The invention provides a fixing device for assisting butt joint of PCBs. The fixing device comprises a first transverse frame, a first vertical frame, a second transverse frame and a second vertical frame which are sequentially connected in a head-tail mode to form a sealed frame and are mutually detachable. One side, facing the inner part of the sealed frame, of the first transverse frame is provided with a first fixing part for fixing a PCB; one side, facing the inner part of the sealed frame, of the second transverse frame is provided with a second fixing part for fixing the other PCB; one side, facing the inner part of the sealed frame, of the first vertical frame is provided with at least one first sliding groove for supporting side edges of the PCBs; and one side, facing the inner part of the sealed frame, of the second vertical frame is provided with second sliding grooves with the same number as the first sliding grooves and positions of the first sliding grooves and the second sliding grooves are mutual corresponding. The frames in head-tail connection are adopted for fixing the PCBs, the size of the frame is controlled to enable two or more PCBs to be in accurate butt joint, each spare part is fixed by adopting a bolt, the device is simple to assemble, the structure is firm, and the application range is wide.