The invention discloses a ruthenium complex with aggregation state induced phosphorescence enhancement characteristics, its preparation method and application. The ruthenium complex has a structure shown in formula I: wherein, N^N bidentate ligand is the first ligand, X^X bidentate ligand is the second ligand, and the first ligand includes 2, 2-bipyridine or 1,10-phenanthroline, the second ligand includes 2,2-distyrylbenzene derivatives. The ruthenium complex can emit strong phosphorescence in an aggregated state, and when used to prepare electroluminescent devices, it is not necessary to dope it as a guest into the host material, thereby simplifying the device preparation process and reducing device manufacturing costs. The electroluminescence efficiency of the device is improved, which is beneficial to industrialization. At the same time, the method for preparing the ruthenium complex provided by the invention is simple, the conditions are easy to control, and it is convenient for large-scale implementation.