pollen tube by using the confocal laser microtechnique, has simple operation and good labeling effect, plays an important role in the growth of the cotton pollen tube and fertilization reproduction research, and has important practical application value.
Owner:NANJING AGRICULTURAL UNIVERSITY

Content markup method and system for digital work

The invention discloses a content markup method and system for a digital work. The content markup method and system for the digital work can enhance the ability of a work owner to mark up the content of the work based on inseparability of hybrid encoding of encrypted content and unencrypted content, and meanwhile the content distribution capacity and user-interface friendliness under an illegal authorization condition can be enhanced and improved. According to the technical scheme, a content processing terminal encrypts a specific digital work and performs hybrid encoding on the specific digital work and unencrypted digital content with markup information according to a certain rule, and then a composite digital work file in a specific format is generated. The composite digital work file can be used for being distributed to a user and being used through a client terminal, and the client terminal judges whether the user obtains legal use authorization of the specific digital work through a judging device or not. If the judgment result is positive, the specific encrypted digital work in the composite digital work file is decrypted and then the decrypted digital work is given to the user to be used; otherwise, the client terminal only sends the unencrypted digital content in the composite digital work file to the user to be used. The content markup method and system for the digital work are applied to distribution and operation fields of digital works.
Owner:上海精灵天下数字技术有限公司

Laser marking focus rapid positioning method and device

PendingCN108080794AExcellent laser marking effectImprove marking effectLaser beam welding apparatusOptoelectronicsLaser
A laser marking focus rapid positioning method comprises the steps that a distance measuring sensor is fixedly arranged on one side of a laser lens of a laser marking machine, and laser focusing is conducted on a to-be-marked object through the distance measuring sensor. The distance between the laser lens and a laser marking focus is determined directly through the distance measuring sensor, namely, the laser marking focus is determined, therefore, when the object to be subjected to laser marking is machined again, multiple times of marking experiments are not needed, and the optimal laser marking effect can be achieved only through the mode that the laser focus is moved to the surface of the object through the distance measuring sensor. The distance between the laser lens and the laser marking focus can be called, for short, as the laser marking focus distance. The direction of the distance measuring sensor is aligned with the center of a machining area, the laser lens focus is calibrated, and the numerical value of the distance measuring sensor is recorded.
Owner:Shandong Huaguang Optoelectronics Co. Ltd.

Mitochondrial fluorescence labeling method for angiosperm flower organs

InactiveCN103983496AEasy to markMark quick and fastPreparing sample for investigationFluorescence/phosphorescenceLaser scanningMitoTracker red CMXRos
The invention discloses a mitochondrial fluorescence labeling method for angiosperm flower organs. The method provided by the invention comprises the following steps: dyeing the treated angiosperm flower organs in a buffer solution containing Mitotracker Red CMXRos, thereby realizing fluorescence labeling of mitochondria of the angiosperm flower organs. An experiment proves that the mitochondrial fluorescence labeling method for angiosperm flower tepals has the advantages that a labeling effect is good, and uniform mitochondrion distribution around cells can be observed under a laser scanning confocal microscope; after being fixed by a fixing solution, a slide labeled by Mitotracker can be made into a permanent slide to be observed; mitochondria in the flower organs can be labeled simply, rapidly and quickly, a foundation is laid for distribution and displacement of the mitochondria of the flower organs, and the actual application value is high.
Owner:BEIJING FORESTRY UNIVERSITY

Cis-platinum complex and application thereof

The invention provides a cis-platinum complex, and also relates to a synthetic method of the cis-platinum complex and an application of the cis-platinum complex in preparing nucleotide and protein probes. The cis-platinum complex can be connected with fluorescein, and can also be firmly connected with DNA, RNA and proteins, so that the cis-platinum complex can be used as a connection main body of the biological probe and is suitable for marking and detecting the target DNA, RNA and protein; the cis-platinum complex is simple in marking operation procedures, and is not limited to the size of a marking molecule and not limited to a pH value of a marking mixed system; the cis-platinum complex can be widely applied to the subdivisions such as nucleotide, protein (antibody) and protein micro arrays, hybridization of comparative genome and the like; in addition, the marking efficiency is significantly improved, the marking time is shortened, the fluorescent intensity stability is improved, and the marking effect is excellent; and therefore, the application prospect in the fields such as preparation of nucleotide and protein probes, research and development of biological medicine, diagnosis of diseases and the like is good.
Owner:SHANGHAI TONGREN HOSPITAL

Special color master batch for black storage battery plastic slot and manufacturing method of special color master batch

The invention relates to a special color master batch for a black storage battery plastic slot and a manufacturing method of the special color master batch. The special color master batch is prepared from the following substances in parts by mass: 70-95 parts of carrier resin, 5-15 parts of a high-concentration black master batch, 0.5-2.5 parts of a dispersing agent, 0.5-2.5 parts of a compatilizer, 0.1-1 part of an antioxidant and 0.1-1 part of a laser marking agent, wherein the high-concentration black master batch contains the following components in parts by mass: 30-60 parts of carrier resin, 40-60 parts of carbon black, 0.8-2.8 parts of a dispersing agent, 0.8-2.8 parts of a compatilizer and 0.5-1 part of an antioxidant. The special color master batch is prepared by the steps of sampling, and carrying out secondary granulation. The special color master batch can be uniformly dispersed in a plastic matrix, so that the phenomenon of agglomeration of materials such as carbon black in the plastic matrix is avoided, and the injection-molded storage battery plastic slot can meet the requirement of high voltage breakdown resistance. Furthermore, by adding the laser marking agent into the color master batch, the marking effect of a storage battery plastic shell can be effectively improved.
Owner:FENGFAN
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